1887

Abstract

Summary

A series of 178 strains of , highly resistant to trimethoprim, isolated from hospital patients and patients in the community between 1979 and 1983, was examined for the presence of Tn7 on a plasmid or on the chromosome only, by transposition to RP4 and restriction endonuclease digestion with d III. Of the isolates, 57% carried Tn7. Comparison of hospital isolates from 1979 to 1980 and 1982 showed that although the proportions that carried Tn7 were similar (63% and 57%) there had been a significant change in the genetic location of the transposon. The proportion of plasmid-mediated Tn7 had fallen from 62% to 30% with a corresponding rise in Tn7 located exclusively on the chromosome from 38% to 70%. This change may be the result of continuing transposition of Tn7 from plasmids to the bacterial chromosome followed by plasmid loss. The consequent reduction in the mobility of trimethoprim-resistance genes may in turn lead to changes in the incidence of resistance.

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1986-09-01
2024-04-23
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References

  1. Bachmann B. J. 1972; Pedigrees of some mutant strains of Escherichia coli K12. Bacteriological Reviews 36:525–557
    [Google Scholar]
  2. Barth P. T., Datta N., Hedges R. W., Grinter N. J. 1976; Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons. Journal of Bacteriology 125:800–810
    [Google Scholar]
  3. Barth P. T., Datta N. 1977; Two naturally occurring transposons indistinguishable from Tn7. Journal of General Microbiology 102:129–134
    [Google Scholar]
  4. Barth P. T., Richards H., Datta N. 1978; Copy numbers of coexisting plasmids in Escherichia coli K12. Journal of Bacteriology 135:760–765
    [Google Scholar]
  5. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  6. Bradley D. E., Taylor D. E., Cohen D. R. 1980; Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K12. Journal of Bacteriology 143:1466–1470
    [Google Scholar]
  7. Datta N., Hughes V. M., Nugent M. E., Richards H. 1979; Plasmids and transposons and their stability and mutability in bacteria isolated during an outbreak of hospital infection. Plasmid 2:182–196
    [Google Scholar]
  8. Datta N., Nugent M., Richards H. 1981; Transposons encodintrimethoprim or gentamicin resistance in medically important bacteria. Cold Spring Harbor Symposia on Quantitative Biology XLV:45–51
    [Google Scholar]
  9. Datta N., Richards H. 1981; Trimethoprim resistant bacteria in hospital and in the community: spread of plasmids and transposons. In Levy S. B. (eds) Molecular biology, pathogenicity and ecology of bacterial plasmids Plenum Press; New York: pp 21–30
    [Google Scholar]
  10. Dornbusch K., Hagelberg A. 1983; Transferable trimethoprim resistance in urinary tract pathogens isolated in Finland and Sweden. Scandinavian Journal of Infectious Diseases 15:285–291
    [Google Scholar]
  11. Fling M. E., Walton L., Elwell L. P. 1982; Monitoring of plasmid-encoded, trimethoprim-resistant dihydrofolate reductase genes: detection of a new resistant enzyme. Antimicrobial Agents and Chemotherapy 22:882–888
    [Google Scholar]
  12. Ish-Horowicz D., Burke J. F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989–2998
    [Google Scholar]
  13. Kraft C. A., Platt D. J., Timbury M. C. 1983; Distribution and transferability of plasmids in trimethoprim resistant urinary Escherichia coli . Journal of Medical Microbiology 16:433–441
    [Google Scholar]
  14. Kraft C. A., Platt D. J., Timbury M. C. 1984; Distribution and transferability of plasmids in trimethoprim-resistant urinary strains of Escherichia coli: a comparative study of hospital isolates. Journal of Medical Microbiology 18:95–105
    [Google Scholar]
  15. Kraft C. A., Platt D. J., Timbury M. C. 1985; Trimethoprim resistance in urinary coliforms from patients in the community: plasmids and R-transfer. Journal of Antimicrobial Chemotherapy 15:311–317
    [Google Scholar]
  16. Platt D. J., Sommerville J. S. 1981; A simple method for the detection of resistance plasmids in Serratia species by transfer to members of the genus Enterobacter . Journal of Antimicrobial Chemotherapy 8:145–152
    [Google Scholar]
  17. Richards H., Nugent M. 1979; The incidence and spread of transposon 7. In Timms K. W., Puhler A. (eds) Plasmids of medical, environmental and commercial importance Elsevier/North Holland Biomedical Press; pp 195–198
    [Google Scholar]
  18. Shapiro J. A., Sporn P. 1977; Tn402: a new transposable element determining trimethoprim resistance that inserts in bacteriophage lambda. Journal of Bacteriology 129:1632–1635
    [Google Scholar]
  19. Sherratt D. J. 1982; The maintenance and propagation of plasmid genes in bacterial populations. Journal of General Microbiology 128:655–661
    [Google Scholar]
  20. Siegel S. 1956; Non parametric statistics for the behavioural sciences. McGraw Hill Book Company Inc; New York:
    [Google Scholar]
  21. Towner K. J., Pearson N. J., Pinn P. A., O’Grady F. 1980; Increasing importance of plasmid-mediated trimethoprim resistance in enterobacteria: two six month clinical surveys. British Medical Journal 280:517–519
    [Google Scholar]
  22. Towner K. J. 1982; Resistance to trimethoprim among urinary tract isolates in the United Kingdom. Reviews of Infectious Diseases 4:456–460
    [Google Scholar]
  23. Towner K. J., Venning B. M., Pinn P. A. 1982; Occurrence of transposable trimethoprim resistance in clinical isolates of Escherichia coli devoid of self-transmissible resistance plasmids. Antimicrobial Agents and Chemotherapy 21:336–338
    [Google Scholar]
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