1887

Abstract

Summary

Phagocytosis by human leukocytes, phagosomal H and degradation of seven species of bacteria were studied by a flow cytometric method. The percentage of phagocytosing leukocytes was similar for all bacterial strains examined, but and were more slowly phagocytosed than other bacteria. The phagosomal H surrounding the different bacterial species 15 min after the start of phagocytosis were: 4.4; 4.9; 5.1; 5.2; 5.3; 5.4; and 5.7. For longer incubation periods, the phagosomal H remained nearly constant. and were the most readily degraded of the species tested. The proteins of all bacteria were degraded more rapidly than their DNA as determined by measurements of the loss of fluorescein-isothiocyanate-fluorescence and ethidium bromide-fluorescence, respectively. The rate of degradation varied from one bacterial species to another. The degradation of proteins and DNA was maximal for bacteria residing in a phagosomal environment estimated to be between H 5.2 and 5.4.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-19-1-115
1985-02-01
2020-01-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-19-1-115
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error