1887

Abstract

SUMMARY

An indirect enzyme-linked immunosorbent assay (ELISA) with a mixture of eight different gonococcal outer-membrane proteins (OMP) as coating antigen was evaluated for detection of gonococcal antibody in 507 sera obtained from patients selected from high-risk and low-risk populations. The indirect ELISA method was more specific and sensitive when the polyvalent antigen was used than when OMP from only one serotype was used. Past episodes of gonorrhoea had a significant influence on the seropositivity of the test. In a selected low-risk population the specificity of the assay was 94% and in a selected high-risk population the sensitivity was 78%. When sera from 24 asymptomatic individuals were tested the sensitivity was 83%. The ELISA polyvalent-antigen test should be useful as an aid for the detection of gonorrhoea in populations with a low prevalence.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-15-1-1
1982-02-01
2022-08-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/15/1/medmicro-15-1-1.html?itemId=/content/journal/jmm/10.1099/00222615-15-1-1&mimeType=html&fmt=ahah

References

  1. Armstrong A. S., Mathias J. R., De Young M. I., Hirata A. A. 1979; Strain differentiation of Neisseria gonorrhoeae by reverse passive hemagglutination. Infect. Immun. 24:51
    [Google Scholar]
  2. Brodeur B. R., Ashton F. E., Diena B. B. 1978; Enzyme-linked immunosorbent assays for the detection of Neisseria gonorrhoeae specific antibodies. Can. J. Microbiol. 24:1300
    [Google Scholar]
  3. Brodeur B. R., Johnson W. M., Diena B. B., Visentin L. P., Johnson K. G. 1977; Mitogenic activity of Neisseria gonorrhoeae surface antigens in mouse splenic lymphocyte culture. Can. J. Microbiol. 23:1154
    [Google Scholar]
  4. Buchanan T. M., Swanson J., Holmes K. K., Kraus S. J., Gotschlich E. C. 1973; Quantitative determination of antibody to gonococcal pili. Changes in antibody levels with gonococcal infection. J. clin. Invest. 52:2896
    [Google Scholar]
  5. Gaafar H. A. 1979; Serologic diagnosis of gonococcal infection. Archs Androl. 3:337
    [Google Scholar]
  6. Glynn A. A., Ison C. 1978; Serological diagnosis of gonorrhoea by an enzyme-linked immunosorbent assay (ELISA). Br. J. vener. Dis. 54:97
    [Google Scholar]
  7. Holmes K. K., Buchanan T. M., Adam J. L., Eschenbach D. A. 1978 Is serology useful in gonorrhea? A critical analysis of factors influencing serodiagnosis. In Immunobiology of Neisseria gonorrhoeae edited by Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young F. E. American Society of Microbiology; Washington DC:370
    [Google Scholar]
  8. Johnston K. H. 1980; Antigenic diversity of the serotype antigen complex of Neisseria gonorrhoeae: analysis by an indirect enzyme-linked immunoassay. Infect. Immun. 28:101
    [Google Scholar]
  9. Johnston K. H., Holmes K. K., Gotschlich E. C. 1976; The serological classification of Neisseria gonorrhoeae. I. Isolation of the outer membrane complex responsible for serotypic specificity. J. exp. Med. 143:741
    [Google Scholar]
  10. Oates S.A., Falkler W.A., Joseph J. M., Warfel L.E. 1977; Asymptomatic females: detection of antibody activity to gonococcal pili antigen by radioimmunoassay. J. clin. Microbiol. 5:26
    [Google Scholar]
  11. Toshach S., Coull I., Sigurdson S., Dublenko S., Grocholski J., Linarez L. 1979; Evaluation of five serologic tests for antibody to Neisseria gonorrhoeae. Sex. trans. Dis. 6:214
    [Google Scholar]
  12. Wang S. P., Holmes K. K., Knapp J. S., Ott S., Kyzer D. D. 1977; Immunologic classification of Neisseria gonorrhoeae with micro-immunofluorescence. J. Immunol. 119:795
    [Google Scholar]
  13. Wong K. H., Arko R. J., Logan L. C., Bullard J. C. 1976; Immunological and serological diversity of Neisseria gonorrhoeae: gonococcal serotypes and their relationship with immunotypes. Infect. Immun. 14:1297
    [Google Scholar]
  14. World Health Organization 1978 Neisseria gonorrhoeae and gonococcal infections. Tech. Rep. Ser. 616 Geneva:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-15-1-1
Loading
/content/journal/jmm/10.1099/00222615-15-1-1
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error