1887

Abstract

SUMMARY

The adhesion of leptospires ( serovar. copenhageni L45) to mouse L-cells was studied by microscopic observations. Within 3 h of infection of monolayers many leptospires adhered to 95-100% of the cells, and intracellular leptospires were demonstrated by electron microscopy. No specific site of attachment on the cells or the leptospires was observed. Avirulent or dead leptospires adhered poorly but attachment of the saprophytic leptospire serovar. occurred on cell and glass surfaces. After adhesion, microvilli on the cell surfaces disappeared within 6 h of infection and cell damage was observed after 12 h. The adhesion was greatly enhanced by the presence of specific antiserum at a subagglu-tinating concentration. No direct penetration by leptospires of the host cells was observed with transmission and scanning electron microscopy. It appears that (1) adhesion of leptospires to L-cells precedes cell damage, and (2) leptospires may enter cells either through damaged membranes, or by a phagocytosis-like mechanism.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-18-1-73
1984-08-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/18/1/medmicro-18-1-73.html?itemId=/content/journal/jmm/10.1099/00222615-18-1-73&mimeType=html&fmt=ahah

References

  1. Adler B., Faine S. 1978; Serological and protective-antibody responses of rabbits to leptospiral antigens. Journal of Medical Microbiology 11:401–409
    [Google Scholar]
  2. Alston M. J., Broom J. C. 1958 Leptospirosis in man and animals Livingstone; Edinburgh:
    [Google Scholar]
  3. Arean M. V., Sarasin G., Green J. H. 1964; The pathogenesis of leptospirosis: Toxin production by leptospira icterohaemorrhagiae. American Journal of Veterinary Research 25:836–843
    [Google Scholar]
  4. Babudieri B. 1958; Animal reservoirs of leptospires. Annals of the New York Academy of Science 70:393–413
    [Google Scholar]
  5. Byrne G. I., Moulder J. W. 1978; Parasite-specified phagocytosis of Chlamydia psittaci and Chlamydia trachomatis by L and Hela cells. Infection and Immunity 19:598–606
    [Google Scholar]
  6. Chaperon K. M., Davis R. B., Miller N. G. 1979; Effect of a plasma cytotoxic factor in leptospirosis on platelets in vivo. Thrombosis Research 14:975–980
    [Google Scholar]
  7. Cox P. J., Twigg G. I. 1974 Leptospiral motility. Nature (London) 250:260–261
    [Google Scholar]
  8. DeBrito T., Freymuller E., Hoshino S., Penna D. O. 1966; Pathology of the kidney and liver in the experimental leptospirosis of the guinea pig. A light and electron microscopy study. Virchows Archiv Pathologische Anatomie 341:64–78
    [Google Scholar]
  9. DeBrito T., Bohm G. M., Yasuda P. H. 1979; Vascular damage in acute experimental leptospirosis of the guinea pig. Journal of Pathology 128:177–182
    [Google Scholar]
  10. Faine S. 1962; Factors affecting the development of the carrier state in leptospirosis. Journal of Hygiene 60:427–434
    [Google Scholar]
  11. Faine S. 1963; Antibody in the renal tubules and urine of mice. Australian Journal of Experimental Biology and Medical Science 41:81–91
    [Google Scholar]
  12. Faine S. 1965; Silver staining of spirochaetes in single tissue sections. Journal of Clinical Pathology 18:381–382
    [Google Scholar]
  13. Faine S., Hudson R. E., Frank R. 1975; Pathogenic mechanisms in leptospirosis. Cytopathic effects. In Proceedings of the National Symposium on Leptospirosis, Leptospira and other Spirochaeta-Bucharest September 25-27, 1975 Editura Medicara, Bucharest; Romania:145–149
    [Google Scholar]
  14. Faine S., van der Hoeden J. 1964; Virulence-linked colonial and morphological variation in Leptospira. Journal of Bacteriology 88:1493–1496
    [Google Scholar]
  15. Fedoroff S., Webb S. J. 1962; Natural cytotoxic antibodies in human blood sera which react with mammalian cells and bacteria. Nature (London) 193:80–81
    [Google Scholar]
  16. Finn M. A., Jenkin H. M. 1973; Cytopathic effects of Leptospira serotypes patoc and canicola in three kidney cell culture systems. American Journal of Veterinary Research 34:669–672
    [Google Scholar]
  17. Fitzgerald T. J., Johnson R. C., Miller J. N., Sykes J. A. 1977; Characterization of the attachment of Treponema pallidum (Nichols strain) to cultured mammalian cells and the potential relationship of attachment to pathogenicity. Infection and Immunity 18:467–478
    [Google Scholar]
  18. Hale T. L., Bonventre P. F. 1979; Shigella infection of Henle intestinal epithelial cells: role of the bacterium. Infection and Immunity 24:879–886
    [Google Scholar]
  19. Harrington D. D., Sleight S. D. 1966; Leptospira pomona in tissue culture: Preliminary study. American Journal of Veterinary Research 27:249–256
    [Google Scholar]
  20. Holt L. B. 1972; The pathology and immunology of Bordetella pertussis infection. Journal of Medical Microbiology 5:407–424
    [Google Scholar]
  21. Linscott W. D., Boak R. A. 1961; Immune adherence with Leptospira antigens. I. Studies on the immune-adherence phenomenon. Journal of Immunology 86:471–479
    [Google Scholar]
  22. Marshall R. B. 1974; Ultrastructural changes in renal tubules of sheep following experimental infections with Leptospira interrogans serotype pomona. Journal of Medical Microbiology 7:505–508
    [Google Scholar]
  23. Miller N. G., Froehling R. C., White R. J. 1970; Activity of leptospires and their products on L cell monolayers. American Journal of Veterinary Research 31:371–377
    [Google Scholar]
  24. Miller N. G., Wilson R. B. 1962; In vivo and in vitro observations of Leptospira pomona by electron microscopy. Journal of Bacteriology 84:569–576
    [Google Scholar]
  25. Miller N. G., Wilson R. B. 1967; Electron microscopic study of the relationship of Leptospira pomona to the renal tubules of the hamster during acute and chronic leptospirosis. American Journal of Veterinary Research 28:225–235
    [Google Scholar]
  26. Rose G. W., Eveland W. C., Ellinghausen H. C. 1966; Mechanisms of tissue cell penetration by Leptospira pomona: Active penetration studies in vitro. American Journal of Veterinary Research 27:1461–1471
    [Google Scholar]
  27. Rowley D., Jenkin C. R. 1962; Antigenic cross-reaction between host and parasite as a possible cause of pathogenicity. Nature (London) 193:151–154
    [Google Scholar]
  28. Rothstein N. 1957; Studies of the immunochemistry of leptospires. II. Heterogenetic relationship between leptospires and other microorganisms. Journal of Immunology 79:276–280
    [Google Scholar]
  29. Shinitzky M., Inbar M. 1976; Microviscosity parameters and protein mobility in biological membranes. Biochemica et Biophysica Acta 433:133–149
    [Google Scholar]
  30. Smith H. 1977; Microbial surfaces in relation to pathogenicity. Bacteriological Reviews 41:475–500
    [Google Scholar]
  31. Sykes J. A., Moore E. B. 1960; A simple tissue culture chamber. Texas Reports on Biology and Medicine 18:288–297
    [Google Scholar]
  32. van Oss C. J. 1978; Phagocytosis as a surface phenomenon. Annual Review of Microbiology 32:19–39
    [Google Scholar]
  33. Yam P. A., Miller N. G., White R. J. 1970; A leptospiral factor producing a cytopathic effect on L cells. Journal of Infectious Diseases 122:310–317
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-18-1-73
Loading
/content/journal/jmm/10.1099/00222615-18-1-73
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error