1887

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Volume 20, Issue 2

Susceptibility to Antimicrobial Agents and Analysis of Plasmids in Gentamicin- and Methicillin-Resistant From Dublin Hospitals Free

Abstract

Summary

Methicillin- and gentamicin-resistant (MGRSA) strains isolated from Dublin Hospitals were classified into two groups (phenotypes). Phenotype-I strains expressed high level resistance to gentamicin and were susceptible to fusidic acid; strains resistant to tetracycline harboured a 3 x 10-mol. wt plasmid. Strains in phenotype II usually expressed low level resistance to gentamicin, were resistant to fusidic acid and often harboured a (22-24) x 10-mol. wt plasmid that specified resistance to ethidium bromide, tetracycline, kanamycin, neomycin and trimethoprim, or to combinations of these markers. A few phenotype-II strains expressed higher levels of resistance to gentamicin and other aminoglycosides. All MGRSA strains carried a 21 x 10-mol. wt plasmid conferring resistance to penicillin, ethidium bromide, cadmium and mercury. Gentamicin resistance was invariably chromosomal and all strains carried chromosomal resistance to methicillin, erythromycin, streptomycin and spectinomycin.

Several methicillin-resistant (MRSA) strains isolated before the emergence of gentamicin resistance harboured a 21 x 10-mol. wt penicillinase plasmid with the same restriction endonuclease profile as that from some MGRSA strains. Some MRSA strains carried other plasmids related to those found in MGRSA strains.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology, 1985
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1985-10-01
2024-04-19
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Susceptibility to Antimicrobial Agents and Analysis of Plasmids in Gentamicin- and Methicillin-Resistant Staphylococcus Aureus From Dublin Hospitals
J. Med. Microbiol. 20, 157 (1985); https://doi.org/10.1099/00222615-20-2-157
/content/journal/jmm/10.1099/00222615-20-2-157
/content/journal/jmm/10.1099/00222615-20-2-157
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