A total of 57 strains of Acinetobacter calcoaceticus was fingerprinted by SDS-PAGE of cellular protein. All strains were also examined by conventional, API and N/F-Tek methods, and antibiotic sensitivity patterns were determined. In general, using the API 20E and N/F-Tek methods, it was possible to assign isolates of A. calcoaceticus to the two accepted “biotypes” or “varieties”, A.c. anitratus and A.c. Iwoffi, but these methods did not offer useful subdivision of the biotypes. Gel electrophoresis permitted subdivision of the strains into clusters in a manner which suggests that the technique may be valuable in typing strains isolated during outbreaks of infection in hospital.
Al KhojaM. S.,
DarrellJ. R.1979; The skin as a source of Acinetobacter and Moraxella species occurring in blood cultures. Journal of Clinical Pathology 32:497–499
BrisouJ.,
PrévotA. R.1954; Etudies de systématique bactérienne. X. Révision des espéces réunies dans le genre Achromobacter. Annales de L’Institut Pasteur 86:722–728
BuxtonA. E.,
AndersonR. L.,
WerdegarD.,
AtlasE.1978; Nosocomial respiratory tract infection and colonization with Acinetobacter calcoaceticus. American Journal of Medicine 65:507–513
FrenchG. L.,
CasewellM. W.,
RoncoroniA. J.,
KnightS.,
PhillipsI.1980; A hospital outbreak of antibiotic-resistant Acinetobacter anitratus: epidemiology and control. Journal of Hospital Infection 1:125–131
HoppeM.,
PotelJ.,
MalottkeR.1983; Acinetobacter calcoaceticus isolation from blood and venous catheters: contamination, asymptomatic bacteremia or septicemia?. Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene I., Abteilung Originate, A 253:452
International Committee on Nomenclature of Bacteria. Sub committee on the taxonomy of Moraxella and allied bacteria1971International Journal of Systematic Bacteriology 21:213–214
JackmanP. J. H.1982; Classification of Corynebacterium species from axillary skin by numerical analysis of electrophoretic protein patterns. Journal of Medical Microbiology 15:485–492
JackmanP. J. H.1983; Characterisation of microorganisms by electrophoretic protein patterns. In
GutteridgeC. S.
(ed) New methods for the detection and characterisation of microorganisms. John Wiley and Sons; Chichester: (in press)
JackmanP. J. H.,
FelthamR. K. A.,
SneathP. H. A.1983; A program in BASIC for numerical taxonomy of microorganisms based on electrophoretic protein patterns. Microbios Letters 23:87–98
MartinR.,
RileyP. S.,
HollisD. G.,
WeaverR. E.,
KrichevskyM. I.1981; Characterization of some groups of gram negative non-fermentative bacteria by the carbon source alkalinization technique. Journal of Clinical Microbiology 14:39–47
MorganM. E. I.,
HartC. A.1982; Acinetobacter meningitis: acquired infection in a neonatal intensive care unit. Archives of Diseases of Childhood 57:557–559
RetailliauH. F.,
HightowerA. W.,
DixonR. E.,
AllenJ. R.1979; Acinetobacter calcoaceticus: a nosocomial pathogen with an unusual seasonal pattern. Journal of Infectious Diseases 139:371–375
SneathP. H. A.,
JohnsonR.1972; The influence on numerical taxonomic similarities of errors in microbiological tests. Journal of General Microbiology 72:377–392
TorregrosaM. V.,
OrtizA.1961; Severe infections in children due to rare gram-negative bacilli (Mima polymorpha and Bacillus anitratum). Journal of Pediatrics 59:35–41