Acquired immunity against mouse typhoid: genetic restriction and comparative efficacy of ribosomal and conventional vaccines Free

Abstract

Summary.

Strains of mice immunised with a ribosomal preparation of varied in their ability to survive an intraperitoneal challenge of virulent . Immunised nude (nu/nu), heterozygous (nu/ + ) mice, strain C57B1/6J and strain CBA/J succumbed to lethal infection whereas strains C3D2F/J, B6D2F/J and A/J, and Swiss mice were fully protected. Strains DBA/2J and C3H/HeJ were partially protected.

Enumeration of the systemic bacterial population after challenge with indicated that all immunised mouse strains were able to reduce the infectious load. was rapidly inactivated in the peritoneal cavity of immunised mice, effectively reducing the challenge and thereby limiting the number of organisms available to seed the systemic circulation. This response was also obtained in immunised athymic mice and was therefore attributed to a T-cell independent antibody response. Organisms that escaped destruction in the peritoneal cavity multiplied rapidly in the reticuloendothelial organs. Only mice from strains genetically capable of developing an effective cell-mediated immune response to the antigenic stimulus provided by the challenge organism itself survived infection.

The efficacy of ribosomal immunisation was compared with immunisation by heat-killed bacteria, viable attenuated and viable virulent organisms by enumeration of the systemic bacterial population after intravenous challenge with . Vaccination with ribosomal preparations or heat-killed organisms provided limited protection whereas immunity provided by viable organisms was far superior.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-14-4-419
1981-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/14/4/medmicro-14-4-419.html?itemId=/content/journal/jmm/10.1099/00222615-14-4-419&mimeType=html&fmt=ahah

References

  1. Ashwell G. 1957; Colorimetric analysis of sugars. In Methods in enzymology 3 Colowick S. P., Kaplan N. O. Academic Press; New York:73
    [Google Scholar]
  2. Blanden R. V., Mackaness G. B., Collins F. M. 1966; Mechanisms of acquired resistance in mouse typhoid. J. exp. Med. 124:585
    [Google Scholar]
  3. Collins F. M. 1968; Recall of immunity in mice vaccinated with Salmonella enteritidis or Salmonella typhimurium . J. Bact. 95:2014
    [Google Scholar]
  4. Collins F. M. 1969; Effect of specific immune mouse serum on the growth of Salmonella enteritidis in nonvaccinated mice challenged by various routes. J. Bact. 97:667
    [Google Scholar]
  5. Collins F. M. 1971; Mechanisms in antimicrobial immunity. J. reticuloendothel. Soc. 10:58
    [Google Scholar]
  6. Collins F. M. 1972; Effect of adjuvant on immunogenicity of a heat-killed salmonella vaccine. J. infect. Dis. 126:69
    [Google Scholar]
  7. Collins F. M. 1973; Immunogenicity of living and heat-killed Salmonella pullorum vaccines. Infect. Immun. 7:735
    [Google Scholar]
  8. Collins F. M. 1974; Vaccines and cell-mediated immunity. Bact. Rev. 38:371
    [Google Scholar]
  9. Collins F. M., Mackaness G. B., Blanden R. V. 1966; Infection-immunity in experimental salmonellosis. J. exp. Med. 124:601
    [Google Scholar]
  10. Cvjetanovic B., Uemura K. 1965; The present status of field and laboratory studies of typhoid and paratyphoid vaccines. Bull. Wld Hlth Org. 32:29
    [Google Scholar]
  11. Davies R., Kotlarski I. 1976; The role of thymus-derived cells in immunity to salmonella infection. Aust. J. exp. Biol. med. Sci. 54:221
    [Google Scholar]
  12. Dische Z. 1955; Color reactions of nucleic acid components. 285–306 In The nucleic acids 1 chemistry and biology Chargaffand E., Davidson J. N. Academic Press; New York:
    [Google Scholar]
  13. Eisenstein T. K. 1975; Evidence for O antigens as the antigenic determinants in “ribosomal” vaccines prepared from Salmonella . Infect. Immun. 12:364
    [Google Scholar]
  14. Germanier R. 1972; Immunity in experimental salmonellosis. III. Comparative immunization with viable and heat-inactivated cells of Salmonella typhimurium . Infect. Immun. 5:792
    [Google Scholar]
  15. Hobson D. 1957; Resistance to reinfection in experimental mouse typhoid. J. Hyg., Camb. 55:334
    [Google Scholar]
  16. Hoops P., Prather N. E., Berry L. J., Ravel J. M. 1976; Evidence for an extrinsic immunogen in effective ribosomal vaccines from Salmonella typhimurium . Infect. Immun. 13:1184
    [Google Scholar]
  17. Jenkin C. R., Rowley D. 1963; Basis for immunity to typhoid in mice and the question of “cellular immunity”. Bact. Rev. 27:391
    [Google Scholar]
  18. Johnson W. 1972; Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis . Infect. Immun. 5:947
    [Google Scholar]
  19. Johnson W. 1973; Ribosomal vaccines. II. Specificity of the immune response to ribosomal ribonucleic acid and protein isolated from Salmonella typhimurium . Infect. Immun. 8:395
    [Google Scholar]
  20. Lieberman M. M. 1977; Direct evidence for the presence of lipopolysaccharide components in a Pseudomonas ribosomal vaccine. Infect. Immun. 17:471
    [Google Scholar]
  21. Lin J.-H., Berry L. J. 1978; The use of strain LT2-M1 in identifying the protective antigens in a Salmonella typhimurium-derived ribosomal vaccine. J. reticuloendothel. Soc. 23:135
    [Google Scholar]
  22. Lowry O. H., Rosebrough N. J., Farr A. L., Randoll R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265
    [Google Scholar]
  23. Mackaness G. B., Blanden R. V., Collins F. M. 1966; Host-parasite relations in mouse typhoid. J. exp. Med. 124:573
    [Google Scholar]
  24. Marecki N. M., Hsu H. S., Mayo D. R. 1975; Cellular and humoral aspects of host resistance in murine salmonellosis. Br. J. exp. Path. 56:231
    [Google Scholar]
  25. Margolis J. M., Bigley N. J. 1972; Cytophilic macroglobulin reactive with bacterial protein in mice immunized with ribonucleic acid-protein fractions of virulent Salmonella typhimurium . Infect. Immun. 6:390
    [Google Scholar]
  26. Medina S., Vas S. I., Robson H. G. 1975; Effect of nonspecific stimulation on the defense mechanisms of inbred mice. J. Immun. 114:1720
    [Google Scholar]
  27. Misfeldt M. L., Johnson W. 1976; Variability of protection in inbred mice induced by a ribosomal vaccine prepared from Salmonella typhimurium . Infect. Immun. 14:652
    [Google Scholar]
  28. Misfeldt M. L., Johnson W. 1978; Identification of protective cell surface proteins in ribosomal fractions from Salmonella typhimurium . Infect. Immun. 24:808
    [Google Scholar]
  29. Mitsuhashi S., Kawakami M., Yamaguchi Y., Nagai M. 1958; Studies on the experimental typhoid. I. A comparative study of living and killed vaccines against the infection of mice with S. enteritidis . Jap. J. exp. Med. 28:249
    [Google Scholar]
  30. Nickol A. D., Bonventre P. F. 1977; Anomalous high native resistance of athymic mice to bacterial pathogens. Infect. Immun. 18:636
    [Google Scholar]
  31. Plant J., Glynn A. A. 1974; Natural resistance to Salmonella infection, delayed hypersensitivity and Ir genes in different strains of mice. Nature, Lond. 248:345
    [Google Scholar]
  32. Plant J., Glynn A. A. 1976; Genetics of resistance to infection with Salmonella typhimurium in mice. J. infect. Dis. 133:72
    [Google Scholar]
  33. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 27:493
    [Google Scholar]
  34. Robson H. G., Vas S. I. 1972; Resistance of inbred mice to Salmonella typhimurium . J. infect. Dis. 126:378
    [Google Scholar]
  35. Rowley D., Turner K. J., Jenkin C. R. 1964; The basis for immunity to mouse typhoid. 3. Cell-bound antibody. Aust. J. exp. Biol. med. Sci. 42:237
    [Google Scholar]
  36. Smith R. A., Bigley N. J. 1972; Detection of delayed hypersensitivity in mice injected with ribonucleic acid-protein fractions of Salmonella typhimurium . Infect. Immun. 6:384
    [Google Scholar]
  37. Ushiba D., Saito K., Akiyama T., Nakano M., Sugiyama T., Shirono S. 1959; Studies on experimental typhoid: bacterial multiplication and host cell response after infection with Salmonella enteritidis in mice immunized with live and killed vaccines. Jap. J. Microbiol. 3:231
    [Google Scholar]
  38. Venneman M. R., Berry L. J. 1971a; Serum-mediated resistance induced with immunogenic preparations of Salmonella typhimurium . Infect. Immun. 4:374
    [Google Scholar]
  39. Venneman M. R., Berry L. J. 1971b; Cell-mediated resistance induced with immunogenic preparations of Salmonella typhimurium . Infect. Immun. 4:381
    [Google Scholar]
  40. Venneman M. R., Bigley N. J. 1969; Isolation and partial characterization of an immunogenic moiety obtained from Salmonella typhimurium . J. Bact. 100:140
    [Google Scholar]
  41. Venneman M. R., Bigley N. J., Berry L. J. 1970; Immunogenicity of ribonucleic acid preparations obtained from Salmonella typhimurium . Infect. Immun. 1:574
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-14-4-419
Loading
/content/journal/jmm/10.1099/00222615-14-4-419
Loading

Data & Media loading...

Most cited Most Cited RSS feed