1887

Abstract

SUMMARY

Lancefield acid extracts of Streptococcus pyogenes, type 22 (T12, M22, OF positive) gave good yields of M protein and little opacity factor (OF), but sodium dodecyl sulphate (SDS) extracts contained high titres of OF (> 20 000) and little M protein. Acid-extracted OF could be separated from M protein by Sepharose 4B chromatography, but some of the OF-positive fractions that did not precipitate with the absorbed homologous anti-M rabbit serum, were able to neutralise opsonic antibodies present in human serum. The isoelectric-focusing profiles of the two antigens showed partial similarity.

Some strains of the OF-positive serotypes, e.g., M-types 22 and 49, lost both M antigen and OF activity on serial transfer in Todd-Hewitt broth, but this was not seen in a representative of M-type 60, and no M-negative OF-negative variants could be detected after six subcultures. Among the OF-negative serotypes some, e.g., M-types 5 and 6, were completely stable, whereas others, e.g., M-types 12,55 and 57, lost their M antigens after serial subculture. One explanation is that the genes that code for M antigen are plasmid borne in some serotypes and, moreover, are carried on the same plasmid as the gene for OF in some OF-positive serotypes. However, analysis of cell lysates by agarose-gel electrophoresis failed to demonstrate the presence of plasmid DNA in any of the strains tested.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-16-1-13
1983-02-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/16/1/medmicro-16-1-13.html?itemId=/content/journal/jmm/10.1099/00222615-16-1-13&mimeType=html&fmt=ahah

References

  1. Beachey E. H., Campbell G. L., Ofek I. 1974; Peptic digestion of streptococcal M protein. II Extraction of M antigen from group A streptococci with pepsin. Infection and Immunity 9:891–896
    [Google Scholar]
  2. Bradstreet C. M. P., Taylor C. E. D. 1962; Technique of complement-fixation test applicable to the diagnosis of virus diseases. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 21:96–104
    [Google Scholar]
  3. Chassy B.M. 1976; A gentle method for the lysis of oral streptococci. Biochemical and Biophysical Research Communications 68:603–608
    [Google Scholar]
  4. Cleary P. P. 1978; Genetic separation of serum opacity factor from M protein of group A streptococci. Infection and Immunity 22:171–175
    [Google Scholar]
  5. Cleary P. P., Johnson Z., Wannamaker L. W. 1975; Genetic instability of M protein and serum opacity factor of group A streptococci: evidence suggesting extrachromosomal control. Infection and Immunity 12:109–118
    [Google Scholar]
  6. Clewell D. G., Franke A. E. 1974; Characterization of a plasmid determining resistance to erythromycin, lincomycin and vernamycin Ba in a strain of Streptococcus pyogenes. Antimicrobial Agents and Chemotherapy 5:534–537
    [Google Scholar]
  7. Fischetti V. A., Gotschlich E. C., Siviglia G., Zabriskie J. B. 1976; Streptococcal M protein extracted by non-ionic detergent. 1. Properties of the antiphagocytic and type-specific molecules. Journal of Experimental Medicine 144:32–53
    [Google Scholar]
  8. Gooder H. 1961; Association of serum opacity reaction with serological type in Streptococcus pyogenes. Journal of General Microbiology 25:347–352
    [Google Scholar]
  9. Gooder H., Maxted W. R. 1958; Protoplasts of group A β-haemolytic streptococci. Nature 182:808–809
    [Google Scholar]
  10. Hallas G., Widdowson J. P. 1982; The opacity factor of group-A streptococci. Journal of Medical Microbiology 15: in press
    [Google Scholar]
  11. Havlicek J. 1975; M protein of type 12 Streptococcus pyogenes. Isolation by electro-focusing and some molecular weight-dependent properties. Pathologia et Microbiologia 42:147–158
    [Google Scholar]
  12. Klaenhammer T. R., McKay L. L., Baldwin K. A. 1978; Improved lysis of group N streptococci for isolation and rapid characterization of pasmid deoxyribonucleic acid. Applied and Environmental Microbiology 35:592–600
    [Google Scholar]
  13. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with Folin Phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. McCarty M. 1952; The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus. II. Nature of the cellular substrate attacked by the lytic enzymes. Journal of Experimental Medicine 96:569–580
    [Google Scholar]
  15. Maxted W. R., Potter E. V. 1967; The presence of type 12 M-protein antigen in Group G streptococci. Journal of General Micriobology 49:119–125
    [Google Scholar]
  16. Maxted W. R., Valkenburg H. A. 1969; Variation in the M-antigen of group-A streptococci. Journal of Medical Microbiology 2:199–210
    [Google Scholar]
  17. Maxted W. R., Widdowson J. P., Fraser C. A. M. 1973; Antibody to streptococcal opacity factor in human sera. Journal of Hygiene Cambridge: 71:35–42
    [Google Scholar]
  18. Maxted W. R., Widdowson J. P., Fraser C. A. M., Ball L. C., Bassett D. C. J. 1973; The use of the serum opacity reaction in the typing of group-A streptococci. Journal of Medical Microbiology 6:83–90
    [Google Scholar]
  19. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. 1976; Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology 127:1529–1537
    [Google Scholar]
  20. Pinney A. M., Widdowson J. P. 1977; Characteristics of the extracellular M proteins of group-A streptococci. Journal of Medical Microbiology 10:415–429
    [Google Scholar]
  21. Top F. H., Wannamaker L. W. 1968; The serum opacity reaction of Streptococcus pyogenes: frequency of production of streptococcal lipoproteinase by strains of different serological types and the relationship to M ‘protein production. Journal of Hygiene, Cambridge 66:49–58
    [Google Scholar]
  22. Totolian A. A. 1979; Transduction of M-protein and serum-opacity-factor production in group-A streptococci. In Pathogenic Streptococci edited by Parker M. T. Reedbooks Ltd; Chertsey, Surrey: pp 38–39
    [Google Scholar]
  23. Watanabe T., Ogata Y. 1970; Genetic stability of various resistance factors in Escherichia coli and Salmonella typhimurium. Journal of Bacteriology 102:363–368
    [Google Scholar]
  24. Widdowson J. P. 1980; The M-associated protein antigens of group A streptococci. In Streptococcal diseases and the immune response edited by Read S. E., Zabriskie J. B. Academic Press; New York: pp 125–147
    [Google Scholar]
  25. Widdowson J. P., Maxted W. R., Grant D. L. 1970; The production of opacity in serum by group A streptococci and its relationship with the presence of M antigen. Journal of General Microbiology 61:343–353
    [Google Scholar]
  26. Widdowson J.P., Maxted W. R., Grant D. L., Pinney A. M. 1971a; The relationship between M-antigen and opacity factor in group A streptococci. Journal of General Microbiology 65:69–80
    [Google Scholar]
  27. Widdowson J. P., Maxted W. R., Pinney A. M. 1971b; An M-associated protein antigen (MAP) of group A streptococci. Journal of Hygiene Cambridge: 69:553–564
    [Google Scholar]
  28. Widdowson J. P., Maxted W. R., Pinney A. M. 1976; Immunological heterogeneity among the M-associated protein antigens of group-A streptococci. Journal of Medical Microbiology 9:73–88
    [Google Scholar]
  29. Williams R. E. O., Maxted W. R. 1955; The type classification of Streptococcus pyogenes. Atti del VI Congresso Internazionale di Microbiologia Roma: 1:46–49
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-16-1-13
Loading
/content/journal/jmm/10.1099/00222615-16-1-13
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error