Spleen cells from mice immunised with group-A type-M15 streptococci and boosted with purified IgG Fc-receptor (FcR) from this type were fused with Sp 2/0 mouse myeloma cells. The resulting hybridomas were screened by ELISA for antibody production. Two IgM-secreting cell lines were selected. The monoclonal antibodies and ascites fluids inhibited the binding of 125I-labelled human IgG and IgG Fc-fragments to group-A type-M15 streptococci. The monoclonal antibodies also displaced purified FcR towards the anode in electrophoresis. They opsonised group-A type M-15 streptococci for phagocytosis by human granulocytes in the presence of fresh human serum. It was concluded that FcR is important for group-A streptococcal virulence.
BurovaL. A.1980; Changes in virulence, M protein and IgG Fc-receptor activity in a type 12 group A streptococcal strain during mouse-passages. Acta Pathologica Microhiologica Scanilinavica Section B 88:199–205
BurovaL. A.1982; Streptococcal IgG Fc-receptor as a virulence factor. In
HolmS. E.,
ChristensenP.Basic concepts of streptococci and streptococcal diseases Reedbooks Ltd, Fox Lane North; Chertsey, Surrey. England: pp 205–206
Christensen SvenssonM. L.1979; Demonstration of the non-identity between the Fc receptor for human IgG from group A streptococci type 15 and M protein peptidoglycan and the group specific carbohydrate. Ada Pathologica Mtcrobiologica Scandinavica Section C 87:257–261
Christensen HolmS. E.1976; Purification of immunoglobulin G Fc-reactive factor from Streptococcus azgazardah
. Acta Pathologica Microhiologicu Scandinavica Section C 84:196–202
Christensen JohanssonB. G.,
KronvallG.1976; Interaction of streptococci with the Fc-fragment of IgG. Acta Pathologica Microhiologicu Scandinavica Section C 84:73–76
ChristensenP.,
OxeliusV. A.1974; Quantitation of the uptake of human IgG by some streptococci groups, A. B. C and G. Acta Pathologica Microbiologica Scandinavica Section B 82:475–483
FischerG. W.,
LowellG. H.,
CrumrineM. H.,
BassJ. W.1978; Demonstration of opsonic activity and in vivo protection against group B streptococci type III by Streptococcus pneumoniae type 14 anti-sera. Journal of Experimental Medicine 148:776–786
Galfre HoweS. C.,
MilsteinC.,
ButcherG. W.,
HowardJ. C.1977; Antibodies to major histocompatibility antigens produced by hybrid cellmis. Nature 266:550–552
GrubbA.,
ChristensenP.,
SchalénC.
1982; Isolation and some properties of an IgG Fc-binding protein from group A streptococci type 15. International Archives of Allergy and Applied Immunology 67:369–376
LancefieldR. C.1954; Cellular constituents of group A streptococci concerned in antigenecity and virulence. In
McCartyM.
(ed) Streptococcal infections Columbia University Press; New York: pp 3–18
LebrunL.,
PillotJ.,
Grangeot-KerosL.1982; Significance of anti-IgG antibodies obtained by immunization of rabbits with some streptococcal strains. Annales d’Immunologie (Institut Pasteur) 133C:45–56
LöfbergH.,
GrubbA.1979; Quantitation of γ-trace in human biological fluids: indication for production in the central nervous system. Scandinavian Journal of Clinical Laboratory Investigation 39:619–626
McConaheyP. J.,
DixonF. J.1966; A method of trace iodination of proteins for immunologic studies. International Archives of Allergy and Applied Immunology 29:185–189
MyhreE. B.,
KronvallG.1977; Heterogeneity of nonimmune immunoglobulin in Fc reactivity among gram-positive cocci: description of three major types of receptors for human immunoglobulin G. Infection and Immunity 17:475–482
ReisK. J.,
AyoubE. M.,
BoyleMD. P.1984a; Streptococcal Fc receptors. I. Isolation and partial characterization of the receptor from a group C streptococcus. II. Comparison of the reactivity of a receptor from a group C streptococcus with staphylococcal protein A. Journal of Immunology 132:3091–3097
ReisK. J.,
BoyleM. D. P.,
AyoubE. M.1984b; Identification of distinct Fc-receptor molecules on streptococci and staphylococci. Journal of Clinical and Laboratory Immunology 13:75–80
ReisK. J.,
YarnallM.,
AyoubE. M.,
BoyleMD. P.1984c; Effect of mouse passage on Fc receptor expression by group A streptococci. Scandinavian Journal of Immunology 20:433–439
SchalénC.,
ChristensenP.,
GrubbA.,
SamuelssonG.,
SvenssonM. L.1980; Demonstration of separate receptors for human IgA and IgG in group A streptococci type 4. Separation of the solubilized receptors from group- and type-specific antigens, lipoteichoic acid and peptidoglycan. Acta Pathologica Microbiologica Scandinavica Section C 88:77–82
SchalénC.,
BurovaL. A.,
ChristensenP.,
GrubbR.,
SvenssonM. L.1981; IgG F(ab′)2 of rabbit anti-M sera but not the unfractionated sera are bactericidal for some group A streptococci with IgG Fc-receptor activity: opsonic effects ascribable to anti-IgG. Acta Pathologica Microbiologica Scandinavica Section C 89:247–252
SchalénC.,
ZätterströmU.,
SvenssonM. L.,
ChristensenP.1983; Binding of aggregated IgG Fab-fragments and light chains to some group, A. group C and group G streptococci. Acta Pathologica Microbiologica Scandinavica Section B 91:27–33