1887

Abstract

SUMMARY

and were grown in Fastidious Anaerobe Broth with glucose concentrations from 0–1.5% (w/v). Gas chromatographic (GLC) analysis of fermentation end-products revealed that a change in glucose concentration markedly affected fermentation patterns of each microorganism. Increased glucose concentration resulted in stimulation of ethanol production by all strains, and of butanol and butyric acid by . With , there was a parallel increase in ethanol and acetic acid production and decreased formation of propanol and propionic, -butyric and -valeric acids. Increased ethanol formation by was accompanied by an increase in acetic acid and a decrease in butan-1-ol production. The greatest incremental change in GLC profiles occurred when glucose concentration was raised from zero to 0.1% (w/v). These data suggest that glucose concentration in a medium for GLC analysis must be rigorously controlled if reproducible results are to be obtained.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-16-1-61
1983-02-01
2022-05-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/16/1/medmicro-16-1-61.html?itemId=/content/journal/jmm/10.1099/00222615-16-1-61&mimeType=html&fmt=ahah

References

  1. Brooks J. B. 1977; Detection of bacterial metabolites in spent culture media and body fluids by electron capture gas-liquid chromatography. Advances in Chromatography 15:1–31
    [Google Scholar]
  2. Buckel W., Barker H. A. 1974; Two pathways of glutamate fermentation by anaerobic bacteria. Journal of Bacteriology 117:1248–1260
    [Google Scholar]
  3. Doelle H. W. 1969 Bacterial metabolism Academic Press; London: p 101
    [Google Scholar]
  4. Drucker D. B. 1981 Microbiological Applications of Gas Chromatography Cambridge University Press; Cambridge: pp 60–131
    [Google Scholar]
  5. Elsden S. R., Hilton M. G. 1978; Volatile acid production from threonine, valine, leucine and iso-leucine by Clostridia. Archives of Microbiology 117:165–172
    [Google Scholar]
  6. Ganguli L. A., Turton L. J., Tillotson G. S. 1982; Evaluation of Fastidious Anaerobe Broth as a blood culture medium. Journal of clinical Pathology 35:458–461
    [Google Scholar]
  7. Greenman J., Holland K. T., Cunliffe W. J. 1981; Effects of glucose concentration on biomass, maximum specific growth rate and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture. Journal of General Microbiology 127:371–376
    [Google Scholar]
  8. Hauser K. J., Zabransky R. J. 1976; Evaluation of the API anaerobe system (API 20A) for the identification of anaerobes in a clinical laboratory. Abstracts of the Annual Meeting of the American Society for Microbiology28
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe laboratory manual 4th ed VPI Anaerobe Laboratory; Blacksburg, VA:
    [Google Scholar]
  10. Lindner J. G. E. M., Marcelis J. H. 1978; Quantitative gas chromatography of Bacteroides species under different growth conditions. Antonie van Leeuwenhoek 44:1–14
    [Google Scholar]
  11. Loesche W. J., Gibbons R. J. 1968; Amino acid fermentation by Fusobacterium nucleatum. Archives of Oral Biology 13:191–201
    [Google Scholar]
  12. Lombard G. L. 1979; The effect of growth medium composition on the elaboration of metabolic acids detected by gas-liquid chromatography of anaerobic bacteria. Abstracts of the Annual Meeting of the American Society for Microbiology332
    [Google Scholar]
  13. McGilvery R. W. 1979 Biochemistry—a functional approach 2nd ed Holt-Saunders; Philadelphia: p 296
    [Google Scholar]
  14. Mayhew J. W., Gorbach S. L. 1975; Rapid gas chromatographic technique for presumptive detection of Clostridium botulinum in contaminated food. Applied Microbiology 29:297–299
    [Google Scholar]
  15. Shanson D. C. 1978; Blood culture techniques. In Modern topics in infection edited by Williams J. D. Heinemann; London: p 14
    [Google Scholar]
  16. Stadtman T. C. 1963; Anaerobic degradation of lysine. II. Cofactor requirements and properties of the soluble enzyme system. Journal of Biological Chemistry 238:2766–2773
    [Google Scholar]
  17. Wideman P. A., Vargo V. L., Citronbaum D., Finegold S. M. 1976; Evaluation of the sodium polyanethol sulphonate disc test for the identification of Peptostreptococcus anaerobius. Journal of Clinical Microbiology 4:330–333
    [Google Scholar]
  18. Wren M. W. D., Eldon C. P., Dakin G. H. 1977; Novobiocin and the differentiation of peptococci and peptostreptococci. Journal of Clinical Pathology 30:620–622
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-16-1-61
Loading
/content/journal/jmm/10.1099/00222615-16-1-61
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error