1887

Abstract

SUMMARY

In tests for IgG antibodies against Coxsackie B viruses in man, the enzyme-linked immunosorbent assay (ELISA) was essentially group-specific and, unlike the type-specific neutralisation test, usually failed to detect rises in antibody titre in paired, acute and convalescent, sera. However, in rabbits immunised against Coxsackie B viruses, ELISA demonstrated both group- and type-specific antibody responses. The lack of type-specificity of ELISA in man is probably because repeated infection with enteroviruses—echoviruses and Coxsackie A as well as Coxsackie B—results in masking of the type-specific antibody response by group-specific antibody.

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1983-11-01
2023-02-04
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References

  1. Bell E. J., Grist N. R. 1968; Coxsackie virus infections in patients with acute cardiac disease and chest pain. Scottish Medical Journal 13:47–51
    [Google Scholar]
  2. Booth J. C., Hannington G., Aziz T. A. G., Stern H. 1979; Comparison of enzyme-linked immunosorbent assay (ELISA) technique and complement-fixation test for estimation of cytomegalovirus IgG antibody. Journal of Clinical Pathology 32:122–127
    [Google Scholar]
  3. Breindl M. 1971; The structure of heated poliovirus particles. Journal of General Virology 11:147–156
    [Google Scholar]
  4. Brown F., Wild T. F., Rowe L. W., Underwood B. O., Harris T. J. R. 1976; Comparison of swine vesicular disease virus and Coxsackie B5 virus by serological and RNA hybridization methods. Journal of General Virology 31:231–237
    [Google Scholar]
  5. Chaudhary R. K., Westwood J. C. N. 1970; Detection of cross-reactions by immunofluorescence within the picornavirus group. Canadian Journal of Microbiology 16:601–604
    [Google Scholar]
  6. Cramblet H.G., Moffet H. L., Black J. P., Shulenberger H., Smith A., Colonna C. T. 1964; Coxsackie virus infections in clinical laboratory studies. Journal of Pediatrics 64:406–414
    [Google Scholar]
  7. Doerr H. W., Schweiss H. D., Henninger K., Sochanik H. 1977; Simple mathematical deductions in the seroepidemiology of viral infections. Ⅱ (Para) myxoviruses (measles, mumps, influenza B), rubella, enteroviruses (polio, Coxsackie B), adenoviruses, and Mycoplasma pneumoniae. Zentralblattfur Bakteriologie, Parasitenkunde, Infektionskrank-heiten und Hygiene. I. Abt. Orig A238:165
    [Google Scholar]
  8. El-Hagrassy M. M. O., Banatvala J. E., Coltart D. J. 1980; Coxsackie B virus specific IgM responses in patients with cardiac and other diseases. Lancet 2:1160–1162
    [Google Scholar]
  9. Engvall E., Perlmann P. 1971; Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8:871–874
    [Google Scholar]
  10. Griffiths P. D., Hannington G., Booth J. C. 1980; Coxsackie B virus infections and myocardial infarction. Results from a prospective, epidemiologically controlled study. Lancet 1:1387–1389
    [Google Scholar]
  11. Grist N. R., Bell E. J., Assaad F. 1978; Enteroviruses in human disease. Progress in Medical Virology 24:114–157
    [Google Scholar]
  12. Halonen P., Rosen L., Huebner R. J. 1959; Homologous and heterologous complement fixing antibody in persons infected with ECHO, Coxsackie and Poliomyelitis viruses. Proceedings of the Society for Experimental Biology and Medicine 101:236–241
    [Google Scholar]
  13. Herrmann J. E., Hendry R. M., Collins M. F. 1979; Factors involved in enzyme-linked immunoassay of viruses and evaluation of the method for identification of enteroviruses. Journal of Clinical Microbiology 10:210–217
    [Google Scholar]
  14. Katze M. G., Crowell R. L. 1980a; Indirect enzyme-linked immunosorbent assay (ELISA) for the detection of Coxsackievirus group B antibodies. Journal of General Virology 48:225–229
    [Google Scholar]
  15. Katze M. G., Crowell R. L. 1980b; Immunological studies of the group B Coxsackieviruses by the sandwich enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation. Journal of General Virology 50:357–367
    [Google Scholar]
  16. Lonberg-Holm K., Yin F. H. 1973; Antigenic determinants of infective and inactivated human rhinovirus type 2. Journal of Virology 12:114–123
    [Google Scholar]
  17. Maizel J. V., Phillips B. A., Summers D. E. 1967; Composition of artificially produced and naturally occurring empty capsids of poliovirus type 1. Virology 32:692–699
    [Google Scholar]
  18. Schmidt N. J., Dennis J., Frommhagen L. H., Lennette E. H. 1963; Serologic reactivity of certain antigens obtained by fractionation of Coxsackie viruses in cesium chloride density gradients. Journal of Immunology 90:654–662
    [Google Scholar]
  19. Schmidt N. J., Dennis J., Lennette E. H. 1967; Antibody responses of rhesus (Macaca mulatto) monkeys experimentally infected with Coxsackieviruses of group B and group A, type 9. Ⅱ Heterotypic antibody responses to echoviruses, polioviruses and reovirus type 1. Journal of Immunology 98:1060–1066
    [Google Scholar]
  20. Schmidt N. J., Dennis J., Lennette E. H., Ho H. H., Shinomoto T. T. 1965; Antibody responses of rhesus (Macaca mulatto) monkeys experimentally infected with Coxsackieviruses of group B and group A, type 9. I Antibody responses within the Coxsackievirus group. Journal of Immunology 95:54–69
    [Google Scholar]
  21. Van Weemen B. K., Schuurs A. H. W. M. 1971; Immunoassay using antigen-enzyme conjugates. FEBS Letters 15:232–236
    [Google Scholar]
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