1887

Abstract

SUMMARY

In studies of the role of surface antigens of in HeLa cell invasion, three antisera were employed to block the invasion. Antisera against live (ALS) and boiled (ABS) blocked invasion very effectively. Reduction in the numbers of intracellular shigellae was always accompanied by reduction in the number adherent to the cells, indicating the importance of adhesion in the invasive process. Anti-live absorbed antiserum (ALAS) prepared by exhaustive absorption of ALS with boiled blocked adhesion and invasion at dilutions of 20 or 50; the efficiency of the absorption was indicated by absence of agglutinating and anti-lipopolysaccharide (LPS) antibodies. LPS did not block adhesion and invasion even at a concentration of 1.0 mg/ml. Hence it was concluded that heat-labile surface antigens are important in adhesion and invasion of HeLa cells by . Antiserum against heat stable antigen (ABS) probably blocks adhesion by steric hindrance.

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/content/journal/jmm/10.1099/00222615-16-1-37
1983-02-01
2024-04-18
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References

  1. Adamus G., Mulczyk M., Witkowska D., Romanowska E. 1980; Protection against keratoconjunctivitis shigellosa induced by immunization with outer membrane proteins of Shigella spp. Infection and Immunity 30:321–324
    [Google Scholar]
  2. Bancroft H. 1957 Introduction to Biostatistics, Harper and Row New York: p 172
    [Google Scholar]
  3. Edwards P. R., Ewing W. H. 1972; Identification of Enterobacteriaceae. 3rd ed Burgess Publishing Company, Minneapolis p 123
    [Google Scholar]
  4. Hale T. L., Bonventre P. F. 1979; Shigella infection of Henle intestinal epithelial cells: role of the bacterium. Infection and Immunity 24:879–886
    [Google Scholar]
  5. Hale T. L., Morris R. E., Bonventre P. F. 1979; Shigella infection of Henle intestinal epithelial cells: role of the host cell. Infection and Immunity 24:887–894
    [Google Scholar]
  6. Kwapinski J. B. G. 1972; Methology of Immunochemical and Immunological Research. Wiley Interscience New York: p 404
    [Google Scholar]
  7. Labrec E. H., Schneider H., Magnani T. J., Formal S. B. 1964; Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. Journal of Bacteriology 88:1503–1518
    [Google Scholar]
  8. Mackel D. C., Langley L. F., Venice L. A. 1961; Use of the guinea pig conjunctivae as an experimental model for the study of virulence of Shigella organisms. American Journal of Hygiene 73:219–223
    [Google Scholar]
  9. Okamura N., Nakaya R. 1977; Rough mutant of Shigella flexneri 2a that penetrates tissue culture cells but does not evoke keratoconjunctivitis in guinea pigs. Infection and Immunity 17:4–8
    [Google Scholar]
  10. Williams C. A., Chase M. W. 1977 Methods in Immunology and Immunochemistry Academic Press; New York: vol IV p 84
    [Google Scholar]
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