1887

Abstract

SUMMARY

Antigens prepared from several strains of each of 10 species were used in an indirect enzyme-linked immunosorbent assay with antisera raised against whole cells of a representative strain from each of the 10 species killed by ultra-violet irradiation. With the exception of , the antisera gave similar results with antigens prepared from all strains of the homologous species. Antigens prepared from 13 other clostridial species were then investigated in an ELISA system with the 10 representative antisera. The results showed many cross-reactions, particularly in the group, the group and the group.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-17-2-171
1984-04-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/17/2/medmicro-17-2-171.html?itemId=/content/journal/jmm/10.1099/00222615-17-2-171&mimeType=html&fmt=ahah

References

  1. Batty I., Walker P. D. 1965; Colonial morphology and fluorescent labelled antibody staining in the identification of species of the genus Clostridium. Journal of Applied Bacteriology 28:112–118
    [Google Scholar]
  2. Cruickshank R., Duguid J. P., Marmion B. P., Swain R. H. A. Eds. 1975 Clostridium I. In: Medical Microbiology, Vol 2: 12th ed Churchill Livingstone; Edinburgh: pp 471–488
  3. Hatheway C. L., McCroskey L. M. 1981; Laboratory investigation of human and animal botulism. In Biomedical aspects of botulism edited by Lewis G. E. Academic Press; New York: pp 165–180
    [Google Scholar]
  4. Holbrook W. P., Duerden B. I., Deacon A. G. 1977; The classification of Bacteroides melanino-genicus and related species. Journal of Applied Bacteriology 42:259–273
    [Google Scholar]
  5. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe laboratory manual 4th ed Virginia Polytechnic and State University; Blacksburg, Virginia:
    [Google Scholar]
  6. Hubert J., Ionesco H., Sebald M. 1981; Detection de Clostridium difficile par isolement sur milieu minimal selectif et par immunofluorescence. Annals of Microbiology (Institute Pasteur) 132A:149–157
    [Google Scholar]
  7. Lynt R. K., Solomon H. N., Kautter D. A. 1971; Immunofluorescence among strains of Clostridium botulinum and other clostridia by direct and indirect methods. Journal of Food Science 36:594–599
    [Google Scholar]
  8. Mandia J. W. 1955; The position of Clostridium tetani within the serological schema for the proteolytic clostridia. Journal of Infectious Diseases 97:66–72
    [Google Scholar]
  9. Poxton I. R. 1979; Serological identification of Bacteroides species by an enzyme-linked immunosorbent assay. Journal of Clinical Pathology 32:294–298
    [Google Scholar]
  10. Poxton I. R., Brown R., Collee J. G. 1982; Detection of species-specific and cross-reactive cell-surface antigens of Bacteroides species by an indirect enzyme-linked immunosorbent assay. Journal of Medical Microbiology 15:223–231
    [Google Scholar]
  11. Poxton I. R., Byrne M. D. 1981a; Immunological analysis of the EDTA-soluble antigens of Clostridium difficile and related species. Journal of General Microbiology 122:41–46
    [Google Scholar]
  12. Poxton I. R., Byrne M. D. 1981b; Detection of Clostridium difficile toxin by counterimmunoelectro-phoresis: a note of caution. Journal of Clinical Microbiology 14:349
    [Google Scholar]
  13. Poxton I. R., Cartmill T. D. I. 1982; Immunochemistry of the cell-surface carbohydrate antigens of Clostridium difficile. Journal of General Microbiology 128:1365–1370
    [Google Scholar]
  14. Smith L. D. S. 1977 Botulism, the organism, its toxin, the disease Thomas; Springfield, Illinois: pp 15–33
    [Google Scholar]
  15. Walker P. D., Batty I. 1964; Fluorescent studies in the genus Clostridium. II. A rapid method for differentiating Clostridium botulinum types, A, B and, F, types C and, D, and type E. Journal of Applied Bacteriology 27:140–142
    [Google Scholar]
  16. Willis A. T. 1969 Clostridia of wound infection Butterworths; London: pp 1–40
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-17-2-171
Loading
/content/journal/jmm/10.1099/00222615-17-2-171
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error