1887

Abstract

SUMMARY

Various strains of were compared by bacterial restriction-endonuclease DNA analysis (BRENDA). Field strains of serovar isolated from domestic animals in New Zealand, Australia and Northern Ireland were indistinguishable from one another but differed strikingly from the reference strain Hardjoprajitno. Similarly, field isolates of and differed from their serovar reference strains, probably owing to a difference in epidemiological niche. Subdivision of these serovars into distinct subtypes as defined by BRENDA is therefore useful and justified. In contrast, analysis of serovars and shows that field and reference strains were identical, or differed only by a single band.

It is suggested that BRENDA will overcome many of the problems associated with serological methods of identifying serovars and allow more precise definition of epidemiological relationships between strains and their hosts.

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1982-08-01
2022-08-18
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References

  1. Babudieri B., Mateev D. 1961; Studies serologico di alcuni ceppi bulgari di leptospire. Rendiconti dell’Istituto Superiori di Sanità 24:614–622
    [Google Scholar]
  2. Marshall R. B., Wilton B. E., Robinson A. J. 1981; Identification of leptospira serovars by restriction-endonuclease analysis. Journal of Medical Microbiology 14:163–166
    [Google Scholar]
  3. Matveeva A. A., Sakharova P. U., Sharban E. K., Dragomir A. V. 1977; Etiologiya leptospiroza u zhivotnukh. Veterinariya 1:61–63
    [Google Scholar]
  4. Miller J. H. 1972 Experiments in molecular genetics. Cold Spring Harbour Laboratories; New York:331
    [Google Scholar]
  5. Semenova L. P., Soloshenko I. Z., Ananin V. V. 1965; Leptospiry gruppy Hebdomadis. III. Obnaruzhenie V Sovetskom Soyuze podtipa L. sejroe balcanica. Zhurnal Mikrobiolo-gii, Epidemiologii i Immunobiologii 42:461–64
    [Google Scholar]
  6. Tanaka T., Weisblum B. 1975; Construction of a colicin El-R Factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. Journal of Bacteriology 121:354–362
    [Google Scholar]
  7. Wolff J. W. 1953 The classification of pathogenic leptospires. Walter Reed Army Medical Center and Army Medical Service Graduate School Symposium on the leptospires Dec. 1952, Medical Science Publication no. 1 US Government Printing Office; Washington:174–185
    [Google Scholar]
  8. Younghusband H. B., Bellett A. J. D. 1971; Mature form of the deoxyribonucleic acid from chick embryo lethal orphan virus. Journal of Virology 8:265–274
    [Google Scholar]
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