SUMMARY. The suckling-mouse assay was reliable for detecting enterotoxigenic strains of when standard conditions for growth and toxin testing were used. Enterotoxins were produced by bacteria grown in tryptone soya broth supplemented with yeast extract and aerated by shaking in an environmental incubator or water bath. When culture supernates together with dye were administered intragastrically to mice less than 6 days old, the presence of enterotoxin was assessed on the basis of a scoring system that incorporated the ratio , and production of diarrhoea. This method should facilitate the detection of enterotoxigenic strains of in epidemiological studies.


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