1887

Abstract

Summary

The virulence of in gnotobiotic piglets was studied by intranasal infection with 11 cultures derived from eight strains isolated from pigs (4), dogs (2), a human subject and a monkey. Six of the cultures contained organisms in phase I and five contained phenotypically different phase-III or -IV organisms. Of the phase-III and -IV cultures, four were derived from strains that had been isolated in phase I. Colonisation of the nasal cavity was investigated by counting bacteria in nasal swabs and washings. The toxigenicity of cell extracts from each strain and variant was determined by tests of lethality in mice or of cytopathogenicity in cell cultures. The results showed that two phase-I cultures from pigs colonised the nasal cavity and respiratory tract of gnotobiotic piglets better than did four phase-I cultures from other species. Phase-I organisms invariably produced capsules, fimbriae and mannose-resistant haemagglutination of guinea-pig erythrocytes. Four of five cultures in phases III and IV consisted of organisms that did not produce capsules, fimbriae or haemagglutination and colonised the nasal cavity poorly. Phase variation from I to III occurred in culture and , but variation from III to I occurred only and was accompanied by enhanced colonisation. Gnotobiotic piglets infected with porcine phase-I organisms exhibited atrophy of the nasal turbinate bones after 28 days; these organisms produced significantly more toxin than did bacteria in phase I from other species, or those in phases III and IV. It was concluded that the development of turbinate atrophy was associated with (1) the ability to produce heavy, persistent colonisation in the nasal cavity, and (2) the production of a heat-labile toxin. Only the two porcine phase-I cultures possessed both properties.

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1985-04-01
2024-04-24
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References

  1. Brassinne M, Dewaele A, Gouffaux M. 1976; Intranasal infection with Bordetella bronchiseptica in gnotobiotic piglets. Research in Veterinary Science 20:162–166
    [Google Scholar]
  2. Collings L A. 1983; Pathogenicity of Bordetella bronchiseptica in porcine atrophic rhinitis. Ph.D. Thesis University of Reading;
    [Google Scholar]
  3. Done J T, Richardson M D, Hebert C N. 1964; Rhinitis of swine: A survey carried out in Britain in 1956-57. In: Animal Disease Survey No. 3 H.M. Stationery Office; London:29
    [Google Scholar]
  4. Duguid J P, Gillies R R. 1957; Fimbriae and adhesive properies in dysentery bacilli. Journal of Pathology and Bacteriology 74:397–411
    [Google Scholar]
  5. Ehresmann B, Imbauli P, Weil J H. 1973; Spectrophotometric determination of protein concentration in cell extracts containing tRNA’s and rRNA’s. Analytical Biochemistry 54:454–463
    [Google Scholar]
  6. Endoh M, Takezawa T, Nakase Y. 1980; Adenylate cyclase activity of Bordetella organisms. Its production in liquid medium. Microbiology and Immunology 24:95–104
    [Google Scholar]
  7. Ezzell J W, Dobrogosz W J, Kloos W E, Manclark C R. 1981; Phase-shift markers in Bordetella: alterations in envelope proteins. Journal of Infectious Diseases 143:562–569
    [Google Scholar]
  8. Giles C J, Smith I M, Baskerville A J, Brothwell E. 1980; Clinical, bacteriological and epidemiological observations on infectious atrophic rhinitis of pigs in Southern England. Veterinary Record 106:25–28
    [Google Scholar]
  9. Hanada M, Shimoda K, Tornita S, Nakase Y, Nishiyama Y. 1979; Production of lesions similar to naturally occurring swine atrophic rhinitis by cell-free sonicated extract of Bordetella bronchiseptica . Japanese Journal of Veterinary Science 41:1–8
    [Google Scholar]
  10. Jenkins E M. 1978; An agglutination test for the detection of Bordetella bronchiseptica infection in swine. Canadian Journal of Comparative Medicine and Veterinary Science 42:286–292
    [Google Scholar]
  11. Jones G W, Rutter J M. 1974; The association of K88 antigen with haemagglutinating activity in porcine strains of Escherichia coli . Journal of General Microbiology 84:135–144
    [Google Scholar]
  12. Kemeny L J. 1972; Experimental atrophic rhinitis produced by a Bordetella bronchiseptica culture in young pigs. Cornell Veterinarian 62:477–485
    [Google Scholar]
  13. Lawson G M. 1940; Modified technique for staining capsules of Haemophiluspertussis . Journal of Laboratory and Clinical Medicine 25:435–438
    [Google Scholar]
  14. Leslie P H, Gardner A D. 1931; The phases of Haemophilus pertussis . Journal of Hygiene 31:423–434
    [Google Scholar]
  15. Miniats O P, Johnson J A. 1980; Experimental atrophic rhinitis in gnotobiotic pigs. Canadian Journal of Comparative Medicine and Veterinary Science 44:358–365
    [Google Scholar]
  16. Morse S I. 1976; Biologically active components and properties of Bordetella pertussis . Advances in Applied Microbiology 20:9–26
    [Google Scholar]
  17. Munoz J. 1971; Protein toxins from Bordetella pertussis . Kadis S, Montie T C, Ajl S J. Microbial toxins 2A Academic Press; New York:271–300
    [Google Scholar]
  18. Nakase Y. 1957a; Studies on Haemophilus bronchisepticus 1. The antigenic structures of H bronchisepticus from a guinea pig. Kitasato Archives of Experimental Medicine 30:57–72
    [Google Scholar]
  19. Nakase Y. 1957b; Studies on Haemophilus bronchisepticus II. Phase variation of H bronchisepticus . Kitasato Archives of Experimental Medicine 30:73–78
    [Google Scholar]
  20. Ross R F, Duncan J R, Switzer W P. 1963; Turbinate atrophy in swine produced by pure cultures of Bordetella bronchiseptica . Veterinary Medicine 58:566–570
    [Google Scholar]
  21. Ross R F, Switzer W P, Duncan J P. 1967; Comparison of pathogenicity of various isolates of Bordetella bronchiseptica in young pigs. Canadian Journal of Comparative Medicine and Veterinary Science 31:53–57
    [Google Scholar]
  22. Rothamsted Experimental Station Department of Statistics; 1977 Genstat: a general statistical program Rothamsted Experimental Station; Harpenden, Herts:
    [Google Scholar]
  23. Rutter J M. 1981; Quantative observations on Bordetella bronchiseptica infection in atrophic rhinitis of pigs. Veterinary Record 108:451–454
    [Google Scholar]
  24. Rutter J M. 1983; Virulence of Pasteurella multocida in atrophic rhinitis of gnotobiotic pigs infected with Bordetella bronchiseptica . Research in Veterinary Science 34:287–295
    [Google Scholar]
  25. Rutter J M, Francis L M A, Sansom B F. 1982; Virulence of Bordetella bronchiseptica from pigs with or without atrophic rhinitis. Journal of Medical Microbiology 15:105–116
    [Google Scholar]
  26. Rutter J M, Luther P D. 1984; A cell culture assay for toxigenic Pasteurella multocida from atrophic rhinitis of pigs. Veterinary Record 114:393–396
    [Google Scholar]
  27. Shimizu T, Nakagawa M, Shibata S, Suzuki K. 1971; Atrophic rhinitis produced by intranasal inoculation of Bordetella bronchiseptica in hysterotomy produced, colostrum-deprived pigs. Cornell Veterinarian 61:696–705
    [Google Scholar]
  28. Skelly B J, Pruss M, Pellegrino R, Andersen D, Abruzzo G. 1980; Variation in degree of atrophic rhinitis with field isolates ofBordetella bronchiseptica . Proceedings of the 6 th Congress of the International Pig Veterinary Societyheld in Copenhagen210
    [Google Scholar]
  29. Switzer W P. 1981; Bordetellosis. Leman AD. Diseases of Swine, 5. Iowa State University Press; Ames, Iowa, USA:497–507
    [Google Scholar]
  30. Wardlaw A C, Parton R, Hooker M J. 1976; Loss of protective antigen, -histamine-sensitising factor and envelope polypeptides in cultural variants of Bordetella pertussis . Journal of Medical Microbiology 9:89–100
    [Google Scholar]
  31. Weiss A A, Falkow S. 1984; Genetic analysis of phase change in Bordetellapertussis . Infection and Immunity 43:263–269
    [Google Scholar]
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