Growth of in Mouse Spleen Despite Loss of a Virulence Plasmid of Mol. WT 47 × 10 Free

Abstract

SUMMARY

A highly virulent strain of (LD50 . 10 bacteria/mouse) harboured two plasmids with mol. wt of 47 × 10 and 61 × 10. The role of these plasmids in virulence was studied in mice with derived strains cured of plasmids. It was confirmed that the plasmid of mol. wt 47 × 10 played a major function in virulence. This was shown both by the increase of the LD50 and the lower rate of multiplication in the spleen obtained with strains cured of the plasmid of mol. wt 47 × 10. The plasmid of mol. wt 61 × 10 did not play any role in virulence. This work also demonstrates that the strain cured of the plasmid of mol. wt 47 × 10 and the plasmid-free strain were able to multiply in the spleens of infected mice during a 7-day period. This suggests that virulence factors not associated with plasmids are also responsible for the bacterial growth in tissues .

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1984-12-01
2024-03-28
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References

  1. Ben-Gurion R., Shafferman A. 1981; Essential virulence determinants of different Yersinia species are carried on a common plasmid.. Plasmid 5:183–187
    [Google Scholar]
  2. Berche P. A., Carter P. B. 1982; Calcium requirement and virulence of Yersinia enterocolitica. Journal of Medical Microbiology 15:277–284
    [Google Scholar]
  3. Bölin I., Norlander L., Wolf-Watz H. 1982; Temperature-inducible outer membrane protein of Yersinia pseudotuberculosis and Yersinia enterocolitica is associated with the virulence plasmid.. Infection and Immunity 37:506–512
    [Google Scholar]
  4. Brubaker R. R. 1972; The genus Yersinia: biochemistry and genetics of virulence.. Current Topics in Microbiology and Immunology 57:111–158
    [Google Scholar]
  5. Cuzin F., Jacob F. 1967; Mutations de l’episome F d’Escherichia coli K.12. 2. Mutants a replication thermosensible.. Annales de l’lnstitut Pasteur 112:397–418
    [Google Scholar]
  6. Ferber D. M., Brubaker R. R. 1981; Plasmids in Yersiniapestis. Infection and Immunity 31:839–841
    [Google Scholar]
  7. Gemski P., Lazere J. R., Casey T. 1980a; Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infection and Immunity 27:682–685
    [Google Scholar]
  8. Gemski P., Lazere J. R., Casey T., WohlhieterJ A. 1980b; Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis. Infection and Immunity 28:1044–1047
    [Google Scholar]
  9. Maki M., Yesikari T., Rantala I., Sundqvist C., Gronroos P. 1983; Pathogenicity of 42–44 Mdal plasmid positive and negative. Yersinia pseudotuberculosis 1 and Yersinia enterocolitica Section B 91:241–244
    [Google Scholar]
  10. Mazigh D., Chalvignac M. A., Alonso J. M., Mollaret H. H. 1983; Determinisme genetique de la virulence de Yersinia enterocolitica et de l’immunogénicité croisee contre Yersinia pestis. In Les bacilles à Gram négatif d’intérêt medical et en Santé Publique: Taxonomie-Identifica tion-Applications, les Colloques de 1’INSERM.. INSERM 114:517–535
    [Google Scholar]
  11. Mollaret H. H. 1965; Les formes cliniques de l’infection humaine a bacille de Malassez et Vignal.. Pathologie et Biologie 13:554–566
    [Google Scholar]
  12. Portnoy D. A., Falkow S. 1981; Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis. Journal of Bacteriology 148:877–883
    [Google Scholar]
  13. Portnoy D. A., Moseley S. L., Falkow S. 1981; Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis.. Infection and Immunity 31:775–782
    [Google Scholar]
  14. Simonet M., Berche P., Mazigh D. 1983; Une septicemie due a une souche hautement virulente de Yersinia pseudotuberculosis. Medecine et Maladies Infectieuses 13:767–771
    [Google Scholar]
  15. Vesikari T., Nurmi T., Maki M., Skurnik M., Sundqvist C., Granfors K., Gronroos P. 1981; Plasmids in Yersinia enterocolitica serotypes 0:3 and 0:9: correlation with epithelial cell adherence in vitro.. Infection and Immunity 33:870–876
    [Google Scholar]
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