RT Journal Article SR Electronic(1) A1 Magee, J. T. A1 Hindmarch, J. M. A1 Meechan, D. F.YR 1983 T1 Identification of staphylococci by pyrolysis gas-liquid chromatography JF Journal of Medical Microbiology, VO 16 IS 4 SP 483 OP 495 DO https://doi.org/10.1099/00222615-16-4-483 PB Microbiology Society, SN 1473-5644, AB SUMMARY The identification of bacteria by pyrolysis gas-liquid chromatography (PGLC) was evaluated with the genus Staphylococcus. A matrix containing the characteristics of seven species of Staphylococcus was constructed from the results obtained with 42 named strains identified by biochemical methods. An identification program based upon this database was evaluated in tests with 100 isolates of catalase-positive oxidase-negative gram-positive cocci identified by biochemical and PGLC systems. Agreement between the two systems was 90%. Pure cultures of unknown isolates were identified within 30 min by PGLC using a microcomputer data acquisition and analysis program. A taxonomic analysis of the data is presented, and methods for quality control of PGLC, prolongation of column life, and data analysis are described and discussed., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-16-4-483