1887

Abstract

Summary

A cluster of infections caused by gentamicin-resistant gram-negative bacilli in a District General Hospital was investigated. The plasmids coding for gentamicin resistance in the infecting organisms and in isolates obtained from the ward environment and from the faeces of patients and staff were characterised. Six plasmids encoding gentamicin resistance were recognised amongst the organisms causing infection. Two of the plasmids were found in different serotypes of the same species and one plasmid was found in different genera. Three of the plasmids present in the organisms causing the infections were also present in the inanimate environment or in the bowel flora of patients and these also were found in different serotypes of the same species and in different genera.

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1986-08-01
2024-02-28
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References

  1. Anderson J. D., Gillespie W. A., Richmond M. H. 1973; Chemotherapy and antibiotic-resistance transfer between enterobacteria in the human gastro-intestinal tract. Journal of Medical Microbiology 6:461–473
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Casewell M. W., Talsania H. G., Knight S. 1981; Gentamicin-resistant Klebsiella aerogenes as a clinically significant source of transferable antibiotic resistance. Journal of Antimicrobial Chemotherapy 8:153–160
    [Google Scholar]
  4. Datta N., Cacey S., Hughes V., Knight S., Richards H., Williams G., Casewell M., Shannon K. P. 1980; Distribution of genes for trimethoprim and gentamicin resistance in bacteria and their plasmids in a general hospital. Journal of General Microbiology 118:495–508
    [Google Scholar]
  5. Frost J. A., Rowe B., Ward L. R., Threlfall E J. 1982; Characterization of resistance plasmids and carried phages in an epidemic clone of multi-resistant Salmonella typhimurium in India. Journal of Hygiene 88:193–204
    [Google Scholar]
  6. Maki D. G., Alvardo C. J., Hassemer C. A., Zilz M. A. 1982; Relation of the inanimate hospital environment to endemic nosocomial infection. New England Journal of Medicine 307:1562–1566
    [Google Scholar]
  7. Milch H., Ke N. T. 1981; Klebsiella and Enterobacter strains derived from hospital infections. 2. Occurrence and characterization of R-plasmids, LAC plasmids and COL plasmids and their clinical epidemiological significance. Acta Micro-biologica Academiae Scientiarum Hungaricae 28:171–196
    [Google Scholar]
  8. O’Brien T. F., Ross D. G., Guzman M. A., Medeiros A. A., Hedges R. W., Botstein D. 1980; Dissemination of an antibiotic resistance plasmid in hospital patient flora. Antimicrobial Agents and Chemotherapy 17:537–543
    [Google Scholar]
  9. Petrocheilou V., Grinsted J., Richmond M. H. 1976; R-plasmid transfer in vivo in the absence of antibiotic selection pressure. Antimicrobial Agents and Chemotherapy 10:753–761
    [Google Scholar]
  10. Rennie R. P., Duncan I. B. R. 1977; Emergence of gentamicin-resistant Klebsiella in a general hospital. Antimicrobial Agents and Chemotherapy 11:179–184
    [Google Scholar]
  11. Rubens C. E., Farrar W. E., McGee Z. A., Schaffner W. 1981; Evolution of a plasmid mediating resistance to multiple antimicrobial agents during a prolonged epidemic of nosocomial infections. Journal of Infectious Diseases 143:170–181
    [Google Scholar]
  12. Tompkins L. S., Plorde J. J., Falkow S. 1980; Molecular analysis of R-factors from multiresistant nosocomial isolates. Journal of Infectious Diseases 141:625–636
    [Google Scholar]
  13. Wenzel R. P., Veazey J. M., Townsend T. R. 1977; Role of the inanimate environment in hospital-acquired infections. Cundy K. R., Ball W. (eds) In: Infection control in health care facilities: microbiological surveillance University Park Press; Baltimore, MD: pp 71–143
    [Google Scholar]
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