1887

Abstract

Summary

The production and release of toxins A and B by during in-vitro culture was investigated. Cell-associated toxin A was detected by immunoelectrophoresis of bacterial extracts released by ultrasonication and by fluorescent antibody labelling of whole cells. Extracellular toxin A was detected by immunoelectrophoresis and by enzyme-linked immunosorbent assay; extracellular toxin B was detected by cytotoxin assay. Both toxins A and B were produced and released during the decline phase of the bacterial growth cycle. The possible significance of these results in relation to the pathogenesis of pseudomembranous colitis is discussed.

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/content/journal/jmm/10.1099/00222615-18-3-385
1984-12-01
2019-10-16
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-18-3-385
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