1887

Abstract

SUMMARY

strains resistant to metronidazole and tinidazole were isolated from the sensitive parent strain CM288 after mutagenesis with -methyl-′-nitro--nitrosoguanidine. Strain CM288 was already resistant to rifampicin and nalidixic acid; these genetic markers helped to confirm the identity of mutants. All mutants showed similar characteristics: they grew more slowly than the parent strain and failed to reach the same maximum turbidity; uptake of metronidazole and tinidazole from culture fluids was slow and end products of glucose metabolism were different from those of the parent. Pyruvate dehydrogenase activity was not detected in broken cell preparations of the mutant strains although this enzyme was readily detected in the parent strain. Changes in end products of glucose metabolism were consistent with the absence of pyruvate dehydrogenase activity because pyruvate was accumulated during growth and lactate levels were higher whereas acetate, CO2 and ethanol levels were diminished.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-15-4-503
1982-11-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/15/4/medmicro-15-4-503.html?itemId=/content/journal/jmm/10.1099/00222615-15-4-503&mimeType=html&fmt=ahah

References

  1. Britz M. L., Lowther D. A. 1981; Some properties of neutral proteinases from lysôsomes of rabbit polymorphonuclear leucocytes. Australian Journal of Experimental Biology and Medical Science 59:63–75
    [Google Scholar]
  2. Britz M. L., Wilkinson R. G. 1978a; Purification and properties of beta-lactamase from Bacteroides fragilis. Antimicrobial Agents and Chemotherapy 13:373–382
    [Google Scholar]
  3. Britz M. L., Wilkinson R. G. 1978b; Chloramphenicol acetyltransferase of Bacteroides fragilis. Antimicrobial Agents and Chemotherapy 14:105–111
    [Google Scholar]
  4. Britz M. L., Wilkinson R. G. 1979; Isolation and properties of metronidazole-resistant mutants of Bacteroides fragilis. Antimicrobial Agents and Chemotherapy 16:19–27
    [Google Scholar]
  5. Chien Y. W., Mizuba S. S. 1978; Activity-electroreduction relationship of antimicrobial metronidazole analogues. Journal of Medicinal Chemistry 21:374–380
    [Google Scholar]
  6. Dornbusch K., Nord C. E. 1974; In vitro effect of metronidazole and tinidazole on anaerobic bacteria. Medical Microbiology and Immunology 160:265–267
    [Google Scholar]
  7. Edwards D. I., Dye M., Carne H. 1973; The selective toxicity of antimicrobial nitroheterocyclic drugs. Journal of General Microbiology 76:135–145
    [Google Scholar]
  8. Grunberg E., Titsworth E. 1973; Chemotherapeutic properties of heterocyclic compounds: monocyclic compounds with five-membered rings. Annual Review of Microbiology 27:317–346
    [Google Scholar]
  9. Holdeman L. V., Moore W. E. C. 1975 Anaerobe laboratory manual. , 3rd. Virginia Polytechnic Institute and State University; Blacksburg, VA:
    [Google Scholar]
  10. Hunt P. S., Francis J. K., Peck G., Farrell K., Sali A. 1979; Tinidazole in the prevention of wound infection after elective colorectal surgery. Medical Journal of Australia 1:107–109
    [Google Scholar]
  11. Ings R. M. J., McFadzean J. A., Ormerod W. E. 1974; The mode of action of metronidazole in Trichomonas vaginalis and other micro-organisms. Biochemical Pharmacology 23:1421–1429
    [Google Scholar]
  12. Jayko L. G., Lichstein H. C. 1959; Nutritional factors concerned with growth and lecithinase production by Clostridium perfringens. Journal of Infectious Diseases 104:142–151
    [Google Scholar]
  13. LaRusso N. F., Tomasz M., Müller M., Lipman R. 1977; Interaction of metronidazole with nucleic acids in vitro. Molecular Pharmacology 13:872–882
    [Google Scholar]
  14. Lindmark D. G., Müller M. 1973; Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate Trichomonas foetus, and its role in pyruvate metabolism. Journal of Biological Chemistry 248:7724–7728
    [Google Scholar]
  15. Lowry O. H., Rosenbrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153:375–380
    [Google Scholar]
  17. O’Brien R. W., Morris J. G. 1972; Effect of metronidazole on hydrogen production by Clostridium acetobutylicum. Archiv für Mikrobiologie 84:225–233
    [Google Scholar]
  18. Populaire P., DeCouvelaere B., Lebreton G., Pascal S. 1968; Dosage colorimétrique de l (hydroxy-2 éthyl)-1 méthyl-2 nitro-5 imidazole (metronidazole). Annales Pharmaceutiques Françaises 26:549–556
    [Google Scholar]
  19. Rood J. L, Scott V. N., Duncan C. L. 1978; Identification of a transferable tetracycline resistance plasmid (p CW3) from Clostridium perfringens. Plasmid 1:563–570
    [Google Scholar]
  20. Tally F. P., Goldin B. R., Sullivan N., Johnston J., Gorbach S. L. 1978; Antimicrobial activity of metronidazole in anaerobic bacteria. Antimicrobial Agents and Chemotherapy 13:460–465
    [Google Scholar]
  21. Tanowitz H. B., Wittner M., Rosenbaum R. M., Kress Y. 1975; In vitro studies on the differential toxicity of metronidazole in protozoa and mammalian cells. Annals of Tropical Medicine and Parasitology 69:19–28
    [Google Scholar]
  22. Thompson A. R. 1951; Separation of saturated mono-hydroxamic acids by partition chromatography on paper. Australian Journal of Scientific Researchseries B 4:180–186
    [Google Scholar]
  23. Valentine R. C. 1964; Bacterial ferredoxin. Bacteriological Reviews 28:497–517
    [Google Scholar]
  24. Willis A. T. 11 others 1975; An evaluation of metronidazole in the prophylaxis and treatment of anaerobic infections in surgical patients. Journal of Antimicrobial Chemotherapy 1:393–401
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-15-4-503
Loading
/content/journal/jmm/10.1099/00222615-15-4-503
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error