@article{mbs:/content/journal/jmm/10.1099/00222615-20-2-157, author = "Coleman, D. C. and Pomeroy, H. and Estridge, J. K. and Keane, C. T. and Cafferkey, M. T. and Hone, R. and Foster, T. J.", title = "Susceptibility to Antimicrobial Agents and Analysis of Plasmids in Gentamicin- and Methicillin-Resistant Staphylococcus Aureus From Dublin Hospitals", journal= "Journal of Medical Microbiology", year = "1985", volume = "20", number = "2", pages = "157-167", doi = "https://doi.org/10.1099/00222615-20-2-157", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-20-2-157", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Summary Methicillin- and gentamicin-resistant Staphylococcus aureus (MGRSA) strains isolated from Dublin Hospitals were classified into two groups (phenotypes). Phenotype-I strains expressed high level resistance to gentamicin and were susceptible to fusidic acid; strains resistant to tetracycline harboured a 3 x 106-mol. wt plasmid. Strains in phenotype II usually expressed low level resistance to gentamicin, were resistant to fusidic acid and often harboured a (22-24) x 106-mol. wt plasmid that specified resistance to ethidium bromide, tetracycline, kanamycin, neomycin and trimethoprim, or to combinations of these markers. A few phenotype-II strains expressed higher levels of resistance to gentamicin and other aminoglycosides. All MGRSA strains carried a 21 x 106-mol. wt plasmid conferring resistance to penicillin, ethidium bromide, cadmium and mercury. Gentamicin resistance was invariably chromosomal and all strains carried chromosomal resistance to methicillin, erythromycin, streptomycin and spectinomycin. Several methicillin-resistant S. aureus (MRSA) strains isolated before the emergence of gentamicin resistance harboured a 21 x 106-mol. wt penicillinase plasmid with the same restriction endonuclease profile as that from some MGRSA strains. Some MRSA strains carried other plasmids related to those found in MGRSA strains.", }