1887

Abstract

SUMMARY

Yields of reovirus type 3 were depressed and cytopathic changes delayed when calf or sheep serum was present inthe culture medium. Both sera also neutralised free reovirus. Fractionation of pooled serum samples by DEAE- cellulose and Sephadex G-200 chromatography showed that in thecase of calf serum the inhibitory and neutralising activities were associated with the γG globulin fractions. Therefore it is likely that the inhibitory activity ofcalf serum is due entirely to the presence of neutralising antibody. In the case of sheep serum, however, neutralising and inhibitory activities were dissociated. Virus-neutralising activity in this case was maximal in the γG globulin fractions, whilst the inhibitory activityresided in the γM fraction. The inhibitory componentof sheep serum appears to act by blocking virus release.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-2-2-117
1969-05-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/2/2/medmicro-2-2-117.html?itemId=/content/journal/jmm/10.1099/00222615-2-2-117&mimeType=html&fmt=ahah

References

  1. Allen R., Finkelstein R. A., Sulkin S. E. 1958 Tex. Rep. Biol. Med 16:391
    [Google Scholar]
  2. Bügel K. 1966 Zentbl. VetMed B 13:636
    [Google Scholar]
  3. Flodin P., Killander J. 1962 Biochim. biophys. Acta 63:403
    [Google Scholar]
  4. Ginsberg H. S. 1960 Bad. Rev 24:141
    [Google Scholar]
  5. Gomatos P. J., Tamm I., Dales S., Franklin R. M. 1962 Virology 17:441
    [Google Scholar]
  6. Lamont P. H. 1966 Proc. Roy. Soc. Med 59:50
    [Google Scholar]
  7. Levy H. B., Sober H. A. 1960 Proc. Soc. Exp. Biol. Med 103:250
    [Google Scholar]
  8. Mcferran J. B. 1962 J. Path. Bad 83:73
    [Google Scholar]
  9. Mcferran J. B., Dane D. S., Briggs E. M., Connor T., Nelson R. 1968 Ibid 95:93
    [Google Scholar]
  10. Rosen L., Abinanti F. R., Hovis JANET F. 1963 Amer. J. Hyg 77:38
    [Google Scholar]
  11. Stanley N. F. 1967 Br. Med. Bull 23:150
    [Google Scholar]
  12. Svehag S. E. 1964 J. Exp. Med 119:517
    [Google Scholar]
  13. Takemori N., Nomura S., Nakano M., Morioka Y., Henmi M., Kitaoka M. 1957 Science, N. Y 125:1196
    [Google Scholar]
  14. Takemori N., Nomura S., Nakano M., Morioka Y., Henmi M., Kitaoka M. 1958 Virology 5:30
    [Google Scholar]
  15. Wallis C., Melnick J. L., Bianchi M. 1962 Tex. Rep. Biol. Med 20:693
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-2-2-117
Loading
/content/journal/jmm/10.1099/00222615-2-2-117
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error