Detection of enterotoxin by tissue culture and double-gel diffusion methods Free

Abstract

SUMMARY

Culture filtrates of four enterotoxin-producing strains of type A produced morphological changes in cells of 10 of 12 established mammalian cell lines tested, and particularly Vero (African green-monkey kidney) and MDCK (dog kidney) cells. Double-gel diffusion and Vero-cell tests detected enterotoxin in culture filtrates of, respectively, 29 (28%) and 56 (54%) of 103 strains of tested. The feasibility of cell-culture assays for the routine detection of enterotoxin is indicated.

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1983-05-01
2024-03-29
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