Skip to content
1887

Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 17, Issue 1

Chemotaxis of human polymorphonuclear leukocytes toward No Access

Abstract

SUMMARY

Chemoattractive properties of were studied by measuring leukocyte migration in agarose gel. Human serum albumin (0.5%) was present in the gel and normal human serum was excluded from all components of the assay. Viable cell populations and lysates of colonial types F62T1, F62T2 and F62T3 induced migration of polymorphonuclear leukocytes. Chemotactic activity of the lysate was not altered by heating at 100°C for 10 min and was retained in the 12 100 supernatant fraction of the heated lysate. Fractionation of the supernate by Sephadex G-100 chromatography showed that the chemotactic activity was associated primarily with an absorbance peak at 280 nm of relatively low mol. wt. The chemotactic activity of this fraction was lost after dialysis and the peak was no longer present in the Sephadex G-100 elution profile of the dialysed supernate. The gonococcal leukotaxins were sensitive to digestion by trypsin, pronase and amyloglucosidase, but insensitive to treatment with RNAase, DNAase or lipase at H 5.7-7.1.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1984
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-17-1-45
1984-02-01
2025-01-20
Loading full text...

Full text loading...

References

  1. Baum J., Mowat A. G., Kirk J. A. 1971; A simplified method for the measurement of chemotaxis of polymorphonuclear leukocytes from human blood. Journal of Laboratory and Clinical Medicine 19:501–509
     [Google Scholar]
  2. Bier M. 1955; Lipases. In Colowick S. P., Kaplan N. O. (eds) Methods in enzymology vol I: Academic Press; New York: pp 627–642
     [Google Scholar]
  3. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350
     [Google Scholar]
  4. Gerhardt P., Heden C. G. 1960; Concentrated culture of gonococci in clear liquid medium. Proceedings of the Society for Biology and Medicine 105:49–51
     [Google Scholar]
  5. Hartree E. F. 1972; Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analytical Biochemistry 48:422–427
     [Google Scholar]
  6. Hill H. R., Hogan N. A., Mitchell T. G., Quie P. G. 1975; Evaluation of a cytocentrifuge method for measuring neutrophil granulocyte chemotaxis. Journal of Laboratory and Clinical Medicine 86:703
     [Google Scholar]
  7. James A. N., Williams R. P. 1978; Chemotactic effect of Neisseria gonorrhoeae on polymorphonuclear leukocytes. In Brooks G. F. (eds) Immunobiology of Neisseria gonorrhoeae American Society for Microbiology; Washington, DC,: pp 236–238
     [Google Scholar]
  8. Keller H. U., Sorkin E. 1967; Studies on chemotaxis. V. On the chemotactic effect of bacteria. International Archives of Allergy and Applied Immunology 31:505–517
     [Google Scholar]
  9. Kellogg D. S., Peacock W. L., Deacon W. E., Brown L., Pirkle C. 1963; Neisseria gonorrhoeae. I: Virulence genetically linked to clonal variation. Journal of Bacteriology 85:1274–1279
     [Google Scholar]
  10. Klipstein F. A., Engert R. F. 1976; Purification and properties of Klebsiella pneumoniae heat stable enterotoxin. Infection and Immunity 13:373–381
     [Google Scholar]
  11. Kunitz M. 1946; RNAase A. In Biochemica Information–1974 Boehringer Mannheim, Mannheim; West Germany: pp 156–157
     [Google Scholar]
  12. Kunitz M. 1950; DNAase I. In Biochemica Information–1974 Boehringer Mannheim, Mannheim; West Germany: p 80
     [Google Scholar]
  13. Nelson R. D., Quie P. G., Simmons R. L. 1975; Chemotaxis under agarose: A new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. Journal of Immunology 115:1650–1656
     [Google Scholar]
  14. Pazur J. H., Kleppe K. 1962; The hydrolysis of a-D-glucosides by amyloglucosidase from Aspergillus niger. Journal of Biological Chemistry 237:1002
     [Google Scholar]
  15. Rick W. 1963; Trypsin. In Bergmeyer H. U. (ed) Methods of enzymatic analysis Academic Press; New York: pp 807–818
     [Google Scholar]
  16. Schiffmann E., Showed H. V., Corcoran B. A., Ward P. A., Smith E., Becker E. L. 1975; The isolation and partial characterization of neutrophil chemotactic factors from Escherichia coli. Journal of Immunology 114:1831–1837
     [Google Scholar]
  17. Ward M. E., Watt P. J. 1972; Adherence of Neisseria gonorrhoeae to urethral mucosa cells: An electron microcopic study of human gonorrhea. Journal of Infectious Diseases 126:601–605
     [Google Scholar]
  18. Ward M. E., Watt P. J., Robertson J. N. 1974; The human fallopian tube: A laboratory model for gonococcal infection. Journal of Infectious Diseases 129:650–659
     [Google Scholar]
  19. Ward P. A., Lepow I. H., Newman L. J. 1968; Bacterial factors chemotactic for polymorphonuclear leukocytes. American Journal of Pathology 52:725–736
     [Google Scholar]
  20. Watt P. J., Medlen A. R. 1978; Generation of chemotaxins by gonococci. In Brooks G. F. (eds) Immunobiology of Neisseria gonorrhoeae American Society for Microbiology; Washington, DC: pp 239–241
     [Google Scholar]
  21. Wilkinson P. C. 1974; Outline of a method for measuring chemotaxis (Appendix). In Chemotaxis and inflammation Churchill Livingstone; Edinburgh: pp 168–172
     [Google Scholar]
/content/journal/jmm/10.1099/00222615-17-1-45
Loading
Chemotaxis of human polymorphonuclear leukocytes toward Neisseria gonorrhoeae
J. Med. Microbiol. 17, 45 (1984); https://doi.org/10.1099/00222615-17-1-45
/content/journal/jmm/10.1099/00222615-17-1-45
/content/journal/jmm/10.1099/00222615-17-1-45
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error