Phagocytosis and killing of bacteria in aerobic and anaerobic conditions Free

Abstract

Summary.

Phagocytosis and intracellular killing of several facultative anaerobes were observed in aerobic and anaerobic conditions. The presence of anaerobes affected these processes. and . were killed by phagocytes only at bacterial concentrations less than 1 × 10 cfu/ml; at higher concentrations of the anaerobes, killing of . and concomitant facultative anaerobes was inhibited. This effect appeared to be due to an interaction, in appropriate reducing conditions, between anaerobe and serum, which allowed engulfment of organisms by phagocytes but markedly impaired intracellular killing.

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1981-11-01
2024-03-28
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References

  1. Biggar W. D. 1975; Phagocytosis in patients and carriers of chronic granulomatous disease. Lancet 1:991
    [Google Scholar]
  2. Bjornson A. B., Altemeier W. A., Bjornson H. S. 1976; Comparison of the in vitro bactericidal activity of human serum and leukocytes against Bacteroides fragilis and Fusobacterium mortiferum in aerobic and anaerobic environments. Infect. Immun. 14:843
    [Google Scholar]
  3. Bjornson A. B., Bjornson H. S. 1978; Participation of immunoglobulin and the alternative complement pathway in opsonization of Bacteroides fragilis and Bacteroides thetaiotaomicron . J. infect. Dis. 138:351
    [Google Scholar]
  4. Casciato D. A., Rosenblatt J. E., Goldberg L. S., Bluestone R. 1975; In vitro interaction of Bacteroides fragilis with polymorphonuclear leukocytes and serum factors. Infect. Immun. 11:337
    [Google Scholar]
  5. Cowan S. T. 1974; Cowan and Steel’s manual for the identification of medical bacteria. , 2. Cambridge University Press; Cambridge:
    [Google Scholar]
  6. Duerden B. I., Holbrook W. P., Collee J. G., Watt B. 1976; The characterization of clinically important gram negative anaerobic bacilli by conventional bacteriological tests. J. appl. Bact. 40:163
    [Google Scholar]
  7. Gorbach S. L., Bartlett J. G. 1974; Anaerobic infections. New Engl. J. Med. 290:1177
    [Google Scholar]
  8. Hays R. C., Mandell G. L. 1974; pO2, pH, and redox potential of experimental abscesses. Proc. Soc. exp. Biol. Med. 147:29
    [Google Scholar]
  9. Ingham H. R., Dutton J., Sisson P. R., Sprott M. S., Selkon J. B. 1978; An aid to the preliminary identification of non-sporing anaerobes. J. clin. Path. 31:806
    [Google Scholar]
  10. Ingham H. R., Sisson P. R., Tharagonnet D., Selkon J. B., Codd A. A. 1977; Inhibition of phagocytosis in vitro by obligate anaerobes. Lancet 2:1252
    [Google Scholar]
  11. Light T. S. 1972; Standard solution for redox potential measurements. Analyt. Chem. 44:1038
    [Google Scholar]
  12. McRipley R. J., Sbarra A. J. 1967; Role of the phagocyte in host-parasite interactions. XI. Relationship between stimulated oxidative metabolism and hydrogen peroxide formation, and intracellular killing. J. Bact. 94:1417
    [Google Scholar]
  13. Mandell G. L. 1974; Bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils. Infect. Immun. 9:337
    [Google Scholar]
  14. Tally F. P., Goldin B. R., Sullivan N., Johnston J., Gorbach S. L. 1978; Antimicrobial activity of metronidazole in anaerobic bacteria. Antimicrob. Agents Chemother. 13:460
    [Google Scholar]
  15. Tharagonnet D., Sisson P. R., Roxby C. M., Ingham H. R., Selkon J. B. 1977; The APIZYM system in the identification of gram-negative anaerobes. J. clin. Path. 30:505
    [Google Scholar]
  16. Tofte R. W., Peterson P. K., Schmeling D., Bracke J., Kim Y., Quie P. G. 1980; Opsinization of four Bacteroides species: role of the classical complement pathway and immunoglobulin. Infect. Immun. 27:784
    [Google Scholar]
  17. Walden W. C., Hentges D. J. 1975; Differential effects of oxygen and oxidation-reduction potential on the multiplication of three species of anaerobic intestinal bacteria. Appl.Microbiol. 30:781
    [Google Scholar]
  18. Weinstein W. M., Onderdonk A. B., Bartlett J. G., Louie T. J., Gorbach S. L. 1975; Antimicrobial therapy of experimental intraabdominal sepsis. J. infect. Dis. 132:282
    [Google Scholar]
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