1887

Abstract

Summary

The fungicidal activity of murine broncho-alveolar macrophages (BAM) against the yeast form of a virulent strain of was studied in the small-volume wells of a Terasaki plate. In a 4-h fungicidal assay, significant killing (16–33%) of the fungus by unstimulated BAM was demonstrated with BAM from normal mice and from mice rendered immune to lethal infection with . No significant differences between the activity of BAM from these two sources could be identified. Addition of 10% autologous normal or immune serum did not augment the macrophage fungicidal activity. Simultaneous experiments with peritoneal macrophages (PM) also gave reproducible killing of the yeast form in the wells of the Terasaki plate, but in the larger wells of microtitration plates, PM showed no significant fungicidal activity. On the other hand, BAM had similar fungicidal activity against in Terasaki and microtitration-plate wells. The modest fungicidal activity of BAM from immune mice against suggests that the resistance of immune mice to respiratory challenge is likely to be based on some augmentation of this first line of defence.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-21-1-7
1986-02-01
2020-01-28
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-21-1-7
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error