. The effect of on the opsonisation and phagocytosis of gram-positive and gram-negative bacteria by human polymorphonuclear leukocytes (PMNL) was investigated. Uptake of most isolates of staphylococci, streptococci and clostridia by PMNL after opsonisation with serum treated with was largely unimpaired. The same treatment of serum before opsonisation of isolates of and resulted in the uptake by PMNL varying with individual isolates; a large reduction occurred with some and none with others. Treatment of serum with B. lipopolysaccharide before opsonisation of produced a marked reduction in uptake, whereas treatment with capsular polysaccharide had little effect.


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