The Action of Cells from Patients with Chronic Granulomatous Disease on Free

Abstract

SUMMARY

Contrary to current opinion, neutrophil leucocytes from patients with the inherited immunodeficiency syndrome chronic granulomatous disease (CGD) killed 80% of ingested Bacterial killing was not impaired by increasing the ratio of bacteria to cells from 1:1 to 10:1. The organisms that survived within the patients' cells did not themselves appear to constitute an unduly resistant subpopulation because they were killed when exposed to fresh cells, and no growth phase of a synchronous culture was found to be particularly resistant. The H within the phagocytic vacuoles of CGD neutrophils and monocytes is abnormally low and methylamine, which has been shown to normalise this vacuolar H, improved killing. Clumped bacteria appeared to be more resistant to killing than dispersed ones, suggesting that organisms near the centre of a clump might be protected from the toxicity of the compromised killing systems in cells of these patients.

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/content/journal/jmm/10.1099/00222615-15-4-441
1982-11-01
2024-03-28
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References

  1. Armitage P. 1971 Statistical methods in medical research. Blackwell; Oxford:281–282289–291
    [Google Scholar]
  2. Babior B. M. 1978; Oxygen dependent microbial killing by phagocytes. New England Journal of Medicine 298:721–725
    [Google Scholar]
  3. Baldridge C. W., Gerard R. W. 1933; The extra respiration of phagocytosis. American Journal of Physiology 103:235–236
    [Google Scholar]
  4. Chatterjee A. N., Taber H., Young F. E. 1971; A rapid method for synchronization of Staphylococcus aureus and Bacillus subtilis. Biochemical and Biophysical Research Communications 44:1125–1130
    [Google Scholar]
  5. Holmes B., Page A. R., Good R. A. 1967; Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function. Journal of clinical Investigation 46:1422–1432
    [Google Scholar]
  6. Klebanoff S. J. 1975; Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes. Seminars in Hematology 12:117–142
    [Google Scholar]
  7. McRipley R. J., Sbarra A. J. 1967; Role of the phagocyte in host-parasite interactions. XI. Relationship between stimulated oxidative metabolism and hydrogen peroxide formation, and intracellular killing. Journal of Bacteriology 94:1417–1424
    [Google Scholar]
  8. Mandell G. L. 1974; Bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils. Infection and Immunity 9:337–341
    [Google Scholar]
  9. Mandell G. L. 1975; Catalase, superoxide dismutase and virulence of Staphylococcus aureus. In vitro and in vivo studies with emphasis on staphylococcal-leukocyte interaction. Journal of clinical Investigation 55:561–566
    [Google Scholar]
  10. Mandell G. L., Hook E. W. 1969a; Leukocyte function in chronic granulomatous disease of childhood. Studies on a seventeen year old boy. American Journal of Medicine 47:473–486
    [Google Scholar]
  11. Mandell G. L., Hook E. W. 1969b; Leukocyte bactericidal activity in chronic granulomatous disease: correlation of bacterial hydrogen peroxide production and susceptibility to intracellular killing. Journal of Bacteriology 100:531–532
    [Google Scholar]
  12. Mitchison J. M., Vincent W. S. 1965; Preparation of synchronous cell cultures by sedimentation. Nature (London 205:987–989
    [Google Scholar]
  13. Odeberg H., Olsson I. 1975; Anti-bacterial activity of cationic proteins from human granulocytes. Journal of clinical Investigation 56:1118–1124
    [Google Scholar]
  14. Quie P. G., White J. G., Holmes B., Good R. A. 1967; vitro bactericidal capacity of human polymorphonuclear leukocytes: diminished activity in chronic granulomatous disease of childhood. Journal of clinical Investigation 46:648–649
    [Google Scholar]
  15. Segal A. W., Geisow M., Garcia R., Harper A., Miller R. 1981; The respiratory burst of phagocytic cells is associated with a rise in vacuolar pH. Nature (London 290:406–409
    [Google Scholar]
  16. Segal A. W., Jones O. T. G. 1980; Absence of cytochrome b reduction in stimulated neutrophils from both female and male patients with chronic granulomatous disease. FEBS Letters 110:111–114
    [Google Scholar]
  17. Selvaraj R. J., Sbarra A. J. 1966; Relationship of glycolytic and oxidative metabolism to particle entry and destruction in phagocytosing cells. Nature (London), 111,1272–1276
    [Google Scholar]
  18. Tan J. S., Watanakunakorn C., Phair J. P. 1971; A modified assay of neutrophil function: use of lysostaphin to differentiate defective phagocytosis from impaired intracellular killing. Journal of laboratory and clinical Medicine 78:316–322
    [Google Scholar]
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