A disk-diffusion method for identification of yeasts was developed that depended on their different but distinct susceptibilities to the following chemicals: janus green, ethidium bromide, 2, 3, 5-triphenyltetrazolium chloride, brilliant green, cycloheximide and rhodamine 6G. For 594 of 623 routinely isolated yeasts, the disk-diffusion and the commercial API 20C auxanogram tests gave the same identification, an agreement of 95·3%. Only 8 of 1052 isolates from clinical specimens were not identified by the disk-diffusion method. The method is simple, inexpensive and technically straightforward and for most isolates gives an identification in 24 h.
ChoS. Y.,
ChoiH. Y.1979; Opportunistic fungal infection among cancer patients. A ten-yea autopsy study. American Journal of Clinical Pathology 72:617–621
EssersL.1982; Simple identification of anaerobic bacteria to genus level using typical antibiotic susceptibility patterns. Journal of Applied Bacteriology 52:319–323
FinegoldS. M.,
HaradaN. E.,
MillerL. G.1967; Antibiotic susceptibility patterns as aids in classification and characterization of gram-negative anaerobic bacilli. Journal of Bacteriology 94:1443–1450
MeinhofW.1972 Genitale Hefemykosen unter Behandlung mit oralen Kontrazeptiva.. In
ZaunH.
(ed) Ovulations-hemmer in der Dermatologie, G. Thieme-Verlag; Stuttgardt:
SobczakH.1981; Janusgrun-Sensibilität als Differenzierungskriterium für Candida parapsilosis, Torulopsis glabrata und Torulopsis Candida. Mykosen 24:731–742