Opsonic Requirements of Free

Abstract

Summary

The opsonic requirements of 18 strains of were compared in pooled normal human serum and in peritoneal dialysate from patients undergoing continuous ambulatory peritoneal dialysis (CAPD). A serum concentration of 2.5% gave optimal opsonisation. The opsonisation of all strains was antibody-and complement-dependent, and there were no significant differences in the pattern of their opsonic requirements. Peritoneal-dialysis effluent from uninfected patients was a poor source of opsonins because of the low levels of immunoglobulin G and of the C3 component of complement it contained. Growth of in peritoneal-dialysis effluent rather than in broth did not alter its opsonic requirements. Strains from patients undergoing CAPD and suffering from peritonitis were not more resistant to opsonisation and phagocytic killing than those from other sources.

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1986-08-01
2024-03-29
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References

  1. Baird-Parker A. C. 1963; A classification of micrococci and staphylococci based on physiological and biochemical tests. Journal of General Microbiology 30:409–427
    [Google Scholar]
  2. Bayston R., Penny S. R. 1972; Excessive production of mucoid substance in staphylococcus SIIA: a possible factor in colonisation of Holter shunts. Developmental Medicine and Child Neurology 14: (Suppl 27) 25–28
    [Google Scholar]
  3. Duwe A. K., Vas S. I., Weatherhead J. W. 1981; Effects of the composition of peritoneal dialysis fluid on chemiluminescence, phagocytosis and bactericidal activity in vitro. Infection and Immunity 33:130–135
    [Google Scholar]
  4. Easmon C. S. F., Glynn A. A. 1976; Comparison of subcutaneous and intraperitoneal staphylococcal infections in normal and complement-deficient mice. Infection and Immunity 13:399–406
    [Google Scholar]
  5. Hastings M. J. G., Easmon C. S. F. 1981; Variations in the opsonic requirements of group B streptococcus type III. British Journal of Experimental Pathology 62:519–525
    [Google Scholar]
  6. Keane W. F., Comty C. M., Verbrugh H. A., Peterson P. K. 1984; Opsonic deficiency of peritoneal dialysis effluent in continuous ambulatory peritoneal dialysis. Kidney International 25:539–543
    [Google Scholar]
  7. Keane W. F., Peterson P. K. 1984; Host defence mechanisms of the peritoneal cavity and continuous ambulatory peritoneal dialysis. Peritoneal Dialysis Bulletin 4:122–127
    [Google Scholar]
  8. Marples R. R., Hone R., Notley C. M., Richardson J. F., Crees-Morris J. A. 1978; Investigation of coagulase-negative staphylococci from infections in surgical patients. Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene I. Abt. Originale A 241:140–156
    [Google Scholar]
  9. Peters G., Locci R., Pulverer G. 1982; Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters. Journal of Infectious Diseases 146:479–482
    [Google Scholar]
  10. Peterson P. K., Wilkinson B. J., Kim Y., Schmeling D., Douglas S. D., Quie P. G. 1978; The key role of peptidoglycan in the opsonization of Staphylococcus aureus. Journal of Clinical Investigation 61:597–609
    [Google Scholar]
  11. Schliefer K. H. 1983; The cell envelope. In Easmon C. S. F., Adlam C. (eds) Staphylococci and staphylococcal infections vol 2: Academic Press Inc Ltd; London: pp 385–428
    [Google Scholar]
  12. Verbrugh H. A., Keane W. F., Hoidal J. R., Freiberg M. R., Elliott G. R., Peterson P. K. 1983; Peritoneal macrophages and opsonins: antibacterial defense in patients undergoing chronic peritoneal dialysis. Journal of Infectious Diseases 147:1018–1029
    [Google Scholar]
  13. Verbrugh H. A., Keane W. F., Conroy W. E., Peterson P. K. 1984; Bacterial growth and killing in chronic ambulatory peritoneal dialysis fluids. Journal of Clinical Microbiology 20:199–203
    [Google Scholar]
  14. Verhoef J., Peterson P. K., Kim Y., Sabath L. D., Quie P. G. 1977; Opsonic requirements for staphylococcal phagocytosis. Immunology 33:191–197
    [Google Scholar]
  15. Wheat L. J., Humphreys D. W., White A. 1974; Opsonization of staphylococci by normal human sera: the role of antibody and heat-labile factors. Journal of Laboratory and Clinical Medicine 83:73–78
    [Google Scholar]
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