1887

Abstract

Summary

The importance of K antigen of as a virulence factor was studied in nine pairs of K and K strains, each pair isogenic apart from the presence of K antigen. Loss of K antigen by nine K strains resulted in the reduced virulence of their K variants in a mouse-skin model. This reduced virulence of K strains for mice may be explained in all strains by a higher degree of phagocytosis as measured by chemiluminescence response of human polymorphonuclear leukocytes (PMNL) and in most strains by enhanced killing by either human PMNL or human serum or both. Although the protective role of the K antigen in serum-induced killing and killing by PMNL was generally evident, our results also suggested that other virulence factors were sometimes involved.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-21-2-133
1986-03-01
2022-05-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/21/2/medmicro-21-2-133.html?itemId=/content/journal/jmm/10.1099/00222615-21-2-133&mimeType=html&fmt=ahah

References

  1. Baerthlein K. 1918; Ueber bakterielle Variabilitåt insbesondere sogenannte Bakterienmutationen. Zentralblatt fiir Bakteriologe I.Abt.Orig. 81:369–435
    [Google Scholar]
  2. Cryz S. J., Fürer E., Germanier R. 1984; Experimental Klebsiella pneumoniae burn wound sepsis: role of capsular polysaccharide. Infection and Immunity 43:440–441
    [Google Scholar]
  3. Domenico P., Johanson W. G., Straus D. C. 1982; Lobar pneumonia in rats produced by clinical isolates of Klebsiella pneumoniae. Infection and Immunity 37:327–335
    [Google Scholar]
  4. Edwards P. R. 1928; The relation of encapsulated bacilli found in metritis in mares to encapsulated bacilli from human sources. Journal of Bacteriology 15:245–266
    [Google Scholar]
  5. Ehrenworth K., Baer H. 1956; The pathogenicity of Klebsiella pneumoniae for mice: the relationship to the quantity and rate of production of type specific capsular polysaccharide. Journal of Bacteriology 72:713–717
    [Google Scholar]
  6. Glynn A. A., Brumfitt W., Howard C. J. 1971; K antigens of Escherichia coli and renal involvement in urinary-tract infections. Lancet 1:514–516
    [Google Scholar]
  7. Hall H. E., Humphries J. C. 1958; The relationship between insusceptibility to phagocytosis and virulence of certain Klebsiella pneumoniae strains. Journal of Infectious Diseases 103:157–162
    [Google Scholar]
  8. Howard C. J., Glynn A. A. 1971; The virulence for mice of strains of Escherichia coli related to the effects of K antigens on their resistance to phagocytosis and killing by complement. Immunology 20:767–777
    [Google Scholar]
  9. McCabe W. R., Carling P. C., Bruins S., Greely A. 1975; The relation of K antigen to virulence of Escherichia coli. Journal of Infectious Diseases 131:6–10
    [Google Scholar]
  10. MacLeod C. M., Krauss M. R. 1950; Relation of virulence of pneumococcal strains for mice to the quantity of capsular polysaccharide formed in vitro. Journal of Experimental Medicine 92:1–9
    [Google Scholar]
  11. Maskell J. P. 1981; The pathogenicity of Bacteroides fragilis and related species estimated by intracutaneous infection in the guinea-pig. Journal of Medical Microbiology 14:131–140
    [Google Scholar]
  12. Melly M. A., Duke L. J., Liau D. F., Hash J. H. 1974; Biological properties of the encapsulated Staphylococcus aureus M. Infection and Immunity 10:389–397
    [Google Scholar]
  13. Mizuta K., Ohta M., Mori M., Hasegawa T., Nakashima I., Kato N. 1983; Virulence for mice of Klebsiella strains belonging to the Ol-group: relationship to their capsular (K) types. Infection and Immunity 40:56–61
    [Google Scholar]
  14. Reynard A. M., Beck M. E. 1976; Plasmid-mediated resistance to the bactericidal effects of normal rabbit serum. Infection and Immunity 14:848–850
    [Google Scholar]
  15. Rottini G., Dri P., Soranzo M. R., Patriarca P. 1975; Correlation between phagocytic activity and metabolic response of polymorphonuclear leukocytes toward different strains of Escherichia coli. Infection and Immunity 11:417–423
    [Google Scholar]
  16. Simoons-Smit A. M., Verweij-van Vught A. M. J. J., Kanis I. Y. R., MacLaren D. M. 1984; Virulence of Klebsiella strains in experimentally induced skin lesions in the mouse. Journal of Medical Microbiology 17:67–77
    [Google Scholar]
  17. Simoons-Smit A. M., Verweij-van Vught A. M. J. J., Kanis I. Y. R., MacLaren D. M. 1985; Chemiluminescence of human leukocytes stimulated by clinical isolates of Klebsiella. Journal of Medical Microbiology 19:333–338
    [Google Scholar]
  18. Toenniessen E. 1914; Ueber Vererbung und Variabilitåt bei Bakterien mit besonderer Beriicksichtigung der Virulenz. Zentralblatt fiir Bakteriologie I. Abt. Orig. 73:241–277
    [Google Scholar]
  19. Van Dijk W. C., Verbrugh H. A., Peters R., Van der Toi M. E., Peterson P. K., Verhoef J. 1979; Escherichia coli K antigen in relation to serum-induced lysis and phagocytosis. Journal of Medical Microbiology 12:123–130
    [Google Scholar]
  20. Van Dijk W. C., Verbrugh H. A., Van Erne-van der Toi M. E., Peters R., Verhoef J. 1981; Escherichia coli antibodies in opsonisation and protection against infection. Journal of Medical M icrobiology 14:381–389
    [Google Scholar]
  21. Verweij-van Vught A. M. J. J., Van den Bosch J. F., Namavar F., Sparrius M., MacLaren D. M. 1983; K antigens of Escherichia coli and virulence in urinary-tract infectiomstudies in a mouse model. Journal of Medical Microbiology 16:147–155
    [Google Scholar]
  22. Verweij-van Vught A. M. J. J., Namavar F., Peerbooms P. G. H., Sparrius M., MacLaren D. M. 1984; The role of different K antigens of Escherichia coli in phagocytosis by polymorphonuclear leukocytes. Journal of Medical Microbiology 17:141–150
    [Google Scholar]
  23. Welch W. D., Stevens P. 1979; Serum requirements necessary for the opsonophagocytosis of Escherichia coli 073:K92:H,. Current Microbiology 2:245–250
    [Google Scholar]
  24. Welch W. D., Martin W. J., Stevens P., Young L. S. 1979; Relative opsonic and protective activities of antibodies against K., O and lipid A antigens of Escherichia coli. Scandinavian Journal of Infectious Disease 11:291–301
    [Google Scholar]
  25. Williams P., Lambert P. A., Brown M. R. W., Jones R. J. 1983; The role of the O and K antigens in determining the resistance of Klebsiella aerogenes to serum killing and phagocytosis. Journal of General Microbiology 129:2181–2191
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-21-2-133
Loading
/content/journal/jmm/10.1099/00222615-21-2-133
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error