Production and Release of Toxins A and B by Free

Abstract

SUMMARY

The production and release of toxins A and B by during in-vitro culture was investigated. Cell-associated toxin A was detected by immunoelectrophoresis of bacterial extracts released by ultrasonication and by fluorescent antibody labelling of whole cells. Extracellular toxin A was detected by immunoelectrophoresis and by enzyme-linked immunosorbent assay; extracellular toxin B was detected by cytotoxin assay. Both toxins A and B were produced and released during the decline phase of the bacterial growth cycle. The possible significance of these results in relation to the pathogenesis of pseudomembranous colitis is discussed.

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1984-12-01
2024-03-28
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References

  1. Bartlett J. G., Taylor N. S. 1982; Antibiotic associated colitis. In Easmon C. S. F., Jeljaszewicz J. (eds) Medical microbiology vol I Academic Press; New York: p 1
    [Google Scholar]
  2. Bartlett J. G., Chang T. W., Gurwith M., Gorbach S. L., Onderdonk A. B. 1978a; Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia.. New England Journal of Medicine 298:531–534
    [Google Scholar]
  3. Bartlett J. G., Moon N., Chang T. W., Taylor N., Onderdonk A. B. 1978b; Role of Clostridium difficile in antibiotic associated pseudomembranous colitis.. Gastroenterology 75:778–782
    [Google Scholar]
  4. Burdon D. W., Thompson H., Candy D. C. A., Kearns M., Lees D., Stephen J. 1981; Enterotoxin (s) of Clostridium difficile. Lancet 2:258–259
    [Google Scholar]
  5. George R. H., Symonds J. M., Dimock F., Brown J. D., Arabi Y., Shinagawa N., Keighley M. R. B., Williams J., Burdon D. W. 1978; Identification of Clostridium difficile as a cause of pseudomembranous colitis. British Medical Journal 1:695
    [Google Scholar]
  6. George R. H., Johnson M., Youngs D., Burdon D. W. 1980; Induction of Clostridiumdifficile toxin by antibiotics. In Nelson J. D., Grassi C. (eds) Current chemotherapy and infectious disease American Society for Microbiology; Washington D.C: pp 955–956
    [Google Scholar]
  7. Justus P. G., Martin J. L., Goldberg D. A., Taylor N. S., Bartlett J. G., Alexander R. W., Mathias J. R. 1982; Myoelectric effects of Clostridium difficile’. Motility-altering factors distinct from its cytotoxin and enterotoxin in rabbits.. Gastroenterology 83:836–843
    [Google Scholar]
  8. Ketley J. M., Mitchell T. J., Redmond S. C., Stephen J., Candy D. C. A., Burdon D. W. 1983; Clostridium difficile enterotoxin: amino acid composition and bacterial location. Gut 24:A999
    [Google Scholar]
  9. Lyerly D. M., Lockwood D. E., Richardson S. H., Wilkins T. D. 1982; Biological activities of toxins A and B of Clostridum difficile. Infection and Immunity 35:1147–1150
    [Google Scholar]
  10. Lyerly D. M., Sullivan N. M., Wilkins T. D. 1983; Enzyme-linked immunosorbent assay for Clostridium difficile toxin A.. Journal of Clinical Microbiology 17:72–78
    [Google Scholar]
  11. McDonel J. L. 1980; Clostridium perfringens types A. B, C, D, E).. Pharmacology and Therapeutics 10:617–655
    [Google Scholar]
  12. Miles A. A., Misra S. S., Irwin J. O. 1938; The estimation of the bactericidal power of the blood.. Journal of Hygiene 38:732–749
    [Google Scholar]
  13. Redmond S. C., Ketley J. M., Mitchell T. J., Stephen J., Burdon D. W., Candy D. C. A. Detection of Clostridium difficile enterotoxin (toxin A) by ELISA and other techniques. In Isolation and identification of organisms of medical and veterinary importance Society of Applied Bacteriology; Technical Series 21. In press
    [Google Scholar]
  14. Rolfe R. D., Finegold S. M. 1979; Purification and characterization of Clostridium difficile toxin.. Infection and Immunity 25:191–201
    [Google Scholar]
  15. Stephen J., Redmond S. C., Mitchell T. J., Ketley J. M., Candy D. C. A., Burdon D. W., Daniel R. 1984; Clostridium difficile enterotoxin (toxin A): new results.. Biochemical Society Transactions. 12:194–195
    [Google Scholar]
  16. Sumner H. W., Tedesco F. J. 1975; Rectal biopsy in clindamycin-associated colitis: Analysis of 23 cases.. Archives of Pathology 99:237–241
    [Google Scholar]
  17. Taylor N. S., Thorne G. M., Bartlett J. G. 1980; Separation of an enterotoxin from the cytotoxin of Clostridium difficile. Clinical Research 28:285A
    [Google Scholar]
  18. Taylor N. S., Thorne G. M., Bartlett J. G. 1981; Comparison of two toxins produced by Clostridium difficile. Infection and Immunity 34:1036–1043
    [Google Scholar]
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