1887

Abstract

SUMMARY

The preliminary characterisation of an unusual gram-negative bacillus isolated from genital ulcers in Swaziland is reported. Like , it is an oxidase positive, nitrate-reductase-positive gram-negative rod that forms streptobacillary chains in some circumstances; it was therefore called the “ducreyi-like bacterium” (DLB). Distinguishing features of DLB are production of α-haemolysis on horse-blood agar, stimulation of growth by a microaerophilic atmosphere and by a factor produced by , a strongly positive porphyrin test, and a remarkable ability to undergo autolysis. DLB has a guanine + cytosine value of . 50 mole% but it cannot be classified, even at the genus level, until more taxonomic data are obtained.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-15-1-97
1982-02-01
2024-10-09
Loading full text...

Full text loading...

/deliver/fulltext/jmm/15/1/medmicro-15-1-97.html?itemId=/content/journal/jmm/10.1099/00222615-15-1-97&mimeType=html&fmt=ahah

References

  1. Aoki Y., Kawai K., Nakamura T., Shiraki M., Harada H. 1952; An organism resembling Hemophilus ducreyi; a species found at a high rate in genitals of prostitutes in the Sasebo area. Yokohama med. Bull. 3:310
    [Google Scholar]
  2. Brown W. J. 1974; Modification of the rapid fermentation test for Neisseria gonorrhoeae. Appl. Microbiol. 27:1027
    [Google Scholar]
  3. Chapel T., Brown W. J., Jeffries C., Stewart J. A. 1978; The microbiological flora of penile ulcerations. J. infect. Dis. 137:50
    [Google Scholar]
  4. Clarke P. H., Cowan S. T. 1952; Biochemical methods for bacteriology. J. gen. Microbiol. 6:187
    [Google Scholar]
  5. Deacon W. E., Albritton D. C, Olansky S., Kaplan W. 1956; V.D.R.L. chancroid studies. I. A simple procedure for the isolation and identification of Haemophilus ducreyi. J. invest. Derm. 26:399
    [Google Scholar]
  6. De ley J. 1970; Reexamination of the association between melting point, buoyant density and chemical base composition of deoxyribonucleic acid. J. Bact. 101:738
    [Google Scholar]
  7. De Ley J., van Muylem J. 1963; Some applications of deoxyribonucleic acid base composition in bacterial taxonomy. Antonie van Leeuwenhoek 29:344
    [Google Scholar]
  8. Hammond G. W., Lian C. J., Wilt J. C., Ronald A. R. 1978; Comparison of specimen collection and laboratory techniques for the isolation of Haemophilus ducreyi. J. clin Microbiol 7:39
    [Google Scholar]
  9. Kampmeier R. H. 1977 Chancroid. In Infectious diseases, 2nd. edited by Hoeprich P. D. Harper & Row; Hagerstown, Md:507
    [Google Scholar]
  10. Kilian M. 1974; A rapid method for the differentiation of Haemophilus strains. The porphyrin test. Acta path, microbiol. scand. B 82:835
    [Google Scholar]
  11. Kilian M. 1976; A taxonomie study of the genus Haemophilus, with the proposal of a new species. J. gen. Microbiol. 93:9
    [Google Scholar]
  12. Kovâcs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature, Lond. 178:703
    [Google Scholar]
  13. Leibovitz A. 1954; An outbreak of pyogenic penile ulcers associated with a microaerophilic streptococcus resembling Haemophilus ducreyi. Am. J. Syph. Neurol. 38:203
    [Google Scholar]
  14. Le Minor L., Ben Hamida F. 1962; Avantages de la recherche de la β-galactosidase sur celle de la fermentation du lactose en milieu complexe dans le diagnostic bactériologique, en particulier des Enterobacteriaceae. Annls Inst. Pasteur, Paris 102:267
    [Google Scholar]
  15. Marmur J. A. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. molec. Biol. 3:208
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Molec. Biol. 5:109
    [Google Scholar]
  17. Ryter A., Kellenberger E. 1958; Etude au microscope électronique de plasmas contenant de l’acide désoxyribonucléique. 1. Les nucleotides des bactéries en croissance active. Z. Naturf. 13B:597
    [Google Scholar]
  18. Shaw C., Clarke P. H. 1955; Biochemical classification of Proteus and Providence cultures. J. gen. Microbiol. 13:155
    [Google Scholar]
  19. Spurr A.R. 1969; A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26:31
    [Google Scholar]
  20. White M. L., Pickett M. J. 1953; A rapid phosphatase test for Micrococcus pyogenes var. aureus for detection of potentially pathogenic strains. Am J. clin. Path. 23:1181
    [Google Scholar]
  21. Zinneman K., Bibersteon E. L. 1974 Genus Haemophilus. Bergey’s manual of determinative bacteriology, 8th. edited by Buchanan R. E., Gibbons N. E. Williams & Wilkins; Baltimore:364
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-15-1-97
Loading
/content/journal/jmm/10.1099/00222615-15-1-97
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error