1887

Abstract

Summary

The effect of human serum on was studied with serumsensitive and serum-resistant strains. The bactericidal effect of human serum on serumsensitive strains of depended on the activation of the classical complement pathway. The role of activation of the alternative pathway was less important. After incubation in sub-bactericidal concentrations of serum these strains were also easily phagocytosed by polymorphonuclear leukocytes (PMNL). Strains of of certain O-types required not only an intact classical pathway but also the presence of specific antibodies for effective killing by serum and effective phagocytosis by PMNL, despite rapid activation of complement and rapid deposition of C3 on the bacterial surface in the absence of antibody. Capsulate strains O1K1 and O78K80 resisted the bactericidal effect of serum even in the presence of specific antibodies; phagocytosis by PMNL only occurred after opsonisation with specific antibodies.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-22-2-143
1986-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/22/2/medmicro-22-2-143.html?itemId=/content/journal/jmm/10.1099/00222615-22-2-143&mimeType=html&fmt=ahah

References

  1. Fine D. P. 1981; Complement and infectious diseases. CRC Press; Inc., Boca Raton, FL. pp 43–84
    [Google Scholar]
  2. Forsgren A., Mclean R. H., Michael A. F., Quie P. G. 1975; Studies of the alternative pathway in chelated serum. Journal of Laboratory and Clinical Medicine 85:904–912
    [Google Scholar]
  3. Fraker P. J., Speck J. C. 1978; Protein and cell membraneiodinations with a sparingly soluble chloroamide, 1, 3, 4, 6- tetrachloro-3a, 6a-diphenylglycoluril. Biochemical and Biophysical Research Communications 80:849–857
    [Google Scholar]
  4. Glynn A. A., Howard C. J. 1970; The sensitivity to complement of strains of Escherichia coli related to their K antigens. Immunology 18:331–346
    [Google Scholar]
  5. Goldman J. N., Ruddy S., Austen K. F., Feingold D. S. 1969; The serum bactericidal reaction. III. Antibody and complement requirements for killing a rough Escherichia coli . Journal of Immunology 102:1379–1387
    [Google Scholar]
  6. Howard C. J., Glynn A. A. 1971; The virulence for mice of strains of Escherichia coli related to the effects of K antigens on their resistance to phagocytosis and killing by complement. Immunology 20:767–777
    [Google Scholar]
  7. Inoue K., Tanigawa Y., Takubo M., Satani M., Amano T. 1959; Quantitative studies on immune bacteriolysis. II. The role of lysozyme in immune bacteriolysis. Biken Journal 2:1–20
    [Google Scholar]
  8. Inoue K., Yonemasu K., Takamizawa A., Amano T. 1968; Studies on the immune bacteriolysis. XIV. Requirement of all nine components of complement for immune bacteriolysis. Biken Journal 11:203–206
    [Google Scholar]
  9. Joiner K. A., Goldman R. C., Hammer C. H., Leive L., Frank M. M. 1983a; Studies of the mechanism of bacterial resistance to complement-mediated killing. V. IgG and F(ab′)2 mediate killing of E. coli O111B4 by the alternative complement pathway without increasing C5b-9 deposition. The Journal of Immunology 131:2563–2569
    [Google Scholar]
  10. Joiner K. A., Goldman R. C., Hammer C. H., Leive L., Frank M. M. 1983b; Studies on the mechanism of bacterial resistance to complement-mediated killing. VI. IgG increases the bactericidal efficiency of C5b-9 for E. coli O111B4 by acting at a step before C5 cleavage. The Journal of Immunology 131:2570–2575
    [Google Scholar]
  11. Joiner K. A., Hammer C. H., Brown E. J., Cole R. J., Frank M. M. 1982a; Studies on the mechanism of bacterial resistance to complement-mediated killing. I. Terminal complement components are deposited and released from Salmonella minnesota S218 without causing bacterial death. Journal of Experimental Medicine 155:797–808
    [Google Scholar]
  12. Joiner K. A., Hammer C. H., Brown E. J., Frank M. M. 1982b; Studies on the mechanism of bacterial resistance to complement- mediated killing. II. C8 and C9 release C5b67 from the surface of Salmonella minnesota S218 because the terminal complex does not insert into the bacterial outer membrane. Journal of Experimental Medicine 155:809–819
    [Google Scholar]
  13. Klebanoff S. J. 1975; Antimicrobial systems of the polymorphonuclear leukocyte. In Bellanti J. A., Dayton D. H. (eds) The phagocytic cell in host resistance Raven Press; New York: p 45–59
    [Google Scholar]
  14. Mayer M. M. 1961; Complement and complement fixation. In Kabat E. A., Mayer M. M. (eds) Experimental immunochemistry 2nd edn Charles C. Thomas; Springfield, IL: pp 133–240
    [Google Scholar]
  15. Robbins J. B., McCracken G. H., Gotschlich E. C., Orskov F., Orskov I., Hanson L. A. 1974; Escherichia coli K1 capsular polysaccharide associated with neonatal meningitis. New England Journal of Medicine 290:1216–1220
    [Google Scholar]
  16. Roberts A. P., Philips R. 1981; The effects of ethyleneglycoltetraacetic acid on bactericidal activity of human serum against Escherichia coli . Journal of Medical Microbiology 14:195–203
    [Google Scholar]
  17. Roberts A. P., Phillips R. 1983; The relative importance of the classical and alternative complement pathway in serum bactericidal activity against Escherichia coli . Journal of Medical Microbiology 16:69–74
    [Google Scholar]
  18. Rowley D. 1973; Antibacterial action of antibody and complememt. Journal of Infectious Diseases 128: Suppl S170–S175
    [Google Scholar]
  19. Rozenberg-Arska M., Salters E. C., van Strijp J. A., Hoekstra W. P. M., Verhoef J. 1984; Degradation of Escherichia coli chromosomal and plasmid DNA in serum. Journal of General Microbiology 130:217–222
    [Google Scholar]
  20. Schreiber R. D., Morrison D. C., Podack E. R., Eberhard H. J. 1979; Bactericidal activity of the alternative complement pathway generated from eleven isolated plasma proteins. Journal of Experimental Medicine 149:870–882
    [Google Scholar]
  21. Taylor P. W. 1983; Bactericidal and bacteriolytic activity of serum against gram-negative bacteria. Microbiological Reviews 47:46–83
    [Google Scholar]
  22. Van Dijk W. C., Verburgh H. A., van der Tol M. E., Peters R., Verhoef J. 1981; Escherichia coli antibodies in opsonisation and protection against infection. Journal of Medical Microbiology 14:381–389
    [Google Scholar]
  23. Verhoef J., Peterson P. K., Quie P. G. 1977; Kinetics of Staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using [3H]thymidine labeled bacteria. Journal of Immunological Methods 14:303–311
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-22-2-143
Loading
/content/journal/jmm/10.1099/00222615-22-2-143
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error