Skip to content
1887

Abstract

Summary

Chloramphenicol resistance (Cm) plasmids pSK2 and pSK5 from and pSK 102 and pSK 103 from have been characterised and detailed restriction endonuclease cleavage maps constructed. I digestion profiles illustrated the identity of pSK 5 and pSK 102 and also revealed a high degree of similarity between these four Cm plasmids from Australian staphylococci and three Cm plasmids from strains of geographically unrelated origin. DNA-DNA hybridisation indicated that the chloramphenicol acetyltransferase determinant carried by pSK 5/pSK 102 could be found on other structurally-distinct Cm plasmids. The role of as a reservoir for Cm plasmids found in is discussed.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-22-1-79
1986-08-01
2025-01-25
Loading full text...

Full text loading...

References

  1. Archer G. L., Johnston J. L. 1983; Self-transmissible plasmids in staphylococci that encode resistance to aminoglycosides. Antimicrobial Agents and Chemotherapy 24:70–77
    [Google Scholar]
  2. Brenner D. G., Shaw W. V. 1985; The use of synthetic oligonucleotides with universal templates for rapid DNA sequencing: results with staphylococcal replicon pC221. EM BO Journal 4:561–568
    [Google Scholar]
  3. Brückner R., Zyprian E., Matzura H. 1984; Expression of a chloramphenicol-resistance determinant carried on hybrid plasmids in Gram-positive and Gram-negative bacteria. Gene 32:151–160
    [Google Scholar]
  4. Cohen M. L., Wong E. S., Falkow S. 1982; Common R-plasmids in Staphylococcus aureus and Staphylococcus epidermidis during a nosocomial Staphylococcus aureus outbreak. Anti-microbial Agents and Chemotherapy 21:210–215
    [Google Scholar]
  5. Cooksey R. C., Baldwin J. N. 1985; Relatedness of tetracycline resistance plasmids among species of coagulase-negative staphylococci. Antimicrobial Agents and Chemotherapy 27:234–238
    [Google Scholar]
  6. Daniels D. L., Schroeder J. L., Szybalski W., Sanger F., Blattner F. R. 1983; A molecular map of coliphage lambda. In Hendrix R. W., Roberts J. W., Stahl F. W., Weisberg R. A. (eds) Lambda II Cold Spring Harbor Laboratory, Cold Spring Harbor; New York: p 469–517
    [Google Scholar]
  7. Gillespie M. T., May J. W., Skurray R. A. 1984; Antibiotic susceptibilities and plasmid profiles of nosocomial methicillin-resistant Staphylococcus aureus: a retrospective study. Journal of Medical Microbiology 17:295–310
    [Google Scholar]
  8. Groves D. J. 1979; Interspecific relationships of antibiotic resistance in Staphylococcus sp.: isolation and comparison of plasmids determining tetracycline resistance in 5. aureus and S. epidermidis. Canadian Journal of Microbiology 25:1468–1475
    [Google Scholar]
  9. Iordănescu S., Surdeanu M., Della Latta P., Novick R. 1978; Incompatability and molecular relationships between small staphylococcal plasmids carrying the same resistance marker. Plasmid 1:468–479
    [Google Scholar]
  10. Jaffe H. W., Sweeney H. M., Weinstein R. A., Rabins S. A., Nathan C., Cohen S. 1982; Structural and phenotypic varieties of gentamicin resistance plasmids in hospital strains of Staphylococcus aureus and coagulase-negative staphylococci. Anti-microbial Agents and Chemotherapy 21:773–779
    [Google Scholar]
  11. Lyon B. R., Luorio J. L., May J. W., Skurray R. A. 1984a; Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals. Journal of Medical Microbiology 17:79–89
    [Google Scholar]
  12. Lyon B. R., May J. W., Skurray R. A. 1983; Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 23:817–826
    [Google Scholar]
  13. Lyon B. R., May J. W., Skurray R. A. 1984b; Tn4001, a gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Molecular and General Genetics 193:554–556
    [Google Scholar]
  14. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor; New York:
    [Google Scholar]
  15. McDonnell R. W., Sweeney H. M., Cohen S. 1983; Conjugational transfer of gentamicin resistance plasmids intra-and inter-specifically in Staphylococcus aureus and Staphylococcus epidermidis. Antimicrobial Agents and Chemotherapy 23:151–160
    [Google Scholar]
  16. Naidoo J. 1984; Interspecific co-transfer of antibiotic resistance plasmids in staphylococci in vivo. Journal of Hygiene Cambridge: 93:59–66
    [Google Scholar]
  17. Novick R. P. 1976; Plasmid-protein relaxation complexes in Staphylococcus aureus. Journal of Bacteriology 127:1177–1187
    [Google Scholar]
  18. Projan S. J., Kornblum J., Moghazeh S. L., Edelman I., Gennaro M. L., Novick R. P. 1985; Comparative sequence and functional analysis of pT181 and pC221, cognate plasmid replicons from Staphylococcus aureus. Molecular and General Genetics 199:452–464
    [Google Scholar]
  19. Shaw W. V. 1984; Bacterial resistance to chloramphenicol. British Medical Bulletin 40:36–41
    [Google Scholar]
  20. Smith H. O. 1980; Recovery of DNA from gels. Methods in Enzymology 65:371–380
    [Google Scholar]
  21. Smith G. E., Summers M. D. 1980; The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Analytical Biochemistry 109:123–129
    [Google Scholar]
  22. Sutcliffe J. G. 1978; pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361. nucleotide pairs long. Nucleic Acids Research 5:2721–2728
    [Google Scholar]
  23. Tennent J. M., May J. W., Skurray R. A. 1984; Multiple antibiotic resistance in Staphylococcus aureus and Staphylococcus epidermidis: plasmids in strains associated with nosocomial infection. Pathology 16:250–255
    [Google Scholar]
  24. Totten P. A., Vidal L., Baldwin J. N. 1981; Penicillin and tetracycline resistance plasmids in Staphylococcus epidermidis. Antimicrobial Agents and Chemotherapy 20:359–365
    [Google Scholar]
  25. Wilson C. R., Baldwin J. N. 1978; Characterization and construction of molecular cloning vehicles within Staphylococcus aureus. Journal of Bacteriology 136:402–413
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-22-1-79
Loading
/content/journal/jmm/10.1099/00222615-22-1-79
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error