1887

Abstract

SUMMARY

Three recently described methods for quantitative sampling of the bacterial flora of the vagina were evaluated and none proved satisfactory. In a third of the samples, paired swabs showed large differences between the two weights of vaginal secretion collected by this method, and the recovery rate of bacteria deliberately added to test swabs was unsatisfactorily low. A calibrated loop gave a wide variation in the amount of secretion collected, due to variations in density and viscosity of the secretion. When secretion was collected with a calibrated pipette, it was often difficult to expel the collected volume from the pipette for testing. A simple weight-based method was devised in which a loop was used to collect an undefined volume of secretion for weighing in a tube of transport medium before homogenisation and quantitative bacteriological testing. Initial assessment indicates this to be a satisfactory method for quantitative studies of the vaginal bacterial flora.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-15-1-141
1982-02-01
2022-08-14
Loading full text...

Full text loading...

/deliver/fulltext/jmm/15/1/medmicro-15-1-141.html?itemId=/content/journal/jmm/10.1099/00222615-15-1-141&mimeType=html&fmt=ahah

References

  1. Bartlett J. G., Onderdonk A. B., Drude E., Goldstein C, Anderka M., Alpert S., McCormack W. M. 1977; Quantitative bacteriology of the vaginal flora. J. infect. Dis. 136:271
    [Google Scholar]
  2. Clarke R. T. J. 1977 In The microbial ecology of the gut edited by Clarke R. T. J., Bauchop T. London: Academic Press;1
    [Google Scholar]
  3. Collee J. G., Watt B., Brown R., Johnstone S. 1974; The recovery of anaerobic bacteria from swabs. J. Hyg., Camb. 72:339
    [Google Scholar]
  4. Cowan S. T. 1974 Cowan and Steel’s Manual for the identification of medical bacteria. , 2nd. Cambridge University Press; Cambridge:
    [Google Scholar]
  5. Galask R. P., Larsen B., Ohm M. J. 1976; Vaginal flora and its role in disease entities. Clin. Obstets Gynaec 19:61
    [Google Scholar]
  6. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe laboratory manual. , 4th. Virginia Polytechnic Institute and State University; Blacksburg, Virginia:
    [Google Scholar]
  7. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bact. Rev. 14:1
    [Google Scholar]
  8. Levison M. E., Corman L. C, Carrington E. R., Kaye D. 1977; Quantitative microflora of the vagina. Am. J. Obstet. Gynec 127:80
    [Google Scholar]
  9. Lindner J. G. E. M., Plantema F. H. F., Hoogkamp-Korstanje J. A. A. 1978; Quantitative studies of the vaginal flora of healthy women and of obstetric and gynaecological patients. J. med. Microbiol. 11:233
    [Google Scholar]
  10. Onderdonk A. B., Polk B. F., Moon N. E., Goren B., Bartlett J. G. 1977; Methods for quantitative vaginal flora studies. Am. J. Obstet. Gynec 128:777
    [Google Scholar]
  11. Riddell R.H., Buck A. C. 1970; Trimethoprim as an additional selective agent in media for the isolation of N. gonorrhoeae. J. clin. Path. 23:481
    [Google Scholar]
  12. Sutter V. L., Vargo V. L., Finegold S. M. 1975 Wadsworth anaerobic bacteriology manual. , 2nd. University Extension and School of Medicine U.C.L.A.; Los Angeles:
    [Google Scholar]
  13. Tabaqchali S. 1970; The pathophysiological role of small intestinal bacterial flora. Scand.J. Gastroenterol. 5:suppl. 6139
    [Google Scholar]
  14. Tabaqchali S., Fiddian A. P., Atkinson P. 1979; Recent techniques in the investigation and diagnosis of anaerobic infections. J. Infect. 1:suppl. 113
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-15-1-141
Loading
/content/journal/jmm/10.1099/00222615-15-1-141
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error