Characteristics of motile curved rods in vaginal secretions Free

Abstract

SUMMARY

Motile curved rods seen in vaginal secretions have been isolated on Columbia agar supplemented with 5% human blood and vitamin K. Growth occurred anaerobically and in 5% oxygen but not in more aerobic conditions. There were two distinct groups of these organisms, distinguishable by morphology, biochemical activity and susceptibility to metronidazole. All isolates were sensitive to a wide range of antimicrobial agents, with the exception of nalidixic acid and polymyxin, but one group was resistant to metronidazole. There was little difference between the results of tests of susceptibility to aminoglycosides or to metronidazole performed in anaerobic and microaerophi-lic conditions. Motile curved rods were isolated from 18 of 80 patients with a clinical diagnosis of non-specific vaginitis, but from only two of 39 without the disease.

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1983-05-01
2024-03-28
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References

  1. Bondi A., Dietz C. C., Spaulding E. H. 1946; Interference with the antibacterial action of streptomycin by reducing agents. Science 103:399–401
    [Google Scholar]
  2. Cowan S. T. 1974; Cowan and Steel’s manual for the identification of medical bacteria. 2nd ed Cambridge University Press; Cambridge:
    [Google Scholar]
  3. Curtis A. H. 1913; A motile curved anaerobic bacillus in uterine discharges. Journal of Infectious Diseases 12:165–169
    [Google Scholar]
  4. Duerden B. I., Holbrook W. P., Collee J. G., Watt B. 1976; The characterization of clinically important gram negative anaerobic bacilli by conventional bacteriological tests. Journal of Applied Bacteriology 40:163–188
    [Google Scholar]
  5. Fontaine E.A., Taylor-Robinson D., Borriello S. P., Hanna N. F., Honour P. 1982; Anaerobic bacteria in lower genital-tract infections. Lancet 1:281
    [Google Scholar]
  6. Fuzi M., Csukas Z. 1970; Das antibakterielle Wirkungsspektrum des Metronidazols. Zentralblatt fur Bakteriologie, Parasitenkunde Infectionskrankheiten und Hygiene (Abteilung) 213:258–262
    [Google Scholar]
  7. Hjelm E., Hallen A., Forsum U., Wallin J. 1981; Anaerobic curved rods in vaginitis. Lancet 2:1353–1354
    [Google Scholar]
  8. Lowe G. H. 1962; The rapid detection of lactose fermentation in paracolon organisms by the demonstration of β-D-galactosidase. Journal of Medical Laboratory Technology 19:21–25
    [Google Scholar]
  9. Miles A. A., Misra S. S., Irwin J. O. 1938; The estimation of the bactericidal power of blood. Journal of Hygiene, Cambridge 38:732–749
    [Google Scholar]
  10. Moore B. 1954; Observations on a group of anaerobic vaginal vibrios. Journal of Pathology and Bacteriology 67:461–473
    [Google Scholar]
  11. Narang H. K., Codd A. A. 1979; A low-speed centrifugation technique for the preparation of grids for direct virus examination by electron microscopy. Journal of Clinical Pathology 32:304–305
    [Google Scholar]
  12. Narang H. K. 1982; Embedding bacteria and tissue culture cells for electron microscopy. Medical Laboratory Sciences 39:87–89
    [Google Scholar]
  13. Phillips I., Taylor E. 1982; Anaerobic curved rods in vaginitis. Lancet 1:221
    [Google Scholar]
  14. Prince H. N., Grunberg E., Titsworth E., Delorenzo W. F. 1969; Effects of 1 – (2-nitro-1-imidazolyl)-3-methoxy-2-propanol and 2-methyl-5-nitroimidazole-1-ethanol against anaerobic and aerobic bacteria and protozoa. Applied Microbiology 18:728–730
    [Google Scholar]
  15. Ralph E. D., Amatnieks Y. E. 1980; Relative susceptibilities of Gardnerella vaginalis (Haemophilus vaginalis), Neisseria gonorrhoeae and Bacteroides fragilis to metronidazole and its two major metabolites. Sexually Transmitted Diseases 7:157–160
    [Google Scholar]
  16. Rotimi V. O., Faulkner J., Duerden B. I. 1980; Rapid methods for identification of clinical isolates of Gram-negative anaerobic bacilli. Medical Laboratory Sciences 37:331–339
    [Google Scholar]
  17. Skarin A., Spiegel C. A., Westrom L., Holmes K. K., Mardh P. A. 1981; Demonstration of a strictly anaerobic, Gram-negative, comma-shaped bacterium in females with symptoms of lower genital tract infection. In Abstracts of 4th International Meeting on Sexually Transmitted Diseases (Heidelberg, October 18–20). p 9
    [Google Scholar]
  18. Skirrow M. B., Benjamin J. 1982; The classification of ‘thermophilic’ Campylobacters and their distribution in man and domestic animals. In Campylobacter Epidemiology, Pathogenesis and Biochemistry 1st ed., edited by Newel D. G. MTP Press Ltd.; Lancaster:
    [Google Scholar]
  19. Spiegel C. A., Skarin A., Mardh P. A., Holmes K. K. 1981; Isolation and characterisation of a curved motile anaerobic organism associated with non-specific vaginitis. In Abstracts of 4th International Meeting on Sexually Transmitted Diseases (Heidelberg, October 18–20). p 8
    [Google Scholar]
  20. Sprott M. S., Pattman R. S., Ingham H. R., Short G. R., Narang H. K., Selkon J. B. 1982; Anaerobic curved rods in vaginitis. Lancet 1:54
    [Google Scholar]
  21. Tanner A. C. R., Badger S., Lai C. H., Listgarten M. A., Visconti R. A., Socransky S. S. 1981; Wolinella gen. nov., Wolinella succinogenes (Vibrio succinogenes Wolin et al.) comb, nov., and Description of Bacteroides gracilis sp. nov., Wolinella recta sp. nov., Campylobacter conscisus sp. nov., and Eikenella corrodens from Humans with Periodontal Disease. International Journal of Systemic Bacteriology 31:432–445
    [Google Scholar]
  22. Taylor E., Blackwell A. L., Barlow D., Phillips I. 1982; Gardnerella vaginalis, anaerobes, and vaginal discharge. Lancet 1:1376–1379
    [Google Scholar]
  23. Thornley M. J. 1960; The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. Journal of Applied Bacteriology 23:37–52
    [Google Scholar]
  24. Verklin R. M., Mandell G. L. 1977; Alteration of effectiveness of antibiotics by anaerobiosis. Journal of Laboratory and Clinical Medicine 89:67–71
    [Google Scholar]
  25. Young H. 1978; Cultural diagnosis of gonorrhoea with modified New York City (MNYC) medium. British Journal of Venereal Diseases 54:36–40
    [Google Scholar]
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