1887

Abstract

Summary

An immunodiagnostic test for the detection of a soluble nonprotein mycobacterial antigen by reverse passive haemagglutination with IgM murine monoclonal antibody was developed. The test was used to analyse the cerebrospinal fluid of 89 patients with tuberculous meningitis (TBM) from India and 127 control subjects from India and the UK. The antigen was demonstrable in 88% of culture-positive and 73% of culture-negative TBM patients. However, it was also detected in 21% of Indian patients with pyogenic meningitis, and in 8% of Indian and 1% of UK control subjects. Antibodies binding to a soluble mycobacterial extract were detected at low titre in 68% of all subjects with TBM and in 37% of Indian cases of pyogenic meningitis. Because patients with TBM had raised levels of the antigen and of antibodies to the antigen, the possible role of immune complexes in the pathogenesis of the disease is briefly discussed.

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/content/journal/jmm/10.1099/00222615-20-2-239
1985-10-01
2022-10-06
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References

  1. Carr R. I. et al. 1980; Immune complexes and antibodies to BCG in sera from patients with mycobacterial infections. Clinical and Experimental Immunology 39:562–569
    [Google Scholar]
  2. Hewitt J., Coates ARM., Mitchison D. A., Ivanyi J. 1982; The use of murine monoclonal antibodies without purification of antigen in the serodiagnosis of tuberculosis. Journal of Immunological Methods 55:205–211
    [Google Scholar]
  3. Ivanyi J., Krambovitis E., Keen M. 1983; Evaluation of a monoclonal antibody (TB72) based serological test for tuberculosis. Clinical and Experimental Immunology 54:337–345
    [Google Scholar]
  4. Ivanyi J., Sinha S., Aston R., Cussell D., Keen M., Sengupta U. 1983; Definition of species specific and cross-reactive antigenic determinants of Mycobacterium leprae using monoclonal antibodies. Clinical and Experimental Immunology 52:528–536
    [Google Scholar]
  5. Ivanyi J., Morris J. A., Keen M. Studies with monoclonal antibodies to mycobacteria. In Macario AC., Macario AJL. eds Monoclonal antibodies against bacteria. Academic Press; New York: in press
    [Google Scholar]
  6. Kennedy D. H., Fallon R. J. 1979; Tuberculous meningitis. Journal of the American Medical Association 241:264–268
    [Google Scholar]
  7. Kinnman J., Fryden A., Eriksson S., Moller E., Link H. 1981; Tuberculous meningitis: immune reactions within the central nervous system. Scandinavian Journal of Immunology 13:289–296
    [Google Scholar]
  8. Kuo C. T. 1969; The diagnosis of tuberculous meningitis by immunologic reaction of cerebrospinal fluid. American Review of Respiratory Diseases 100:565–568
    [Google Scholar]
  9. Minden P., McClatchy J. K., Cooper R., Bardana E. J., Farr R. S. 1972; Shared antigens between mycobacterium bovis (BCG) and other bacterial species. Science 176:57–58
    [Google Scholar]
  10. Munoz O., Guiscafre H., Feria A., Lopez M., Martinez-Cairo S. 1978; Indirect hemagglutination with PPD in cerebrospinal fluid for the tuberculous meningoencephalitis. Archivos de Investigacion Medica (Mexico) 9:469–476
    [Google Scholar]
  11. Praputpittaya K., Ivanyi J. 1985; Detection of an antigen (MY4) common to M. tuberculosis and M. leprae by ‘tandem’ immunoassay. Journal of Immunological Methods 79:149–157
    [Google Scholar]
  12. Sada E., Ruiz-Palacios G. M., Lopez-Vidal Y., Ponce de Leon S. 1983; Detection of mycobacterial antigens in cerebrospinal fluid of patients with tuberculous meningitis by enzyme-linked immunosorbent assay. Lancet 2:651–652
    [Google Scholar]
  13. Scott M. L., Thornley M. J., Coombs R R A., Bradwell A. R. 1981; Measurement of human serum IgE and IgA by reverse passive antiglobulin haemagglutination. International Archives of Allergy and Applied Immunology 64:222–229
    [Google Scholar]
  14. Udani P. M. 1980; Tuberculosis in children with special reference to neurotuberculosis. Annals of the National Academy of Medical Sciences (India) 16:121–161
    [Google Scholar]
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