RT Journal Article SR Electronic(1) A1 Askelöf, P. A1 Granström, Marta A1 Gillenius, P. A1 Lindberg, A. A.YR 1982 T1 Purification and Characterisation of a Fimbrial Haemagglutinin from Bordetellapertussis for Use in an Enzyme-linked Immunosorbent Assay JF Journal of Medical Microbiology, VO 15 IS 1 SP 73 OP 83 DO https://doi.org/10.1099/00222615-15-1-73 PB Microbiology Society, SN 1473-5644, AB SUMMARY The fimbrial haemagglutinin (F-HA) of Bordetella pertussis grown on solid medium was extracted with 1m sodium acetate for 72 h at 20°C, and partially purified by Sephacryl S-300 gel chromatography. A pooled fraction with fimbrial haemagglutinating activity was shown to contain fimbriae of the expected morphology by electron microscopy. Chemical and biological assays showed that the F-HA fraction contained some heat-labile agglutinogen and lipopolysaccharide but no measurable lymphocytosis-promoting factor or heat-labile toxin. The F-HA fraction used as antigen in an enzyme-linked immunosorbent assay (ELISA) permitted the detection of antibodies in convalescent serum from a patient with whooping cough. The impurities, heat-labile agglutinogens and lipopolysaccharide, did not contribute to the ELISA activity. The method for preparation of the F-HA antigen is simple, reproducible and gives a high yield., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-15-1-73