1887

Abstract

SUMMARY

Twenty eight strains of , isolated from an outbreak of antibiotic-associated colitis and diarrhoea in an orthopaedic ward and from sporadic cases throughout Sweden, were sent to Edinburgh for immunochemical fingerprinting without information about their origin. EDTA extracts of the organisms were examined by crossed immunoelectrophoresis (CIE), polyacrylamide gel electrophoresis (PAGE) and electroblot transfer. Two patterns were revealed by CIE: group A (18 strains) and group B (10 strains). PAGE and electroblot transfer revealed one major group of 10 strains (group 1), six small groups of two or three strains and six strains which were unlike any other strain. The CIE group B and PAGE-electroblot group 1 were identical. Nine of the 10 strains in this gioup were from patients in the outbreak. These findings indicate that a single strain spread in the orthopaedic ward as a nosocomial infection and that this strain differed from most other strains investigated. The PAGE-electroblot technique should, therefore, greatly aid investigations into the epidemiology of .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-17-3-317
1984-06-01
2022-12-02
Loading full text...

Full text loading...

/deliver/fulltext/jmm/17/3/medmicro-17-3-317.html?itemId=/content/journal/jmm/10.1099/00222615-17-3-317&mimeType=html&fmt=ahah

References

  1. British Medical Journal (Leading Article) 1981; Antibiotic-associated colitis—the continuing saga. British Medical Journal 282:1913–1914
    [Google Scholar]
  2. Aronsson B., Mollby R., Nord C. E. 1981; Occurrence of toxin-producing Clostridium difficile in antibiotic-associated diarrhoea in Sweden. Medical Microbiology and Immunology 170:27–35
    [Google Scholar]
  3. Bartlett J. G. 1979; Antibiotic-associated pseudomembranous colitis. Review of Infectious Diseases 1:530–539
    [Google Scholar]
  4. Borriello S. P., Honour P., Turner T., Barclay F. 1983; Household pets as a potential reservoir for Clostridium difficile infection. Journal of Clinical Pathology 36:84–87
    [Google Scholar]
  5. Brettle R. P., Poxton I. R., Murdoch J. McC., Brown R., Byrne M. D., Collee J. G. 1982; Clostridium difficile in association with sporadic diarrhoea. British Medical Journal 284:230–233
    [Google Scholar]
  6. Fekety R., Kim K-H., Brown D., Batts D. H., Cudmore M., Silva J. 1981; Epidemiology of antibiotic-associated colitis. Isolation of Clostridium difficile from the hospital environment. American Journal of Medicine 70:906–908
    [Google Scholar]
  7. George W. L., Sutter V. L., Finegold S. M. 1977; Antimicrobial agent-induced diarrhea—a bacterial disease. Journal of Infectious Diseases 136:822–828
    [Google Scholar]
  8. Greenfield C., Burroughs A., Szawathowski M., Bass N., Noone P., Pounder R. 1981; Is pseudomembranous colitis infectious?. Lancet 1:371–372
    [Google Scholar]
  9. Hafiz S., Oakley C. L. 1976; Clostridium difficile: Isolation and characteristics. Journal of Medical Microbiology 9:129–136
    [Google Scholar]
  10. Holbrook W. P., Duerden B. I., Deacon A. G. 1977; The classification of Bacteroides melanino-genicus and related species. Journal of Applied Bacteriology 42:259–273
    [Google Scholar]
  11. Howard J. M., Sullivan S. N., Troster M. 1980; Spontaneous pseudomembranous colitis. British Medical Journal 281:356
    [Google Scholar]
  12. Kim K. H., Fekety R., Batts D. H., Brown D., Cudmore M., Silva J., Waters D. 1981; Isolation of Clostridium difficile from the environment and contacts of patients with antibiotic-associated colitis. Journal of Infectious Diseases 143:42–50
    [Google Scholar]
  13. Kim K. H., DuPont H. L., Pickering L. K. 1983; Outbreaks of diarrhea with Clostridium difficile and its toxin in day-care centres: Evidence of person to person spread. Journal of Paediatrics 102:376–382
    [Google Scholar]
  14. Laemmli U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature, London: 227:680–685
    [Google Scholar]
  15. Larson H. E., Price A. B., Honour P., Borriello S. P. 1978; Clostridium difficile and the aetiology of pseudomembranous colitis. Lancet 1:1063–1066
    [Google Scholar]
  16. Larson H. E., Price A. B., Borriello S. P. 1980; Epidemiology of experimental enterocitis due to Clostridium difficile. Journal of Infectious Diseases 142:408–413
    [Google Scholar]
  17. Larson H. E., Barclay F. E., Honour P., Hill I. D. 1982; Epidemiology of Clostridium difficile in infants. Journal of Infectious Diseases 146:727–733
    [Google Scholar]
  18. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  19. Mollby R., Nord C. E., Aronsson B. 1980; Diagnosis of Clostridium difficile- associated enterocolitis in Sweden. Laboratory and epidemiological aspects. Scandinavian Journal of Infectious Diseases Suppl 2230–36
    [Google Scholar]
  20. Mulligan M. E., George W. L., Rolfe R. D., Finegold S. M. 1980; Epidemiological aspects of Clostridium difficile induced diarrhea and colitis. American Journal of Clinical Nutrition 33:2533–2538
    [Google Scholar]
  21. Orchard J. L., Fekety R., Smith J. R. 1983; Antibiotic-associated colitis due to Clostridium difficile in a Kodiak bear. American Journal of Veterinary Research 44:1547–1548
    [Google Scholar]
  22. Poxton I. R. 1982; Detection and isolation of Clostridium difficile. European Journal of Chemotherapy and Antibiotics 2:123–128
    [Google Scholar]
  23. Poxton I. R., Brown R. 1979; Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of cell-surface proteins as an aid to the identification of the Bacteroides fragilis group. Journal of General Microbiology 112:211–217
    [Google Scholar]
  24. Poxton I. R., Byrne M. D. 1981; Immunological analysis of the EDTA-soluble antigens of Clostridium difficile and related species. Journal of General Microbiology 122:4–46
    [Google Scholar]
  25. Rogers T. R., Petrou M., Lucas C., Chung J. T. N., Barrett A. J., Borriello S. P., Honour P. 1981; Spread of Clostridium difficile among patients receiving non-absorbable antibiotics for gut decontamination. British Medical Journal 283:408–409
    [Google Scholar]
  26. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  27. Weeke B. 1973; Crossed immunoelectrophoresis. Scandinavian Journal of Immunology 2 Suppl 147–56
    [Google Scholar]
  28. Wüst J., Sullivan N. M., Hardegger U., Wilkins T. D. 1982; Investigation of an outbreak of antibiotic-associated colitis by various typing methods. Journal of Clinical Microbiology 16:1096–1101
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-17-3-317
Loading
/content/journal/jmm/10.1099/00222615-17-3-317
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error