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Six of 11 test strains of Yersinia enterocolitica and related species that carried other markers of pathogenicity were found to associate with Henle 407 epithelial cells in vitro. All Henle-positive strains were hydrophobic when tested by hydrophobic interaction chromatography with phenyl-Sepharose and by partitioning in an aqueous-hexadecane mixture. Hydrophobicity was also exhibited by some of the Henle-negative strains. None of the test strains aggregated in low concentrations of ammonium sulphate, suggesting that protein structures such as fimbriae were not involved in hydrophobicity or epithelial cell association.
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