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1887

Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 22, Issue 2

Antibacterial Immunity to in Rats No Access

Abstract

Summary

Blind loops prepared in the small intestines of fasted, MgSO-treated rats were shown to provide a simple, consistent and inexpensive means of studying mucosal colonisation by serogroup O1. When . 2000 cfu were injected, the number of mucosa-associated in each loop increased by . 5-6 orders of magnitude in 10-14 h, without enterotoxin-induced fluid production. Scanning electronmicroscopy and culture suggested that most surface-associated organisms were present in the adherent surface mucus. strains varied in terms of surface-colonising capacity.

Immunisation with given intra-intestinally greatly reduced the rate of increase and final number of mucosa-associated vibrios within the 14-h period after challenge. The method could be used to compare the immunity induced by various immunising regimens. Immunity was sometimes accompanied by intestinal mucusborne antibody against lipopolysaccharide but was sometimes demonstrated in the absence of such antibody or of mucus-borne antibody to heat-sensitive surface protein.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1986
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1986-09-01
2025-01-22
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References

  1. Attridge S. R., Rowley D. 1983; The role of the flagellum in the adherence of Vibrio cholerae . Journal of Infectious Diseases 147:864–872
     [Google Scholar]
  2. Cooper G. N., Jackson G. D. F. 1981; Immune responses of rats to live Vibrio cholerae: antibodies in serum and intestinal secretions. Parasite Immunology 3:57–68
     [Google Scholar]
  3. Cooper G. N., McNab C. E., Jackson G. D. F., Walker P. 1983; Enzyme–linked immunoassays for antibodies against Vibrio cholerae . Australian Journal of Experimental Biology and Medical Science 61:713–725
     [Google Scholar]
  4. Cooper G. N., McNab C. E., Walker P. G., Jackson G. D. F. 1984; Intestinal antibodies in rats following exposure to live Vibrio cholerae . Australian Journal of Experimental Biology and Medical Science 62:465–477
     [Google Scholar]
  5. Cooper G. N., Turner K. 1967; Immunological responses in rats following antigenic stimulation of Peyer’s patches. 1. Characteristics of the primary response. Australian Journal of Experimental Biology and Medical Science 45:363–378
     [Google Scholar]
  6. Cray W. C., Tokunaga E., Pierce N. F. 1983; Successful colonization and immunization of adult rabbits by oral inoculation with Vibrio cholerae Ol. Infection and Immunity 41:735–741
     [Google Scholar]
  7. Curlin G. T., Craig J. P., Subong A., Carpenter C. C. J. 1970; Antitoxic immunity in experimental canine cholera. Journal of Infectious Diseases 121:463–470
     [Google Scholar]
  8. Freter R. 1964; Comparison of immune mechanisms in various experimental models of cholera. Bulletin of the World Health Organization 31:825–834
     [Google Scholar]
  9. Freter R. 1969; Studies of the mechanism of action of intestinal antibody in experimental cholera. Texas Reports of Biology and Medicine 27: Suppl 1299–316
     [Google Scholar]
  10. Holmgren J., Svennerholm A. M. 1983; Cholera and the immune response. Progress in Allergy 33:106–119
     [Google Scholar]
  11. Jackson G. D. F., Cooper G. N. 1981; Immune responses of rats to live Vibrio cholerae: secretion of antibodies in bile. Parasite Immunology 3:127–135
     [Google Scholar]
  12. Kerss S., Allen A., Garner A. 1982; A simple method for measuring thickness of the mucus gel layer adherent to rat, frog and human gastric mucosa; influence of feeding, prostaglandin, N-acetylcysteine and other agents. Clinical Science (London) 63:187–195
     [Google Scholar]
  13. Lange S. 1982; A rat model for an in vivo assay of enterotoxic diarrhea. FEMS Microbiology Letters 15:239–242
     [Google Scholar]
  14. Lange S., Lonnroth I., Nygren H. 1984; Protection against experimental cholera in the rat: A study on the formation of antibodies against cholera toxin and desensitization of adenylate cyclase after immunization with cholera toxin. International Archives of Allergy and Applied Immunology 75:143–149
     [Google Scholar]
  15. Levine M. M., Kaper J. B., Black R. E., Clements M. L. 1983; New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiological Reviews 47:510–550
     [Google Scholar]
  16. Levine M. M., Nalin D. R., Craig J. P., Hoover D., Bergquist E. J., Waterman D., Holley H. P., Homick R. B., Pierce N. F., Libonati J. P. 1979; Immunity of cholera in man: relative role of antibacterial versus antitoxic immunity. Transactions of the Society of Tropical Medicine and Hygiene 73:3–9
     [Google Scholar]
  17. Mosley W. H., Benenson A. S., Barui R. 1968; A serological survey for cholera antibodies in rural East Pakistan. I. The distribution of antibody in the control population of a cholera–vaccine field-trial area and the relation of antibody titre to the pattern of endemic cholera. Bulletin of the World Health Organization 38:327–334
     [Google Scholar]
  18. Robertson P. W., Cooper G. N. 1972; Immune responses in intestinal tissues to particulate antigens. Plaque-forming and rosette-forming cell responses in rats. Australian Journal Experimental Biology and Medical Science 50:703–714
     [Google Scholar]
  19. Schaedler R. W., Dubos R., Costello R. 1965; The development of the bacterial flora in the gastrointestinal tract of mice. Journal of Experimental Medicine 122:59–66
     [Google Scholar]
  20. Spira W. M., Sack R. B., Froehlich J. L. 1981; Simple adult rabbit model for Vibrio cholerae and enterotoxigenic Escherichia coli diarrhea. Infection and Immunity 32:739–747
     [Google Scholar]
  21. Tokunaga E., Cray W. C., Pierce N. F. 1984; Compared colonizing and immunizing efficiency of toxinogenic (A+.B+.) Vibrio cholerae and an A−B+. mutant (Texas Star—SR) studied in adult rabbits. Infection and Immunity 44:364–369
     [Google Scholar]
  22. Ujiiye A., Kobari K. 1970; Protective effect on infections with Vibrio cholerae in suckling mice caused by the passive immunization with milk of immune mothers. Journal of Infectious Diseases 121: (Suppl.) S50–S55
     [Google Scholar]
  23. Yancey R. J., Willis D. L., Berry L. J. 1978; Role of motility in experimental cholera in adult rabbits. Infection and Immunity 22:387–392
     [Google Scholar]
/content/journal/jmm/10.1099/00222615-22-2-133
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Antibacterial Immunity to Vibrio Cholerae in Rats
J. Med. Microbiol. 22, 133 (1986); https://doi.org/10.1099/00222615-22-2-133
/content/journal/jmm/10.1099/00222615-22-2-133
/content/journal/jmm/10.1099/00222615-22-2-133
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