1887

Abstract

Summary

Phagocytosis by human leukocytes, phagosomal H and degradation of seven species of bacteria were studied by a flow cytometric method. The percentage of phagocytosing leukocytes was similar for all bacterial strains examined, but and were more slowly phagocytosed than other bacteria. The phagosomal H surrounding the different bacterial species 15 min after the start of phagocytosis were: 4·4; 4·9; 5·1; 5·2; 5·3; 5·4; and 5·7. For longer incubation periods, the phagosomal remained nearly constant. and were the most readily degraded of the species tested. The proteins of all bacteria were degraded more rapidly than their DNA as determined by measurements of the loss of fluorescein-isothiocyanate-fluorescence and ethidium bromide-fluorescence, respectively. The rate of degradation varied from one bacterial species to another. The degradation of proteins and DNA was maximal for bacteria residing in a phagosomal environment estimated to be between H 5·2 and 5·4.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-19-1-115
1985-02-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/19/1/medmicro-19-1-115.html?itemId=/content/journal/jmm/10.1099/00222615-19-1-115&mimeType=html&fmt=ahah

References

  1. Baggiolini M. 1972; The enzymes of the granules of polymorphonuclear leukocytes and their functions. Enzyme 13:132–160
    [Google Scholar]
  2. Barret A J. 1969; Properties of lysosomal enzymes. Dingle J T, Fell H B. Lysosomes in biology and pathology 2 North-Holland Publishing Co; Amsterdam:245–312
    [Google Scholar]
  3. Bassøe C-F. 1984; Processing of Staphylococcus aureus and zymosan particles by human leukocytes measured by flow cytometry. Cytometry 5:86–91
    [Google Scholar]
  4. Bassøe C-F, Solsvik J, Laerum O D. 1980; Quantitation of single cell phagocytic capacity by flow cytometry. Laerum O D, Thorud E, Lindmo T. Flow cytometry IV Universitetsforlaget; Bergen:170–174
    [Google Scholar]
  5. Bassøe C-F, Laerum O D, Glette J, Hopen G, Haneberg B, Solberg C O. 1983a; Simultaneous measurement of phagocytosis and phagosomal pH by flow cytometry: Role of polymorphonuclear neutrophilic leukocyte granules in phagosome acidification. Cytometry 4:254–262
    [Google Scholar]
  6. Bassøe C-F, Laerum O D, Solberg C O, Haneberg B. 1983b; Phagocytosis of bacteria by human leukocytes measured by flow cytometry. Proceedings of The Society for Experimental Biology and Medicine 174:182–186
    [Google Scholar]
  7. Bjerknes R, Bassøe C-F. 1983; Human leukocyte phagocytosis of zymosan particles measured by flow cytometry. Acta Pathologica et Microbiologica Scandinavica, Section C 91:341–348
    [Google Scholar]
  8. Cohn Z A. 1963; The fate of bacteria within phagocytic cells. I. The degradation of isotopically labelled bacteria by polymorphonuclear leukocytes and macrophages. Journal of Experimental Medicine 117:27–42
    [Google Scholar]
  9. Elsbach P. 1980; Degradation of microorganisms by phagocytic cells. Reviews of Infectious Diseases 2:106–128
    [Google Scholar]
  10. Elsbach P, Pettis P, Beckerdite S, Franson R. 1973; Effects of phagocytosis by rabbit granulocytes on macromolecular synthesis and degradation in different species of bacteria. Journal of Bacteriology 115:490–497
    [Google Scholar]
  11. Geisow M J, D’Arcy-Hart P, Young M R. 1981; Temporal changes of lysosome and phagosome pH during phagolysosome formation in macrophages: Studies by fluorescence spectro- scopy. Journal of Cell Biology 89:645–652
    [Google Scholar]
  12. Jacques Y V, Bainton D F. 1978; Changes in pH within the phagocytic vacuoles of human neutrophils and monocytes. Laboratory Investigation 39:179–185
    [Google Scholar]
  13. Jensen M S, Bainton D F. 1973; Temporal changes in pH within the phagocytic vacuole of the polymorphonuclear neutrophilic leukocyte. Journal of Cell Biology 56:379–388
    [Google Scholar]
  14. Klebanoff S J. 1975; Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes. Seminars in Hematology 12:117–142
    [Google Scholar]
  15. Klebanoff S J, Clark R A. 1978 The neutrophil: Function and clinical disorders North-Holland Publishing Co; Amsterdam:447–451
    [Google Scholar]
  16. Lamers M C, de Groot E R, Roos D. 1982; Phagocytosis and degradation of DNA-anti-DNA complexes by human phagocytes. Influence of the antibody class. Laboratory Investigation 46:393–399
    [Google Scholar]
  17. Mandell G L. 1970; Intraphagosomal pH of human polymorphonuclear neutrophils. Proceedings of the Society for Experimental Biology and Medicine 134:447–449
    [Google Scholar]
  18. Melamed M R, Adams L R, Zimring A, Murnick J G, Mayer K. 1972; Preliminary evaluation of acridine orange as a vital stain for automated differential leukocyte counts. American Journal of Clinical Pathology 57:95–102
    [Google Scholar]
  19. Mims C A. 1982; The pathogenesis of infectious disease. , 2. Academic Press; London:56–81
    [Google Scholar]
  20. Rous P. 1925; The relative reaction within living mammalian tissues. II. On the mobilization of acid material within cells, and the reaction as influenced by the cell state. Journal of Experimental Medicine 41:399–411
    [Google Scholar]
  21. Segal A W, Geisow M, Garcia R, Harper A, Miller R. 1981; The respiratory burst of phagocytic cells is associated with a rise in vacuolar pH. Nature 290:406–409
    [Google Scholar]
  22. Sprick M G. 1956; Phagocytosis of M. tuberculosis and M. smegmatis stained with indicator dyes. American Review of Tuberculosis and Pulmonary Diseases 74:552–565
    [Google Scholar]
  23. Steinman R M, Cohn Z A. 1974; The metabolism and physiology of the mononuclear phagocytes. Zweifach B W, Grant L, McCluskey R T. The inflammatory process 1 Academic Press; New York:449–510
    [Google Scholar]
  24. Stossel T P. 1974; Phagocytosis (Second of three parts). New England Journal of Medicine 290:774–780
    [Google Scholar]
  25. Styrt B, Klempner M S. 1982; Internal pH of human neutrophil lysosomes. FEBS Letters 149:113–116
    [Google Scholar]
  26. Voetman A A, Weening R S, Hamers M N, Meerhof L J, Bota A A M, Roos D. 1981; Phagocytosing human neutrophils inactivate their own granular enzymes. Journal of Clinical Investigation 67:1541–1549
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-19-1-115
Loading
/content/journal/jmm/10.1099/00222615-19-1-115
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error