When grown in specified conditions and tested by a rocked-tile method, 40 of 41 isolates of two species of formed simultaneously at least two haemagglutinins among which were: (i) a mannose-sensitive haemagglutinin with strongest activity for guinea-pig or fowl red cells, formed by all of 31 isolates of and 9 of 10 isolates of ss. ; (ii) a haemagglutinin, sensitive to L-fucose or D-mannose, that reacted with human red cells and which was formed by all 41 isolates; and (iii) a mannose-resistant ‘tanned red cell’ haemagglutinin formed by 29 isolates of and one isolate of ss. . Results emphasise that for the fullest possible identification of haemagglutinins produced by ., strains should be cultured in a variety of conditions and tested with a wide range of red-cell species.


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