RT Journal Article SR Electronic(1) A1 Giugliano, L. G. A1 Stringer, M. F. A1 Drasar, B. S.YR 1983 T1 Detection of Clostridium perfringens enterotoxin by tissue culture and double-gel diffusion methods JF Journal of Medical Microbiology, VO 16 IS 2 SP 233 OP 237 DO https://doi.org/10.1099/00222615-16-2-233 PB Microbiology Society, SN 1473-5644, AB SUMMARY Culture filtrates of four enterotoxin-producing strains of Clostridium perfringens type A produced morphological changes in cells of 10 of 12 established mammalian cell lines tested, and particularly Vero (African green-monkey kidney) and MDCK (dog kidney) cells. Double-gel diffusion and Vero-cell tests detected enterotoxin in culture filtrates of, respectively, 29 (28%) and 56 (54%) of 103 strains of C. perfringens tested. The feasibility of cell-culture assays for the routine detection of C. perfringens enterotoxin is indicated., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-16-2-233