1887

Abstract

Summary

A model of the promixal colon was used to investigate the effects of H on fermentation by colonic bacteria . Twelve continuous anaerobic cultures of human faecal bacteria were maintained at constant H in a medium simulating ileostomy effluent. Five cultures were maintained at H 7, five at H 6, and two at H 5. The H of each of three further cultures was altered after they had reached steady state, either from 7 to 6 and then to 5, or from 5 to 6 to 7.

Both experimental designs showed that the H exerted an important effect on bacterial metabolism without causing major changes in bacterial populations. Osmolality was lower in cultures run at a low H. Total volatile fatty acid concentration was decreased at H 5, and the production of propionic acid rather than acetic acid was favoured at H 6. Changing the H had no significant influence on the production of ammonia in these systems.

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1985-04-01
2024-04-25
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References

  1. Binder H J, Filburn B, Floch M H. 1975; Bile acid inhibition of intestinal anaerobic organisms. American Journal of Clinical Nutrition 28:119–125
    [Google Scholar]
  2. Bown R L, Gibson J A, Sladen G E, Hicks B, Dawson A M. 1974; Effects of lactulose and other laxatives on ileal and colonie pH measured by a radiotelemetry device. Gut 15:999–1004
    [Google Scholar]
  3. Collee J G, Watt B, Fowler E B, Brown R. 1972; An evaluation of the Gas-pak system in the culture of anaerobic bacteria. Journal of Applied Bacteriology 35:71–82
    [Google Scholar]
  4. Cowan S T. 1974; Manual for the identification of medical bacteria. , 2. Cambridge University Press; Cambridge:
    [Google Scholar]
  5. Cummings J H. 1981; Short chain fatty acids in the human colon. Gut 22:763–779
    [Google Scholar]
  6. Down P F, Agostini L, Murison J, Wrong O M. 1972; The interrelations of faecal ammoniapH and bicarbonate: evidence of colonie absorption of ammonia by non-ionic diffusion. Clinical Science 43:101–114
    [Google Scholar]
  7. Hill A B. 1971; Principles of medical statistics. , 9. The Lancet Ltd London:149–150
    [Google Scholar]
  8. Holbrook W P, Ogston S A, Ross P W. 1978; A method for the isolation of Bacteroides melaninogenicus from the human mouth. Journal of Medical Microbiology 11:203–207
    [Google Scholar]
  9. McNeil N I, Cummings J H, James W P T. 1978; Short chain fatty acid absorption by the human large intestine. Gut 19:819–822
    [Google Scholar]
  10. Percy-Robb I W, Collee J G. 1972; Bile acids: ApH dependent antibacterial system in the gut?. British Medical Journal 3:813–815
    [Google Scholar]
  11. Perman J A, Modler S, Olson A C. 1981; Role of pH in production of hydrogen from carbohydrates by colonie bacterial flora. Journal of Clinical Investigation 67:643–650
    [Google Scholar]
  12. Roediger W E W. 1980; Role of anaerobic bacteria in the metabolic welfare of the colonie mucosa of man. Gut 21:793–798
    [Google Scholar]
  13. Rotimi V O, Faulkner J, Duerden B I. 1980; Rapid method for the identification of clinical isolates of gram-negative anaerobic bacilli. Medical Laboratory Science 37:331–339
    [Google Scholar]
  14. Rotimi V O, Duerden B I. 1981; The development of the bacterial flora in normal neonates. Journal of Medical Microbiology 14:51–62
    [Google Scholar]
  15. Ruppin H, Bar-meir S, Soergel K H, Wood C M, Schmitt M G. 1980; Absorption of short chain fatty acids by the colon. Gastroenterology 78:1500–1507
    [Google Scholar]
  16. Russell J B, Sharp W M, Baldwin R L. 1979; The effect ofpH on maximum bacterial growth rate and its possible role as a determinant of bacterial competition in the rumen. Journal of Animal Science 48:251–255
    [Google Scholar]
  17. Saunders D R, Wiggins H S. 1981; Conservation of mannitol, lactulose and raffinose by the human colon. American Journal of Physiology 241:397–402
    [Google Scholar]
  18. Slyter L L, Bryant M P, Wolin M J. 1966; Effect of pH on population and fermentation in a continuously cultured rumen ecosystem. Applied Microbiology 14:573–578
    [Google Scholar]
  19. Varley H, Gowenlock A H, Bell M. 1980; Practical clinical biochemistry. , 5. 1 William Heinemann Medical Books Ltd; London:457
    [Google Scholar]
  20. Veilleux B G, Rowland I. 1981; Simulation of the rat intestinal ecosystem using a two-stage continuous culture system. Journal of General Microbiology 123:103–115
    [Google Scholar]
  21. Vince A, Killingley M, Wrong O M. 1978; Effect of lactulose on ammonia production in a fecal incubation system. Gastroenterology 74:544–549
    [Google Scholar]
  22. Willis A T, Bullen C L, Williams K, Fagg C G, Bourne A, Vignon M. 1973; Breast milk substitute: a bacteriological study. British Medical Journal 4:67–72
    [Google Scholar]
  23. Willis A T. 1977; Anaerobic bacteriology. Clinical and laboratory practice, 3. Butter-worths; London:
    [Google Scholar]
  24. Wolin M J. 1981; Fermentation in the rumen and human large intestine. Science 2131463–1468
    [Google Scholar]
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