1887

Abstract

Summary

Different colonial morphologies were found among colonies of strain 2996 (B: 2b: P1.2). Examination of cultures, selected on the basis of colony transparency or opacity, revealed that both lipopolysaccharides (LPS) and class-5 outer membrane proteins (OMP) are associated with differences in colonial morphology. Among 13 variants, four LPS variants and two class-5 OMP variants were recognised. All variants were non-fimbriate. The LPS variations were confirmed by immunoprecipitation. In addition to these qualitative variations of LPS, meningococci synthesise LPS of different molecular size depending upon growth phase; larger LPS molecules were found after analysis of stationary-phase cultures than with exponential-phase cultures. These changes did not cause a change in serotyping characteristics. The recognition in this study of intra-strain heterogeneity of meningococcal LPS and class-5 OMPs is important for the understanding of meningococcal pathogenicity. This heterogeneity was also detected in simultaneous isolates from different sites of a patient.

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1985-04-01
2022-01-21
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References

  1. Andersen B M, Solberg O. 1984; Endotoxin liberation and invasiveness of Neisseria meningitidis. Scandinavian Journal of Infectious Diseases, in press
    [Google Scholar]
  2. Ashton F E, Ryan J A, Jones C, Brodeur B R, Diena B B. 1983; Serotypes of Neisseria meningitidis associated with an increased incidence of meningitis cases in the Hamilton area, Ontario, during 1978 and 1979. Canadian Journal of Microbiology 29:129–136
    [Google Scholar]
  3. Austrian R. 1953; Morphologic variation in pneumococcus. 1. An analysis of the bases for morphologic variation in pneumococcus and description of a hitherto undefined morphologic variant. Journal of Experimental Medicine 98:21–34
    [Google Scholar]
  4. DeVoe I W, Gilchrist J E. 1978; Pillation and colonial morphology among laboratory strains of meningococci. Journal of Clinical Microbiology 7:379–384
    [Google Scholar]
  5. Frasch C E, Zollinger W D, Poolman J T. Serotyping of Neisseria meningitidis and a proposed scheme for designation of serotypes. Reviews of Infectious Diseases, in press
    [Google Scholar]
  6. Frøholm L O, Jyssum K, Bøvre K. 1973; Electron microscopical and cultural features of Neisseria meningitidis competence variants. Acta Pathologica et Microbiologica Scandinavica Section B 81:525–537
    [Google Scholar]
  7. McGee Z A, Street C H, Chappell C L, Cousar E S, Morris F, Horn R G. 1979; Pili of Neisseria meningitidis: effect of media on maintenance of piliation, characteristics of pili, and colonial morphology. Infection and Immunity 24:194–201
    [Google Scholar]
  8. Nikaido H, Nikaido K, Subbaiah T V, Stocker B A D. 1964; Rough mutants of Salmonella typhimurium 3. Enzymatic synthesis of nucleolide-sugar compounds. Nature 201:1298–1302
    [Google Scholar]
  9. Poolman J T, De Marie S, Zanen H C. 1980a; Variability of low-molecular-weight, heat-modifiable outer membrane proteins of Neisseria meningitidis. Infection and Immunity 30:642–648
    [Google Scholar]
  10. Poolman J T, Hopman C T P, Zanen H C. 1980b; Immunochemical characterization of Neisseria meningitidis serotype antigens by immunodiffusion and SDS-polyacrylamide gel electro-phoresis immunoperoxidase techniques and the distribution of serotypes among cases and carriers. Journal of General Microbiology 116:465–473
    [Google Scholar]
  11. Poolman J T, Hopman C T P, Zanen H C. 1982; Problems in the definition of meningococcal serotypes. FEMS Microbiology Letters 13:339–348
    [Google Scholar]
  12. Poolman J T, Hopman C T P, Zanen H C. 1983; Immunogenicity of meningococcal antigens as detected in patient sera. Infection and Immunity 40:398–406
    [Google Scholar]
  13. Silverman M, Zieg J, Hilmen M, Simon M. 1979; Phase variation in Salmonella: genetic analysis of a recombinational switch. Proceedings of the National Academy of Sciences of the USA 76:391–395
    [Google Scholar]
  14. Stephens D S, McGee Z A. 1983; Association of virulence of Neisseria meningitidis with transparent colony type and low-molecular-weight outer membrane proteins. Journal of Infectious Diseases 147:282–292
    [Google Scholar]
  15. Swanson J. 1982; Colony opacity and protein II compositions of gonococci. Infection and Immunity 37:359–368
    [Google Scholar]
  16. Swanson J, Barrera O. 1983; Gonococcal pilus subunit size heterogeneity correlates with transitions in colony piliation phenotype, not with changes in colony opacity. Journal of Experimental Medicine 158:1459–1472
    [Google Scholar]
  17. Tsai C M, Frasch C E, Mocca L F. 1981; Five structural classes of major outer membrane proteins in Neisseria meningitidis. Journal of Bacteriology 146:69–78
    [Google Scholar]
  18. Tsai C M, Boykins R, Frasch C E. 1983; Heterogeneity and variation among Neisseria meningitidis lipopolysaccharides. Journal of Bacteriology 155:498–504
    [Google Scholar]
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