1887

Abstract

Summary

Two hundred and eighty-seven coliform bacteria were isolated from 116 rectal swabs or faecal specimens obtained from 113 patients. By means of plasmid analysis and resistance transfer (R-transfer) was found to differ from other enteric genera in plasmid distribution. Criteria were proposed that enabled in-vivo "R-transfer potential" and in-vivo "R-transfer rate" to be calculated. From each of 22 of the 113 patients numerous coliforms were isolated, of which at least one per patient contained one or more self-transmissible R-plasmids potentially transferable to 43 other coliforms. Evidence indicated that R-plasmid transfer had occurred on two of the 43 potential occasions. These results are discussed in the context of plasmid ecology in the human host.

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1986-06-01
2022-05-29
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References

  1. Anderson J D. 1975; Factors that may prevent transfer of antibiotic resistance between Gram-negative bacteria in the gut. Journal of Medical Microbiology 8:83–88
    [Google Scholar]
  2. Bachmann B J. 1972; Pedigrees of some mutant strains ofEscherichia coli K-12. Bacteriological Reviews 36:525–557
    [Google Scholar]
  3. Birnboim H C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  4. Casewell M. 1982; The role of multiply resistant coliforms in hospital-acquired infection. In Reeves D S., Geddes A M. eds Recent advances in infection 2. Churchill Livingstone; Edinburgh: pp 31–50
    [Google Scholar]
  5. Datta N., Hughes V M. 1983; Plasmids of the same Inc groups in Enterobacteria before and after the medical use of antibiotics. Nature 306:616–617
    [Google Scholar]
  6. Datta N., Richards H., Datta C. 1981; Salmonella typhi in vivo acquires resistance to both chloramphenicol and co-trimox- azole. Lancet 1:1181–1183
    [Google Scholar]
  7. Hartley C L., Richmond M H. 1975; Antibiotic resistance and the survival of E. coli in the alimentary tract. British Medical Journal 4:71–74
    [Google Scholar]
  8. Hughes V M., Datta N. 1983; Conjugative plasmids in bacteria of the ‘pre-antibiotic’ era. Nature 302:725–726
    [Google Scholar]
  9. Ish-Horowitz D., Burke J F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989–2998
    [Google Scholar]
  10. Jarolmen H., Kemp G. 1969; R-factor transmission in vivo. Journal of Bacteriology 99:487–490
    [Google Scholar]
  11. Knight S., Casewell M. 1981; Dissemination of resistance plasmids among gentamicin-resistant enterobacteria from hospital patients. British Medical Journal 283:755–756
    [Google Scholar]
  12. Kraft C A., Platt D J., Timbury M C. 1983; Distribution and transferability of plasmids in trimethoprim resistant urinary Escherichia coli. Journal of Medical Microbiology 16:433–441
    [Google Scholar]
  13. Kraft C A., Platt D J., Timbury M C. 1984; Distribution and transferability of plasmids in trimethoprim-resistant urinary strains of Escherichia coli: a comparative study of hospital isolates. Journal of Medical Microbiology 18:95–105
    [Google Scholar]
  14. Petrocheilou V., Grinsted J., Richmond M H. 1976; R-plasmid transfer in vivo in the absence of antibiotic selection pressure. Antimicrobial Agents and Chemotherapy 10:753–761
    [Google Scholar]
  15. Platt D J. 1983; Bacterial plasmids and their fingerprints. Disease Markers 1:107–115
    [Google Scholar]
  16. Platt D J., Sommerville J S. 1981a; Serratia species isolated from patients in a general hospital. Journal of Hospital Infection 2:341–348
    [Google Scholar]
  17. Platt D J., Sommerville J S. 1981b; A simple method for the detection of resistance plasmids in Serratia species by transfer to member of the genus Enterobacter. Journal of Antimicrobial Chemotherapy 8:145–152
    [Google Scholar]
  18. Platt D J., Sommerville J S., Gribben J. 1984a; Sequential acquisition of R-plasmids in vivo by Salmonella typhimurium. Journal of Antimicrobial Chemotherapy 13:65–69
    [Google Scholar]
  19. Platt D J., Sommerville J S., , Mc Groarty J A. 1983; Characterization of spontaneous resistant variants of Serratia marces- cens selected in the presence of carbenicillin. Journal of Antimicrobial Chemotherapy 12:329–335
    [Google Scholar]
  20. Platt D J., Sommerville J S., Kraft C A., Timbury M C. 1984b; Antimicrobial resistance and the ecology of Escherichia coli plasmids. Journal of Hygiene 93:181–188
    [Google Scholar]
  21. Richmond M H., Petrocheilou V. 1978; R-factor transfer in vivo in humans. In Schlessinger D. ed Microbiology. American Society of Microbiology; Washington DC: pp 273–276
    [Google Scholar]
  22. Sherratt D J. 1982; The maintenance and propagation of plasmid genes in bacterial populations. Journal of General Microbiology 128:655–661
    [Google Scholar]
  23. Siegel S. 1956; Non-parametric statistics for the behavioral sciences. McGraw Hill Book Company Inc; New York:
    [Google Scholar]
  24. Smith H W. 1971; Observations on the in vivo transfer of R factors. Annals of the New York Academy of Sciences 182:80–90
    [Google Scholar]
  25. Smith H W. 1976; Mutants of Klebsiella pneumoniae resistant to several antibiotics. Nature 259:307–308
    [Google Scholar]
  26. Stuart-Harris C H., Harris D M. 1982; The control of antibiotic- resistant bacteria. Academic Press; New York:
    [Google Scholar]
  27. Zünd P., Lebek G. 1980; Generation time-prolonging R-plasmids: correlation between increases in the generation time of Escherichia coli caused by R-plasmids and their molecular size. Plasmid 3:65–69
    [Google Scholar]
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