1887

Abstract

Summary.

The suckling-mouse assay was reliable for detecting enterotoxigenic strains of when standard conditions for growth and toxin testing were used. Enterotoxins were produced by bacteria grown in tryptone soya broth supplemented with yeast extract and aerated by shaking in an environmental incubator or water bath. When culture supernates together with dye were administered intragastrically to mice less than 6 days old, the presence of enterotoxin was assessed on the basis of a scoring system that incorporated the ratio , and production of diarrhoea. This method should facilitate the detection of enterotoxigenic strains of in epidemiological studies.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-14-4-401
1981-11-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jmm/14/4/medmicro-14-4-401.html?itemId=/content/journal/jmm/10.1099/00222615-14-4-401&mimeType=html&fmt=ahah

References

  1. Boulanger Y., Lallier R., Cousineau G. 1977; Isolation of enterotoxigenic Aeromonas from fish. Can. J. Microbiol 23:1161
    [Google Scholar]
  2. Cumberbatch N., Gurwith M. J., Langston C., Sack R. B., Brunton J. L. 1979; Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease. Infect. Immun 23:829
    [Google Scholar]
  3. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. 1972; Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J. infect. Dis 125:407
    [Google Scholar]
  4. Dobrescu L. 1978; Enterotoxigenic Aeromonas hydrophila from a case of piglet diarrhoea. Zbl. VetMed. B 25:713
    [Google Scholar]
  5. Schubert R. H. W. 1974 In Bergey’s manual of determinative bacteriology, 8th. Buchanan R. E., Gibbons N. E. Williams & Wilkins; Baltimore:345
    [Google Scholar]
  6. Stavric S., Jeffrey D. 1976; A modified bioassay for heat-stable Escherichia coli enterotoxin. Can. J. Microbiol 23:331
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-14-4-401
Loading
/content/journal/jmm/10.1099/00222615-14-4-401
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error