1887

Abstract

Summary

The adherence properties of non-fimbriate entero-invasive O124 in the guinea pig intestinal tract were studied. Quantitative in-vitro determinations were done by incubating radiolabelled bacteria with suspensions of viable intestinal cells released by treating loops of the guinea pig intestine with solutions containing EDTA, dithiothreitol and citrate. Non-bound bacteria were separated from the intestinal cells on a Percoll gradient. Only cells released from the colon, especially from its transverse and descending regions, avidly adhered to O124 (68−79 bacteria/cell), whereas the attachment to ileal cells was negligible. The adherence process was Ca and temperature-dependent, had an optimal H of 6.2 and was inhibited by fucose, glucose or mannose. Several pretreatment studies of the bacteria or the colonic cells showed that the adherence was mediated by a carbohydrate-binding protein (adhesin or lectin) on the colonic cells and not on the bacterial surface. Results of studies of in-vitro adherence to intestinal loops and to intact intestinal surfaces correlated well with the in-vitro findings. These results indicate that the adherence of entero-invasive O124 to the gut is similar to the attachment of and is quite different from that of enterotoxigenic .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-21-2-117
1986-03-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jmm/21/2/medmicro-21-2-117.html?itemId=/content/journal/jmm/10.1099/00222615-21-2-117&mimeType=html&fmt=ahah

References

  1. Ashkenazi S., Mirelman D. 1984; The effect of postnatal age on the adherence of Shigella flexneri, Escherichia coli 0124 and Escherichia coli 0128 to guinea pig intestinal cells. Pediatric Research 18:1366–1371
    [Google Scholar]
  2. Back E., Möllby R., Kaijser B., Stintzing G., Wadström T., Habte D. 1980; Enterotoxigenic Escherichia coli and other gramnegative bacteria of infantile diarrhea: surface antigens, hemagglutinins, colonization factor antigen and loss of enterotoxigenicity. Journal of Infectious Diseases 142:318–327
    [Google Scholar]
  3. Boedeker E. C. 1982; Enterocyte adherence of Escherichia coli: its relation to diarrheal disease. Gastroenterology 83:489–492
    [Google Scholar]
  4. Braun V. 1975; Covalent lipoprotein from the outer membrane of Escherichia coli. Biochimia et Biophysica Acta 415:335–377
    [Google Scholar]
  5. Cantey J. R., O’Hanley P. D., Blake R. K. 1977; A rabbit model of diarrhea due to invasive Escherichia coli. Journal of Infectious Diseases 136:640–648
    [Google Scholar]
  6. Cheney C. P., Schad P. A., Formal S. B., Boedeker E. C. 1980; Species specificity of in vitro Escherichia coli adherence to host intestinal cell membranes and its correlation with in vivo colonization and infectivity. Infection and Immunity 28:1019–1027
    [Google Scholar]
  7. Deneke C. F., Thorne G. M., Gorbach S. L. 1979; Attachment pili from enterotoxigenic Escherichia coli pathogenic for humans. Infection and Immunity 26:362–368
    [Google Scholar]
  8. Deneke C. F., McGowan K., Thorne G. M., Gorbach S. L. 1983; Attachment of enterotoxigenic Escherichia coli to human intestinal cells. Infection and Immunity 39:1102–1106
    [Google Scholar]
  9. Drachman R. H. 1974; Acute infectious gastroenteritis. Pediatric Clinics of North America 21:711–737
    [Google Scholar]
  10. Evans D. G., Evans D. J. 1978; New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups 06 and 08. Infection and Immunity 21:638–647
    [Google Scholar]
  11. Evans D. G., Evans D. J., Clegg S., Pauley J. A. 1979; Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infection and Immunity 25:738–748
    [Google Scholar]
  12. Gaastra W., deGraaf F. K. 1982; Host-specific fimbrial adhesins of non-invasive enterotoxigenic Escherichia coli strains. Microbiological Reviews 46:129–161
    [Google Scholar]
  13. Guerrant R. L., Moore R. A., Kirschenfeld P. M., Sandle M. A. 1975; Role of toxigenic and invasive bacteria in acute diarrhea of childhood. New England Journal of Medicine 293:567–573
    [Google Scholar]
  14. Hartley C. L., Neumann C. S., Richmond M. H. 1979; Adhesion of commensal bacteria to the large intestine wall in humans. Infection and Immunity 23:128–132
    [Google Scholar]
  15. Isaacson R. E. 1977; K99 surface antigen of Escherichia coli: purification and partial characterization. Infection and Immunity 15:272–279
    [Google Scholar]
  16. Izhar M., Nuchamowitz Y., Mirelman D. 1982; Adherence of Shigella flexneri to guinea pig intestinal cells is mediated by a mucosal adhesin. Infection and Immunity 35:1110–1118
    [Google Scholar]
  17. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurements with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  18. Mackowiak P. A. 1982; The normal microbial flora. New England Journal of Medicine 307:83–93
    [Google Scholar]
  19. Mirelman D., Altmann G., Eshdat Y. 1980; Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts. Journal of Clinical Microbiology 11:328–331
    [Google Scholar]
  20. Mirelman D., Nuchamowitz Y., Izhar M. Interactions between intestinal cells and shigellae. Journal of Cellular Biochemistry (in press).
    [Google Scholar]
  21. Mooi F. R., de Graaf F. K. 1979; Isolation and characterization of K88 antigens. FEMS Microbiology Letters 5:17–20
    [Google Scholar]
  22. Nandadasa H. G., Sargent G. F., Brown M. G. M., McNeish A. S., Williams P. H. 1981; The role of plasmids in adherence of invasive Escherichia coli to mammalian cells. Journal of Infectious Diseases 143:286–290
    [Google Scholar]
  23. Rothbaum R., McAdams A. J., Gianella R., Partin J. C. 1982; A clinicopathologic study of enterocyte-adherent Escherichia coli: a cause of protracted diarrhea in infants. Gastroenterology 83:441–454
    [Google Scholar]
  24. Sack R. B. 1980; Enterotoxigenic Escherichia coli: identification and characterization. Journal of Infectious Diseases 142:279–286
    [Google Scholar]
  25. Sereney B. 1957; Experimental keratoconjunctivitis Shigellosa. Acta Microbiologica Academiale Scientiorum Hungaricae 4:367–376
    [Google Scholar]
  26. Silva R. M., Toledo M. R. F., Trabulsi L. R. 1982; Correlation of invasiveness with plasmid in enteroinvasive strains of Escherichia coli. Journal of Infectious Diseases 146:706
    [Google Scholar]
  27. Ulshen M. H., Rollo J. L. 1980; Pathogenesis of Escherichia coli gastroenteritis in man—another mechanism. New England Journal of Medicine 302:99–101
    [Google Scholar]
  28. Weiser M. M. 1973; Intestinal epithelial cell surface membrane glycoprotein synthesis. 1. An indicator of cellular differentiation. Journal of Biological Chemistry 248:2536–2541
    [Google Scholar]
  29. Westphal O., Jann K. 1965 Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. In Whistler R. L. (ed) Methods of carbohydrate chemistry 5 Academic Press; New York: pp 83–91
    [Google Scholar]
  30. Williams P. H., Knutton S., Brown M. G. M., Candy D. C. A., McNeish A. S. 1984; Characterization of nonfimbrial mannose-resistant protein hemagglutinins of two Escherichia coli strains isolated from infants with enteritis. Infection and Immunity 44:592–598
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-21-2-117
Loading
/content/journal/jmm/10.1099/00222615-21-2-117
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error