Culture filtrates of four enterotoxin-producing strains of Clostridium perfringens type A produced morphological changes in cells of 10 of 12 established mammalian cell lines tested, and particularly Vero (African green-monkey kidney) and MDCK (dog kidney) cells. Double-gel diffusion and Vero-cell tests detected enterotoxin in culture filtrates of, respectively, 29 (28%) and 56 (54%) of 103 strains of C. perfringens tested. The feasibility of cell-culture assays for the routine detection of C. perfringens enterotoxin is indicated.
CasmanE. P.,
BennettR. W.,
DorseyA. E.,
StoneJ. E.1969; The micro-slide gel double diffusion test for the detection and assay of staphylococcal enterotoxins. Health Laboratory Science 6:185–198
ColleeJ. G.1974; Clostridium perfringens (Cl. welchii) in the human gastro-intestinal tract. In The normal microbial flora of man edited by
SkinnerF. A.,
CarrJ. G.
Academic Press; London: pp 205–219
GigerO.,
ParizaM. W.1978; Depression of amino-acid transport in cultured rat hepatocytes by purified enterotoxin from Clostridium perfringens. Biochemical and Biophysical Research Communications 82:378–383
GilliganP. H.,
McCarthyL. R.,
GentaV. M.1981; Relative frequency of Clostridium difficile in patients with diarrheal disease. Journal of Clinical Microbiology 14:26–31
GiuglianoL. G.,
MannG. F.,
DrasarB. S.1982; The response of mammalian cell lines to toxins produced by Escherichia coli. Journal of Medical Microbiology 15:531–539
LabbeR. G.,
ReyD. K.1979; Raffinose increases sporulation and enterotoxin production by Clostridium perfringens type’ A. Applied and Environmental Microbiology 37:1196–1200
McClaneB. A.,
McDonelJ. L.1979; The effects of Clostridium perfringens enterotoxin on morphology, viability and macromolecular synthesis in Vero cells. Journal of Cellular Physiology 99:191–199
McDonelJ. L.,
McClaneB. A.1981; Highly sensitive assay for Clostridium perfringens enterotoxin that uses inhibition of plating efficiency of Vero cells grown in culture. Journal of Clinical Microbiology 13:940–946
SkjelkväleR.,
UemuraT.1977; Detection of enterotoxin in faeces and anti-enterotoxin in serum after Clostridium perfringens food poisoning. Journal of Applied Bacteriology 32:355–363
StringerM. F.,
WatsonG. N.,
GilbertR. J.1982; Clostridium perfringens type A: Serological typing and methods for the detection of enterotoxin. In Isolation and identification methods for food poisoning organisms edited by
CorryJ. E. L.RobertsD.,
SkinnerF. A.
Society for Applied Bacteriology Technical Series, No. 17 Academic Press; London: pp 111–135
TsaiC. C.,
Torres-AnjelM. J.,
RiemannH. P.1974; Improved culture techniques and sporulation medium for enterotoxin production by Clostridium perfringens type A. Journal of the Formosan Medical Association 73:404–409