1887

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Volume 15, Issue 3

Virulence of in Ascending Urinary-tract Infection in Mice Free

Abstract

SUMMARY

The virulence of strains in ascending urinary-tract infection was studied in mice drinking a 5% glucose solution; factors determining the virulence were examined. Of 33 strains, 8 (group I) infected the bladder and kidney, 10 (group II) infected only the bladder, while the remaining 15 strains (group III) did not cause infection. The adherence of group-I and group-II strains to bladder epithelial cells in vitro was inhibited by D-mannose. In group III, 13 strains barely adhered to the epithelial cells, while two strains showed an adherence unaffected by D-mannose. Most strains in groups I and II agglutinated erythrocytes of guinea-pig, chicken, and horse, and cells of in a mannose-sensitive manner. All strains in groups I and II had fimbriae. Virulence for the urinary tract was not directly related with O-serotype, intraperitoneal virulence, ability to grow in mouse urine, ability to ferment dulcitol, production of haemolysin, susceptibility to serum bactericidal activity, or susceptibility to antibiotics. These results suggest that the adherence of the to mouse-bladder epithelial cells in a mannose-sensitive manner plays an important role in the development of urinary-tract infection in mice and that the adherence is probably mediated by type-1 or closely related fimbriae.

  • Received:
  • Accepted:
  • Published Online:
© 1982 Pathological Society of Great Britain and Ireland
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1982-08-01
2022-08-18
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References

  1. Aronson M., Medalia O., Schori L., Mirelman D., Sharon N., Ofek I. 1979; Prevention of colonization of the urinary tract of mice with Escherichia coli by blocking of bacterial adherence with methyl a-D-mannopyranoside. Journal of Infectious Diseases 139:329–332
     [Google Scholar]
  2. Behrens B., Kärber G. 1935; Wie sind Reihenversuche für biologische Auswertungen am zweckmässigsten anzuorden?. Archives of Experimental Pathology and Pharmacology 177:379–388
     [Google Scholar]
  3. Björksten B., Kaijser B. 1978; Interaction of human serum and neutrophils with Escherichia coli strains; differences between strains isolated from urine of patients with pyelonephritis or asymptomatic bacteriuria. Infection and Immunity 22:308–311
     [Google Scholar]
  4. Brumfitt W., Heptinstall R. H. 1960; Experimental pyelonephritis; the relationship of bacterial virulence to the establishment of the renal lesion. British Journal of Experimental Pathology 41:552–558
     [Google Scholar]
  5. Cooke E. M. 1968; Properties of strains of Escherichia coli isolated from the faeces of patients with ulcerative colitis, patients with acute diarrhoea and normal persons. Journal of Pathology and Bacteriology 95:101–113
     [Google Scholar]
  6. Cooke E. M., Ewins S. P. 1975; Properties of strains of Escherichia coli isolated from a variety of sources. Journal of Medical Microbiology 8:107–111
     [Google Scholar]
  7. Cotran R. S. 1969 Experimental pyelonephritis. The kidney 2 edited by Rouiller C., Muller A. F. Academic Press; New York:269–361
     [Google Scholar]
  8. Duguid J. P., Old D. C. 1980 Adhesive properties of enterobacteriaceae. Bacterial adherence edited by Beachey E. H. Chapman and Hall; London:185–217
     [Google Scholar]
  9. Fader K. C, Avots-Avotins A. E., Davis C. P. 1979; Evidence for pili-mediated adherence of Klebsiella pneumoniae rat bladder epithelial cells in vitro. Infection and Immunity 25:729–737
     [Google Scholar]
  10. Fader R. C., Davis C. P. 1980; Effect of piliation on Klebsiella pneumoniae infection in rat bladders. Infection and Immunity 30:554–561
     [Google Scholar]
  11. Fried F. A., Vermeulen C. W., Ginsburg M. J., Cone C. M. 1971; Etiology of pyelonephritis: further evidence associating the production of experimental pyelonephritis with hemolysis in Escherichia coli. Journal of Urology 106:351–354
     [Google Scholar]
  12. Glynn A. A., Brumfitt W., Howard C. J. 1971; K antigens of Escherichia coli and renal involvement in urinary tract infections. Lancet 1:514–516
     [Google Scholar]
  13. Guze L.B., Montgomery J. Z., Potter C. S., Kalmanson G. M. 1973; Pyelonephritis. XVI. Correlates of parasite virulence in acute ascending Escherichia coli pyelonephritis in mice undergoing diuresis. Yale Journal of Biology and Medicine 46:203–211
     [Google Scholar]
  14. Kaijser B. 1973; Immunology of Escherichia coli: K antigen and its relation to urinary-tract infection. Journal of Infectious Diseases 127:670–677
     [Google Scholar]
  15. Kaijser B., Hanson L. A., Jodal U., Lidin-Janson G., Robbins J. B. 1977; Frequency of £. coli K antigens in urinary tract infections in children. Lancet 1:663–664
     [Google Scholar]
  16. Keane W. F., Freedman L. R. 1967; Experimental pyelonephritis. XIV. Pyelonephritis in normal mice produced by inoculation of E. coli into the bladder lumen during water diuresis. Yale Journal of Biology and Medicine 40:231–237
     [Google Scholar]
  17. Kimball H., Turck M., Petersdorf R. G. 1964; The epidemiology of nonenteric Escherichia coli infections. III. Relationship of E. coli antigens to experimental pyelonephritis. Journal of Laboratory and Clinical Medicine 63:907–913
     [Google Scholar]
  18. Källenius G., MÖLLBY R., Winberg J. 1980; vitro adhesion of uropathogenic Escherichia coli to human periurethral cells. Infection and Immunity 28:972–980
     [Google Scholar]
  19. Korhonen T. K., Leffler H., Svanborg Edên C. 1981; Binding specificity of piliated strains of Escherichia coli and Salmonella typhimurium to epithelial cells, Saccharomyces cerevisiae cells, and erythrocytes. Infection and Immunity 32:796–804
     [Google Scholar]
  20. McCabe W. R., Carling P. C, Bruins S., Greely A. 1975; The relation of K antigen to virulence of Escherichia coli. Journal of Infectious Diseases 131:6–10
     [Google Scholar]
  21. Minshew B. H., Jorgensen J., Swanstrum M., Grootes-Reuvecamp G. A., Falkow S. 1978; Some characteristics of Escherichia coli strains isolated from extraintestinal infections of humans. Journal of Infectious Diseases 137:648–654
     [Google Scholar]
  22. Montgomerie J. Z. 1978; Factors affecting virulence in Escherichia coli urinary tract infections. Journal of Infectious Diseases 137:645–647
     [Google Scholar]
  23. Nicholson A. M., Glynn A. A. 1975; Investigation of the effect of K antigen in Escherichia coli urinary tract infections by use of a mouse model. British Journal of Experimental Pathology 56:549–553
     [Google Scholar]
  24. Ofek I., Beachey E. H. 1978; Mannose binding and epithelial cell adherence of Escherichia coli. Infection and Immunity 22:247–254
     [Google Scholar]
  25. Ofek I., Beachey E. H. 1980 General concepts and principles of bacterial adherence in animals and man. Bacterial adherence edited by Beachey E. H. Chapman and Hall; London:1–29
     [Google Scholar]
  26. Olling S. 1977; Sensitivity of gram-negative bacilli to the serum of bactericidal activity: a marker of the host parasite relationship in acute and persisting infections. Scandinavian Journal of Infectious Diseasessupplement 101–40
     [Google Scholar]
  27. øRSKOV I., øRSKOV F., Birch-Andersen A. 1980; Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae. Infection and Immunity 27:657–666
     [Google Scholar]
  28. Prat V., Hatala M., Benesova D., Rossmann P. 1965 Pathogenicity of various strains of Escherichia coli for the intact rabbit kidney and the effect of repeated passage on renal tissue. Progress in pyelonephritis edited by Kass E. H. Davis; Philadelphia:135–146
     [Google Scholar]
  29. Salit I. E., Gotschlich E. C. 1977a; Hemagglutination by purified type 1 Escherichia coli pili. Journal of Experimental Medicine 146:1169–1181
     [Google Scholar]
  30. Salit I. E., Gotschlich E. C. 1977b; Type 1 Escherichia coli pili: characterization of binding to monkey kidney cells. Journal of Experimental Medicine 146:1182–1194
     [Google Scholar]
  31. Schaeffer A. J., Amundsen S. K., Schmidt L. N. 1979; Adherence of Escherichia coli to human urinary tract epithelial cells. Infection and Immunity 24:753–759
     [Google Scholar]
  32. Svanborg Edên C. 1978; Attachment of Escherichia coli to human urinary tract epithelial cells. Scandinavian Journal of Infectious Diseasessupplement 151–6
     [Google Scholar]
  33. Svanborg Edên C, Hanson L. A., Jodel U., Lindberg U., Akerlund A. S. 1976; Variable adherence to normal human urinary tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection. Lancet 2:490–492
     [Google Scholar]
  34. Tsuchiya K. seven others 1981; Cefmenoxime (SCE-1365), a novel broad-spectrum cephalosporin: in vitro and in vivo antibacterial activities. Antimicrobial Agents and Chemotherapy 19:56–65
     [Google Scholar]
  35. Turck M., Petersdorf R. G., Fournier M. R. 1962; The epidemiology of non-enteric Escherichia coli infections: prevalence of serological groups. Journal of Clinical Investigation 41:1760–1765
     [Google Scholar]
  36. van den Bosch J. F., de Graaff J., MacLaren D. M. 1979; Virulence of Escherichia coli in experimental hematogenous pyelonephritis in mice. Infection and Immunity 25:68–74
     [Google Scholar]
  37. van den Bosch J. F., Verboom-Sohmer U., Postma P., de Graff J., MacLaren D. M. 1980; Mannose-sensitive and mannose-resistant adherence to human uroepithelial cells and urinary virulence of Escherichia coli. Infection and Immunity 29:226–233
     [Google Scholar]
  38. Vosti K. L., Goldberg L. M., Monto A. S., Rantz L. A. 1964; Host-parasite interaction in patients with infections due to Escherichia coli. I. The serogrouping of E. coli from intestinal and extraintestinal sources. Journal of Clinical Investigation 43:2377–2385
     [Google Scholar]
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Virulence of Escherichia Coli in Ascending Urinary-tract Infection in Mice
J. Med. Microbiol. 15, 303 (1982); https://doi.org/10.1099/00222615-15-3-303
/content/journal/jmm/10.1099/00222615-15-3-303
/content/journal/jmm/10.1099/00222615-15-3-303
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