The production and release of toxins A and B by Clostridium difficile during in-vitro culture was investigated. Cell-associated toxin A was detected by immunoelectrophoresis of bacterial extracts released by ultrasonication and by fluorescent antibody labelling of whole cells. Extracellular toxin A was detected by immunoelectrophoresis and by enzyme-linked immunosorbent assay; extracellular toxin B was detected by cytotoxin assay. Both toxins A and B were produced and released during the decline phase of the bacterial growth cycle. The possible significance of these results in relation to the pathogenesis of pseudomembranous colitis is discussed.
BartlettJ. G.,
TaylorN. S.1982; Antibiotic associated colitis. In
EasmonC. S. F.,
JeljaszewiczJ.
(eds) Medical microbiology vol I Academic Press; New York: p 1
BartlettJ. G.,
ChangT. W.,
GurwithM.,
GorbachS. L.,
OnderdonkA. B.1978a; Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia.. New England Journal of Medicine 298:531–534
BartlettJ. G.,
MoonN.,
ChangT. W.,
TaylorN.,
OnderdonkA. B.1978b; Role of Clostridium difficile in antibiotic associated pseudomembranous colitis.. Gastroenterology 75:778–782
GeorgeR. H.,
SymondsJ. M.,
DimockF.,
BrownJ. D.,
ArabiY.,
ShinagawaN.,
KeighleyM. R. B.,
WilliamsJ.,
BurdonD. W.1978; Identification of Clostridium difficile as a cause of pseudomembranous colitis. British Medical Journal 1:695
GeorgeR. H.,
JohnsonM.,
YoungsD.,
BurdonD. W.1980; Induction of Clostridiumdifficile toxin by antibiotics. In
NelsonJ. D.,
GrassiC.
(eds) Current chemotherapy and infectious disease American Society for Microbiology; Washington D.C: pp 955–956
JustusP. G.,
MartinJ. L.,
GoldbergD. A.,
TaylorN. S.,
BartlettJ. G.,
AlexanderR. W.,
MathiasJ. R.1982; Myoelectric effects of Clostridium difficile’. Motility-altering factors distinct from its cytotoxin and enterotoxin in rabbits.. Gastroenterology 83:836–843
KetleyJ. M.,
MitchellT. J.,
RedmondS. C.,
StephenJ.,
CandyD. C. A.,
BurdonD. W.1983; Clostridium difficile enterotoxin: amino acid composition and bacterial location. Gut 24:A999
LyerlyD. M.,
LockwoodD. E.,
RichardsonS. H.,
WilkinsT. D.1982; Biological activities of toxins A and B of Clostridum difficile. Infection and Immunity 35:1147–1150
LyerlyD. M.,
SullivanN. M.,
WilkinsT. D.1983; Enzyme-linked immunosorbent assay for Clostridium difficile toxin A.. Journal of Clinical Microbiology 17:72–78
RedmondS. C.,
KetleyJ. M.,
MitchellT. J.,
StephenJ.,
BurdonD. W.,
CandyD. C. A. Detection of Clostridium difficile enterotoxin (toxin A) by ELISA and other techniques. In Isolation and identification of organisms of medical and veterinary importance Society of Applied Bacteriology; Technical Series 21. In press
StephenJ.,
RedmondS. C.,
MitchellT. J.,
KetleyJ. M.,
CandyD. C. A.,
BurdonD. W.,
DanielR.1984; Clostridium difficile enterotoxin (toxin A): new results.. Biochemical Society Transactions. 12:194–195